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Method for increasing content of resveratrol and resveratrol glucoside in peanut sprouts

A technology of resveratrol and peanut sprouts, which is applied in the directions of botanical equipment and methods, fertilizers made from biological waste, and chemicals used for biological control, etc., can solve the problem of low utilization value, high extraction cost, resveratrol problems such as low alcohol content, to achieve the effect of increasing the content and promoting synthesis

Pending Publication Date: 2022-02-01
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, under normal peanut cultivation conditions, the content of resveratrol in peanuts is low, so practical problems such as high extraction costs and low utilization value have not been resolved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Extraction method of resveratrol and resveratrol glycosides:

[0017] The roots of Haihua No. 1 peanut were cut off after 48 hours of treatment. The roots are then weighed on a scale, placed in a grinding bowl, and flash-frozen in a cup of liquid nitrogen. Frozen root samples were quickly placed in a ball mill and ground at 1700 rpm for 15 s. After grinding, scrape out the root samples with an iron spoon. The ground liquid was placed in a centrifuge tube, and 10 ml of extract (80% methanol) was added. After placing the cover, gently shake the tube, put it into a centrifuge tube rack, and shake it with a constant temperature shaker at 280rpm and 40°C for 60min. The shaking solution is ultrasonicated at 50°C for 180min, and then centrifuged for 15min at a speed of 4000rpm. The centrifuged sample was slowly filtered into a 50 ml beaker, and the filtrate was further filtered into a 2 ml brown vial using an organic syringe filter (pore size: 0.22 μm). The filtrate was st...

Embodiment 2

[0019] Determination method of resveratrol and resveratrol glycosides content:

[0020] A high-performance liquid chromatograph (Agilent, 1100 series), equipped with a quaternary pump (DE11115754), a DAD (DE11113430) detector (photodiode array detection, detection wavelength 306nm) and a chromatographic column with a porosity of 5 μm (Spursil C18, 5 μm , 250×4.6mm) to determine resveratrol.

[0021] The elution method is isocratic elution, the pass rate is 100% within 15min, and the elution components are deionized water, methanol (≥99.9% Sigma-Aldrich) and acetonitrile (content [bygc]≥99.9%, ANPEL Scientific Instrument Co., Ltd., Shanghai). The volume ratio of the eluent was deionized water (99.9%), methanol (99.9%) and acetonitrile=50%, 20% and 30%. The injection volume at each time point was 20 μL, the column temperature was 30° C., and the flow rate was 0.8 ml / min.

[0022] The determination of the content of trans-resveratrol and resveratrol glycosides in the sample is...

Embodiment 3

[0024] Peanut roots were treated with ethephon for 48 hours at concentrations of 0, 2, 4, 5, 6, 7, 8 and 9 mmol / L, respectively.

[0025] The resveratrol content of each treatment was 0, 0, 1.34, 6.65, 6.51, 7.77, 6.58 and 4.7μg / g, and it can be seen that the resveratrol content of 7mmol / L treatment was the highest.

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Abstract

The invention relates to the technical field of soilless culture of sprouting vegetables, in particular to a method for increasing the content of resveratrol and resveratrol glucoside in peanut sprouts, which comprises the following steps: screening peanut seeds with full grains, soaking the peanut seeds with water at 34-36 DEG C for 8 hours, and taking out the peanut seeds; screening normal seeds, uniformly laying the seeds on a culture tray, disinfecting the seeds with 95% alcohol, covering the seeds with a disinfection towel, soaking the seeds with tap water to ensure that the seeds are moist, and continuously culturing the seeds in a dark environment at 25 DEG C for 3-4 days until the root systems of the peanut buds grow to 4-5 cm; then preparing a cultivation nutrient solution composed of ethylene and inorganic salt with a certain concentration and replacing the original tap water with the nutrient solution for cultivating the peanuts, and cultivation continues to be conducted for 2 days. The method has the advantages that the method for increasing the content of resveratrol and resveratrol glucoside in the peanut sprouts is simple in process, the culture solution is prepared from inorganic salt and ethylene together, and the content of resveratrol and resveratrol glucoside in the peanut sprouts is remarkably increased.

Description

technical field [0001] The invention relates to the technical field of soilless cultivation of sprouted vegetables, in particular to a method for increasing the content of resveratrol and resveratrol glycosides in peanut sprouts. Background technique [0002] Resveratrol and resveratrol glycosides exist in a few plants such as knotweed, grapes, and peanuts, and are secondary metabolites and derivatives synthesized by plants stimulated and induced by biological or abiotic factors. It is a stilbene stilbene polyphenol (stilbene triphenol) used to resist pathogenic infection in plants. It can remove excessive oxygen free radicals in the body, effectively delay the damage caused by peroxidation in the aging process, and effectively of anti-aging. Resveratrol is a green anti-cancer drug with anti-tumor, anti-cancer, cardiovascular protection, anti-oxidation, anti-free radical, anti-bacterial and anti-fungal, anti-inflammatory, and liver protection functions. [0003] However, u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G5/20A01N57/12A01N59/20A01N59/16A01N61/00A01P21/00A01G31/00
CPCC05D9/02C05G3/00C05G5/20A01G31/00A01N57/12A01N59/20A01N59/16A01N61/00C05F5/008C05F11/10Y02P60/21
Inventor 林国林金兰淑包学锋马鑫宇王婷婷
Owner SHENYANG AGRI UNIV
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