Animal in-vitro loading device

Abstract

The invention provides an animal in-vitro loading device which comprises a base body, a first flow guide pipe, a second flow guide pipe and a surface-enhanced Raman substrate; at least two channels penetrating through the base body are arranged in the base body; the first flow guide pipe is connected to the base body, communicates with one ends of the channels and is used for introducing fluid into the channels; the second flow guide pipe is connected to the base body, communicates with the other ends of the channels and is used for leading out fluid in the channels; the surface-enhanced Raman substrate is arranged on the inner wall of one of the passages, so that a target component in the fluid is attached to the surface of the surface-enhanced Raman substrate; and a transparent window is formed in the position, corresponding to the surface-enhanced Raman substrate, of the base body, so that Raman spectrum laser beams can penetrate through the transparent window to detect target components attached to the surface-enhanced Raman substrate. According to the invention, the bile liquid retention property is good, the influence on the liver-intestine circulation is small, the surface enhanced Raman spectrum technology is combined, a drug target component in the bile can be rapidly detected, and the efficient bile sampling and detection integration is achieved.

Description

technical field [0001] The invention belongs to the technical field of animal experiments, and in particular relates to an animal extracorporeal device capable of rapidly detecting drug target components in enterohepatic circulation bile. Background technique [0002] Bile excretion is the most important way for most drugs and their metabolites to be excreted outside the kidney. Drugs and metabolites with a molecular weight above 300 and polar groups are mainly secreted from the liver and excreted in the bile. In addition, some drugs such as diethylstilbestrol, indomethacin, chloramphenicol, erythromycin, morphine, etc. can form enterohepatic circulation. These drugs are combined with glucuronic acid in liver cells and then discharged into bile. After reaching the small intestine, it is hydrolyzed and reabsorbed, which plays an important role in the delayed excretion of drugs, which can make the pharmacokinetic curve of some drugs present the characteristics of double absorp...

AI Technical Summary

Problems solved by technology

Although this method is mature and reliable, there are problems such as long analysis time and complicated instrument operation. The target drug components in bile need to undergo a series of complicated sample preparation steps such as extraction-purification-enrichment before they can be detected by instruments.

In addition, bile is very easy to deteriorate after leaving the animal body, and the drug target components (especially unstable metabolite components) are easily degraded and lost during sample processing, which has caused many difficulties for the accurate analysis and evaluation of drug target components

[0005] In addition, studies have shown that if bile is taken out of the body for testing, the plasma half-life of the drug will be significantly shortened, thereby affecting the accuracy and effectiveness of drug efficacy evaluation

In summary, there are complex metabolic processes and absorption characteristics of drugs in enterohepatic bile, and the existing sampling and detection techniques cannot meet the research needs of real-time in situ monitoring of changes in drug components in bile

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