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Tumor exosome detection reagent and detection method

A technology for tumor exosomes and detection reagents, which is applied in measurement devices, immunoassays, instruments, etc., can solve the problems of long time, restricting the improvement of exosome detection efficiency, and limited yield, etc., and achieves high sensitivity and specificity. The effect of assisting early diagnosis and shortening detection time

Active Publication Date: 2022-01-04
SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
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Problems solved by technology

[0004] At present, the detection of tumor exosomes mainly relies on ultracentrifugation or kit extraction. The steps of ultracentrifugation are cumbersome and time-consuming, which restricts the detection efficiency of exosomes. The rate is limited, it is easy to cause the loss of exosomes, which affects the quality of detection

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  • Tumor exosome detection reagent and detection method
  • Tumor exosome detection reagent and detection method
  • Tumor exosome detection reagent and detection method

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Embodiment

[0042] The present invention adopts a tumor (especially pancreatic cancer) exosome detection method based on dual nano-coupling probes, and puts the prepared dual nano-coupling probes into the tumor exosome solution, that is, Glypican -1 antibody (GPC-1 antibody) and type A ephrin receptor-2 antibody (EphA2 antibody) specific antibody modified on gold nanoparticles (AuNP for short) and magnetic nanoparticles (ZnMnCo) Fe 2 o 4 @SiO 2 (abbreviated as ZCMFS) surface, both antibodies are modified by amide reaction. Get gold nanoparticles and magnetic nanoparticles with a mass ratio of 1:2, get 50uL of 2mg / mL 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and 1 mg / mL N-hydroxysuccinimide (NHS) activated the carboxyl groups on the gold nanoparticles and the EphA2 antibody respectively for about 10 minutes, then added the GPC-1 antibody and the magnetic nanoparticles ZCMFS to react at room temperature for 2 hours, so that the modified The gold nanoparticles of t...

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Abstract

The invention relates to a tumor exosome detection reagent. The tumor exosome detection reagent is composed of magnetic nanoparticles and gold nanoparticles, wherein the surfaces of the magnetic nanoparticles are modified with phosphatidylinositol proteoglycan-1 antibodies, and the surfaces of the gold nanoparticles are modified with A-type hepatic ligand protein receptor-2 antibodies. According to the tumor exosome detection method, the detection time is greatly shortened under the condition that the detection quality is guaranteed, the detection limit is low, the sensitivity and the specificity are high, the purpose of amplifying signals to detect the tumor exosome such as pancreatic cancer is achieved, and the current early diagnosis work such as pancreatic cancer can be better assisted.

Description

technical field [0001] The invention belongs to the technical field of tumor marker diagnosis in medical clinics, and in particular relates to a tumor exosome detection reagent and detection method. Background technique [0002] With the progress of modern society and the improvement of living standards, the contradiction between people's pursuit of health and the development of medicine has become increasingly prominent. Among major diseases, cancer (malignant tumor) has become the number one killer threatening human life and health. In clinical medicine, 90% of cancer cases have been diagnosed in the middle and late stages, and the best time for treatment has been missed, which has brought great difficulties to follow-up treatment. Pancreatic cancer is a classic example: early pancreatic cancer is asymptomatic and difficult to diagnose. However, the disease develops very rapidly. By the time the diagnosis is made, most of them have developed to the middle and late stage,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/553G01N33/552G01N33/551G01N21/73G01N27/626
CPCG01N33/56966G01N33/54326G01N33/54346G01N21/73G01N27/626G01N2469/10G01N2333/4722G01N2333/715
Inventor 胡萍施剑林余治国
Owner SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
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