Growth-promoting synthetic flora for efficient colonization of tobacco root surface and screening method of growth-promoting synthetic flora
A screening method and a technology of flora, applied in the field of microbial screening, can solve the problems of little research on growth-promoting microorganisms on the root surface, difficulty in commercialization of growth-promoting bacteria, unstable biocontrol effects in the field, etc., to achieve stable growth-promoting effects, Ease of promotion and improvement of agronomic traits
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[0057] 1. Select tobacco field soil for tobacco pot experiment
[0058] Select 4 kinds of soils in the typical tobacco field in Yongzhou, Hunan, as the test soils, numbered 1, 2, 3, 4 respectively, and the pH values of the 4 kinds of test soils are 6.91, 7.49, 7.37, 7.15); the tobacco varieties are "Yunyan 87".
[0059] 2. Obtain tobacco rhizosphere soil and tobacco root surface microbiome suspension
[0060] 40 days after transplanting the tobacco seedlings, use a small steel shovel to remove the soil around the potted tobacco plants, carefully pull out the tobacco, shake off the root soil, cut off the aboveground part of the plant, and use sterile tweezers to clamp the root system in a cone filled with PBS buffer solution. Vigorously stirred in the shaped bottle, so that all the soil on the surface of the root system entered the buffer solution to obtain the tobacco rhizosphere microbiome suspension; then the root system was rinsed with PBS buffer solution, placed in a co...
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