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Growth-promoting synthetic flora for efficient colonization of tobacco root surface and screening method of growth-promoting synthetic flora

A screening method and a technology of flora, applied in the field of microbial screening, can solve the problems of little research on growth-promoting microorganisms on the root surface, difficulty in commercialization of growth-promoting bacteria, unstable biocontrol effects in the field, etc., to achieve stable growth-promoting effects, Ease of promotion and improvement of agronomic traits

Pending Publication Date: 2022-02-18
湖南省烟草公司永州市公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the large and complex microbial communities in the plant rhizosphere, a single type of functional bacteria cannot effectively colonize and grow in the rhizosphere, which limits its biocontrol function, and the field biocontrol effect is often unstable.
In addition, the functional microorganisms in the traditional research on soil microbial regulation are mainly taken from soil soil or crop rhizosphere soil (0-2mm distance from the root surface), and there are few studies on the growth-promoting microorganisms on the root surface.
Therefore, the development of a more efficient, stable and artificial synthetic flora that can colonize the root surface can provide support for the development of effective growth-promoting bacteria, so as to fundamentally break through the bottleneck of commercial promotion of growth-promoting bacteria

Method used

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  • Growth-promoting synthetic flora for efficient colonization of tobacco root surface and screening method of growth-promoting synthetic flora
  • Growth-promoting synthetic flora for efficient colonization of tobacco root surface and screening method of growth-promoting synthetic flora
  • Growth-promoting synthetic flora for efficient colonization of tobacco root surface and screening method of growth-promoting synthetic flora

Examples

Experimental program
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Effect test

Embodiment 1

[0057] 1. Select tobacco field soil for tobacco pot experiment

[0058] Select 4 kinds of soils in the typical tobacco field in Yongzhou, Hunan, as the test soils, numbered 1, 2, 3, 4 respectively, and the pH values ​​of the 4 kinds of test soils are 6.91, 7.49, 7.37, 7.15); the tobacco varieties are "Yunyan 87".

[0059] 2. Obtain tobacco rhizosphere soil and tobacco root surface microbiome suspension

[0060] 40 days after transplanting the tobacco seedlings, use a small steel shovel to remove the soil around the potted tobacco plants, carefully pull out the tobacco, shake off the root soil, cut off the aboveground part of the plant, and use sterile tweezers to clamp the root system in a cone filled with PBS buffer solution. Vigorously stirred in the shaped bottle, so that all the soil on the surface of the root system entered the buffer solution to obtain the tobacco rhizosphere microbiome suspension; then the root system was rinsed with PBS buffer solution, placed in a co...

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Abstract

The invention provides a growth-promoting synthetic flora for efficient colonization of a tobacco root surface and a screening method of the growth-promoting synthetic flora. The synthetic flora is a mixed strain of any two or two or more of ensifer, Microvirga, ineffective pseudomonas, pseudomonas plecoglossicida, paenibacillus guangzhouensis, Devosia ginsengisoli, Pseudomonas sputita, Sphingopyxis ginsengisol, sphingobacterium and Dyadobacter endophyticus. The growth-promoting synthetic flora is high in root surface colonization capacity, has the effects of promoting growth of tobacco plants and improving agronomic characters of the tobacco plants, and is easy to popularize.

Description

technical field [0001] The invention belongs to the technical field of microbial screening, and in particular relates to a growth-promoting synthetic bacterial group for efficient colonization on the root surface of tobacco and a screening method thereof. Background technique [0002] Due to the long-term and extensive use of chemical fertilizers, pesticides and frequent tillage in tobacco leaf production, a series of problems such as reduced soil productivity and frequent occurrence of diseases in tobacco fields have been caused, which have caused a series of ecological and environmental problems with regional characteristics; in addition, in the process , it is generally believed that changes in the soil microbial community structure in tobacco fields can significantly affect the agronomic traits of tobacco plants, which is an important factor restricting the sustainable development of tobacco production. In this regard, measures such as crop rotation, adding soil amendmen...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/20A01N63/27A01N63/25A01P21/00C12R1/01C12R1/38C12R1/40
CPCC12N1/20A01N63/20A01N63/27A01N63/25
Inventor 李孝刚李凤巧靳志丽刘冬晖
Owner 湖南省烟草公司永州市公司
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