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Factory phallus impudicus liquid strain and preparation method thereof

A technology for liquid strains and secondary liquid strains, applied in biochemical equipment and methods, botanical equipment and methods, microorganism-based methods, etc., can solve the problem of reducing the production efficiency of liquid strains and the growth rate of mycelium Slow, no control of bacterial contamination and other issues

Pending Publication Date: 2022-01-18
GUIZHOU INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The growth rate of mycelium of Dongsun is slow, and it is easy to form large balls during the production of liquid strains. The degree of dispersion of the balls is low, and the activity of mycelia is low; Miscellaneous bacteria will reduce the efficiency of liquid strain production, greatly reduce the quality of strains, and increase cost input

Method used

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  • Factory phallus impudicus liquid strain and preparation method thereof

Examples

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Effect test

Embodiment 1

[0022] A factory-made winter fungus liquid strain, the liquid strain includes a first-level liquid strain and a second-level liquid strain; wherein, a third-antibody drug is added to both the first-level liquid strain and the second-level liquid strain and sodium carboxymethylcellulose. Further, the three anti-drugs are penicillin, streptomycin and amphotericin B.

[0023] The preparation method of described liquid strain is specifically as follows:

[0024] 1. Preparation of the first-class liquid strain of Dongsun:

[0025] (1) Preparation of medium: according to the formula sucrose 10g / L, sodium carboxymethylcellulose 3g / L, yeast extract powder 1g / L, corn steep liquor 4g / L, filtered water 100mL; use a 300mL glass triangular flask to prepare liquid medium , sealed with a silica gel stopper and newspaper, sterilized at 121°C for 20 minutes, and added penicillin 80 U / mL, streptomycin 0.1 mg / mL, and amphotericin B 0.20 μg / mL after cooling.

[0026] (2) Inoculation and cultiv...

Embodiment 2

[0032] A factory-made winter fungus liquid strain, the liquid strain includes a first-level liquid strain and a second-level liquid strain; wherein, a third-antibody drug is added to both the first-level liquid strain and the second-level liquid strain and sodium carboxymethylcellulose. Further, the three anti-drugs are penicillin, streptomycin and amphotericin B.

[0033] The preparation method of described liquid strain is specifically as follows:

[0034] 1. Preparation of the first-class liquid strain of Dongsun:

[0035] (1) Preparation of medium: according to the formula 15g / L of sucrose, 3g / L of sodium carboxymethylcellulose, 1g / L of yeast extract powder, 4g / L of corn steep liquor, 100mL of filtered water; prepare liquid medium with a 300mL glass triangular flask , sealed with a silica gel stopper and newspaper, sterilized at 121°C for 20 minutes, and added penicillin 80 U / mL, streptomycin 0.1 mg / mL, and amphotericin B 0.20 μg / mL after cooling.

[0036] (2) Inoculati...

Embodiment 3

[0042]A factory-made winter fungus liquid strain, the liquid strain includes a first-level liquid strain and a second-level liquid strain; wherein, a third-antibody drug is added to both the first-level liquid strain and the second-level liquid strain and sodium carboxymethylcellulose. Further, the three anti-drugs are penicillin, streptomycin and amphotericin B.

[0043] The preparation method of described liquid strain is specifically as follows:

[0044] 1. Preparation of the first-class liquid strain of Dongsun:

[0045] (1) Preparation of medium: according to the formula 20g / L of sucrose, 3g / L of sodium carboxymethylcellulose, 1g / L of yeast extract powder, 4g / L of corn steep liquor, 100mL of filtered water; prepare liquid medium with a 300mL glass triangular flask , sealed with a silica gel stopper and newspaper, sterilized at 121°C for 0 min, and added penicillin 80 U / mL, streptomycin 0.1 mg / mL, and amphotericin B 0.20 μg / mL after cooling.

[0046] (2) Inoculation and...

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Abstract

The invention belongs to the technical field of edible fungus cultivation, and particularly relates to a factory phallus impudicus liquid strain and a preparation method thereof. Sodium carboxymethyl cellulose, corn steep liquor, antibiotics and the like are added into a culture medium of the phallus impudicus liquid strain, a first-stage liquid strain is subjected to shake culture in a shake flask, and a second-stage liquid strain is prepared by adopting a fermentation tank. The preparation method can prevent and control pollution of infectious microbes such as fungi and bacteria, promote rapid proliferation and uniform dispersion of the phallus impudicus liquid strain mycelium pellets, and is suitable for industrialized production of the phallus impudicus liquid strain.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, and in particular relates to a liquid fungus of industrialized winter fungus and a preparation method thereof. Background technique [0002] Dongsun, also known as bamboo fungus and sunless fungus, belongs to the fungus of the Phallus family and the genus Phallus. It is recorded in "National Chinese Herbal Medicine Compilation" and "Chinese Materia Medica" that the stipe, fungus receptacle and fruiting body of Dongsun can be used as medicine. Its medicinal properties are sweet, light, and warm in nature. . In addition, studies have shown that the extract of winter sun can reduce the death of breast cancer patients due to venous thrombosis by reducing the incidence of platelet aggregation, and has the potential to be used as an auxiliary preventive nutrition. Its fermented product can be used to treat cancer, gout and stimulate libido after treatment. It can also inhibit the g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20A01G18/40C12N1/14C12R1/645
CPCA01G18/00A01G18/20A01G18/40C12N1/14
Inventor 王芳康超杨玲
Owner GUIZHOU INST OF BIOLOGY
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