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A kind of Garcinia cambogia seed extract with antitumor activity and preparation method thereof

An anti-tumor activity and anti-tumor technology, which is applied in the field of medicine, can solve the problems that there is no patent disclosure of Garcinia japonica seeds, tumor cells are prone to drug resistance, and anti-tumor cells have not been reported, so as to promote protection and reproducibility. Effects of continuous use, easy purchase, and shortened extraction time

Active Publication Date: 2022-03-04
MINZU UNIVERSITY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, due to factors such as environmental pollution, population aging, and bad living habits, the incidence of cancer has been rising. Although some drugs have good therapeutic effects on tumor cells, tumor cells are prone to drug resistance, which leads to drug resistance. Therefore, it is still very urgent to find highly effective antineoplastic drugs
[0006] At present, there is no report on the biological activity research or related patents of the anti-tumor cells of the seeds of Garcinia japonica, and there is no publication of related patents on the seeds of Garcinia japonica

Method used

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  • A kind of Garcinia cambogia seed extract with antitumor activity and preparation method thereof
  • A kind of Garcinia cambogia seed extract with antitumor activity and preparation method thereof
  • A kind of Garcinia cambogia seed extract with antitumor activity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment one: the preparation embodiment of extract

[0036] A preparation method of garcinia cambogia seed extract with anti-tumor activity ( figure 1 ), including the following detailed steps:

[0037] (1) Seed peeling: Rinse the picked fresh ripe fruit with clean water, then cut it open, take out the seed, wash the surface of the seed with distilled water to remove the residual juice, then remove the seed coat to obtain the peeled seed, and break the seed into Crumble, place in a clean tray.

[0038] (2) Drying and crushing: Place the seed fragments in a vacuum freeze dryer for freeze-drying treatment. The drying conditions are as follows: temperature is -80°C, vacuum pressure is less than 0.140 mbar, and the drying time is 48 hours. The dried seed fragments are pulverized with an electric pulverizer, and the seed powder is obtained after passing through a 40-mesh sieve, and the powder is placed in a cool and dry place.

[0039](3) Extraction and drying: Weigh ...

Embodiment 2

[0041] Example 2: Example of Activity Verification of the Extract

[0042] The following is the in vitro anti-tumor cell experiment of the Garcinia macrophylla seed extract obtained according to the present invention.

[0043] The CCK8 method was used to measure the growth inhibitory activity of the Garcinia cambogia seed extract obtained in the above-mentioned embodiment 1 to 3 kinds of tumor cells, including lung cancer cell A549, cervical cancer cell Hela and gastric cancer cell HGC-27, specifically including the following steps:

[0044] (1) Cell recovery: Take out the frozen tumor cells from the -80°C refrigerator, take one tube for each type of tumor cells, and quickly transfer them to the intercellular space with an ice box, and place the cryopreserved tubes in a water bath at 37°C. Thaw the cells. In an ultra-clean workbench, transfer the thawed cells to a 50 mL centrifuge tube, add 10 mL of LDME / F-12 complete culture solution (containing 10% fetal bovine serum and 1%...

Embodiment 3

[0057] Embodiment three: the chemical composition analysis of extract

[0058] Take 200 g of the total extract prepared in Example 1, add an appropriate amount of distilled water for suspension, then add petroleum ether to remove the seed oil, then add ethyl acetate for extraction, and concentrate the extract to dryness to obtain an ethyl acetate layer extract 63.3 g. The ethyl acetate layer extract was subjected to silica gel column chromatography and gradient elution with dichloromethane / methanol (100:0~0:100, v / v) to obtain 5 main components. Purified by repeated silica gel and dextran gel (Sephadex LH-20) column chromatography, the silica gel column chromatography used dichloromethane and methanol solvent system for gradient elution, and the Sephadex LH-20 column chromatography used dichloromethane Methane / methanol (1:1, v / v) was used as the mobile phase for isocratic elution, and the obtained main components were further prepared and purified by circulating preparative l...

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Abstract

The present invention provides a pharmaceutical composition containing biflavonoids and benzophenones, which has significant inhibitory activity on tumor cells, especially Garcinia macrophyllum seed extract and its preparation method, including fresh ripe fruits, obtained from The seeds are peeled and broken into pieces; freeze-dried in a vacuum freeze dryer, and the dried seed pieces are crushed and sieved to obtain seed powder; organic solvents are added and ultrasonic-assisted extraction is carried out; the upper part after centrifugation The clear liquid is filtered with a polytetrafluoroethylene membrane, and the obtained filtrate is concentrated under reduced pressure with a rotary evaporator to obtain a dried Garcinia macrophylla seed extract. The prepared extract has extremely significant inhibitory activity on tumor cells, and is expected to be further developed as an antitumor agent. The extraction method of the present invention has low requirements on instruments and equipment, and the equipments used are common instruments, which are easy to purchase and have low configuration costs, and the operation method is also extremely simple and low in production cost, which is favorable for large-scale production and preparation.

Description

technical field [0001] The invention belongs to the field of medicine, and specifically relates to a pharmaceutical composition of biflavonoids and benzophenones, especially the extract of Garcinia macrophyllum seeds (with higher content of bisflavonoids and benzophenones) in the preparation of anti-inflammatory drugs. The application in tumor medicine, and the preparation method of the extract. Background technique [0002] Garcinia gamboge ( Garcinia xanthochymus Hook. f. ex T. Anderson) is a perennial evergreen tree plant of the genus Garcinia in the family Clusiaceae. It is naturally distributed in Yunnan and Guangxi in my country, and also in Southeast Asia, such as Myanmar and Thailand. The plant has a variety of traditional uses in our country, including edible, medicinal, ornamental and cultural values. Traditionally, the ripe fruit can be eaten directly or used in beverage making, the juice can be used as fish sauce, and the young leaves can be eaten as wild vege...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/353A61K31/352A61K31/122A61K31/085A61K31/04A61P35/00A61K36/185A61K131/00
CPCA61K31/353A61K31/352A61K31/122A61K31/085A61K31/04A61P35/00A61K36/185A61K2236/13A61K2236/15A61K2236/51A61K2236/333A61K2236/53A61K2300/00
Inventor 龙春林林锋科
Owner MINZU UNIVERSITY OF CHINA
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