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High thousand-grain-weight gene of common wheat and application of high thousand-grain-weight gene

A 1,000-grain weight, wheat technology, applied to common wheat high 1,000-grain weight gene (TaGS5-A1b-b) and its application field, can solve the problem of no polymorphism found in the promoter region, achieve early screening, save time and resources Effect

Inactive Publication Date: 2016-06-15
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, no polymorphisms were found in the coding region and promoter region of TaGW2-6D

Method used

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  • High thousand-grain-weight gene of common wheat and application of high thousand-grain-weight gene
  • High thousand-grain-weight gene of common wheat and application of high thousand-grain-weight gene
  • High thousand-grain-weight gene of common wheat and application of high thousand-grain-weight gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The investigation of the character of wheat of different varieties of embodiment 1

[0027] The inventor conducted field investigations on 166 representative local large-scale planted common wheat varieties and advanced lines from April to June every year in the Huanghuai wheat region from 2013 to 2015. The thousand-grain weight data of each variety are shown in Table 1.

[0028] Table 1 Survey table of traits of different varieties of wheat

[0029]

[0030] Table 1 Continuation 1 Survey of traits of different varieties of wheat

[0031]

[0032] Table 1 Continued 2 Survey of traits of different varieties of wheat

[0033]

[0034] Table 1 Continued 3 Survey of wheat traits of different varieties

[0035]

[0036] Table 1 Continued 4 Survey of traits of different varieties of wheat

[0037] .

Embodiment 2

[0038] Example 2 Identification of TaGS5-A1b-b Genotype Wheat Using Specific Primers

[0039] The test materials were 4 wheat varieties: Neixiang 184, Jimai 20, Gaocheng 9411, and Yumai 9. One seed was taken from each variety of wheat, and the genomic DNA of wheat grain was extracted according to the conventional method (Chen et al., 2011) as Template, PCR amplification with specific primers.

[0040] Specific primer pairs are as follows:

[0041] Upstream primer: 5'-TCATACACACATAATCCAGTCGA-3' (SEQ ID NO.3);

[0042] Downstream primer: 5'-GATCGTGGGTGTTGCATCTAT-3' (SEQ ID NO.4).

[0043] Composition of PCR reaction system: 1.5mmol / LMgCl 2 , 0.3mmol / LdNTP, 10pmol of upstream and downstream primers, 200ng template DNA, 0.5UTaq enzyme, add 1×PCR buffer (containing 10mmol / L Tris-HCl, pH9.0, 50mmol / LKCl, 1.0%TritonX-100) to constant volume to 25 μL.

[0044] PCR reaction program: first pre-denaturation at 95°C for 5 minutes; then denaturation at 94°C for 30 s, annealing at 55°C f...

Embodiment 3

[0048] Fengkang 13, Space 6, Pumai 9, and Neijiang 31 were detected using the same method as in Example 2. The results showed that the amplified products of Fengkang 13, Pumai 9, and Neijiang 31 were sequenced in the TaGS5-A1b gene. When there is no G insertion at the -1925bp position on the promoter, it is non-TaGS5-A1b-b genotype wheat; when there is a single base G insertion at the -1925bp upstream of the gene promoter after sequencing, it is TaGS5 - A1b-b genotype wheat. It can be seen from Table 1 that the thousand-grain weight of Tiankong 6 is 50.74 g, while the thousand-grain weight of Fengkang 13, Pumai 9 and Neijiang 31 are 43.88 g, 41.57 g and 32.56 g, respectively, which are the weights of TaGS5-A1b-b genotype wheat. The kernels were larger than those of non-TaGS5-A1b-b genotype wheat, therefore, the detection results were consistent with the investigation results.

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Abstract

The invention relates to a high thousand-grain-weight gene of common wheat and application of the high thousand-grain-weight gene. A specific primer pair for detecting TaGS5-A1b-b genotype of wheat is 5'-TCATACACACATAATCCAGTCGA-3' and 5'-GATCGTGGGTGTTGCATCTAT-3'; a genome DNA (Deoxyribose Nucleic Acid) of wheat to be detected is taken as a template, and the primer pair is used to perform PCR (Polymerase Chain Reaction) amplification and then sequencing; if G is inserted into -1925bp position on the upstream of a TaGS5-A1b-b gene promoter, the gene TaGS5-A1b-b exists, and otherwise, the gene TaGS5-A1b-b does not exist. The detection primers disclosed by the invention can be applied to identification of TaGS5-A1b-b genotype wheat, and assists wheat molecular breeding and the like; the detection of TaGS5-A1b-b genotype in the molecular level can be directly realized, and important effects of improving grain sizes, the yield and other agronomic characteristics of the wheat are realized.

Description

technical field [0001] The invention belongs to the field of genetic engineering and relates to a gene for controlling the grain size of wheat, in particular to a common wheat high thousand-grain weight gene (TaGS5-A1b-b) and its application. Background technique [0002] Wheat is one of the most important food crops in the world, but with the rapid growth of population, the production of food has gradually been unable to meet the growing needs of human beings. Therefore, the improvement of wheat yield has become the key to solve the food problem. So far, many yield-related QTL loci have been identified from common wheat, such as plant height, panicle length, number of grains per panicle, grain weight per panicle, grain length, grain width and thousand-grain weight. [0003] Due to the large genome (≈17.9Gb) of common wheat, it is very difficult to clone yield-related genes directly from wheat. Comparative genomics confirmed the synteny of genes between wheat and other cro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/11C12Q1/68
CPCC07K14/415C12Q1/6895C12Q2600/13
Inventor 陈锋王沙沙崔党群董中东赵磊
Owner HENAN AGRICULTURAL UNIVERSITY
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