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Compound microbial agent for preventing potato scab and preparation method thereof

A compound microbial inoculum and a technology for potato scab, applied in the field of agricultural microorganisms, can solve the problems of reducing the amount of fertilizer application, reducing the content of heavy metals, reducing and the like, and achieving the effects of inhibiting scab, increasing yield, and being convenient to use

Pending Publication Date: 2022-01-04
ZHANGYE OLYMBEL BIO TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] This application screens and obtains four strains of Bacillus subtilis, Paecilomyces lilacinus, Trichoderma harzianum and Brevibacillus spp. from nature. Bacillus subtilis can produce substances similar to cytokinins and plant growth hormones to promote the growth of potatoes , and make potatoes resistant to pathogenic bacteria; Trichoderma harzianum has strong rhizosphere ability, is easy to colonize potato roots, and produces compounds that stimulate potato growth and induce potato defense responses, improve the microbial environment of potato roots, and enhance the growth of potatoes and disease resistance, improve the yield and income of potatoes; Paecilomyces lilacinus can inhibit nematode pollution in potato roots, and the indole acetic acid products produced by metabolism can promote the growth of potato roots at low concentrations; Brevibacillus sporogenes can enhance Photosynthesis, improve the utilization rate of chemical fertilizers, reduce nitrate content, solidify some heavy metals, reduce the heavy metal content in potatoes, use Bacillus subtilis, Paecilomyces lilacinus, Trichoderma harzianum and Brevibacillus sporogenes to act together, and use glucose as Carbon source, the prepared compound microbial agent can enhance the absorption and fixation of potato roots, provide rich nutrients for potato plant growth, and can replace part of nitrogen fertilizer to improve soil, improve soil fertility, etc., and promote the growth of potato plants growth, reducing the amount of fertilizer applied, and Trichoderma harzianum makes the prepared complex microbial inoculum easy to colonize and maintain activity in the potato root system, and the complex microbial inoculum can be applied near the potato roots in the form of seed dressing and spraying, There is no need to irrigate potatoes to avoid the occurrence of a large number of pathogenic bacteria due to the high water content in the soil; in addition, the synergistic effect of Bacillus subtilis, Paecilomyces lilacinus and Trichoderma harzianum can improve the microbial environment of potato roots and increase Potato immunity can promote the enrichment of beneficial bacteria in the potato rhizosphere soil, transform the potato rhizosphere soil from "fungal type" to "bacterial type", reduce the incidence of fungal diseases, and improve the quality of potatoes

Method used

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  • Compound microbial agent for preventing potato scab and preparation method thereof
  • Compound microbial agent for preventing potato scab and preparation method thereof
  • Compound microbial agent for preventing potato scab and preparation method thereof

Examples

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Effect test

Embodiment 1

[0035] A compound microbial bacterial agent for preventing potato scab comprises the following raw materials in parts by weight: 15 g of Bacillus subtilis, 20 g of Paecilomyces lilacinus, 7 g of Trichoderma harzianum, 10 g of Brevibacillus sporogenes and 48 g of glucose.

[0036] The preparation method of above-mentioned composite microbial bacterial agent, comprises the following steps:

[0037] S1. Inoculate the strains of Bacillus subtilis, Paecilomyces lilacinus, Trichoderma harzianum and Brevibacillus rotosporus into solid medium for screening and purification respectively, and the number of viable bacteria of Bacillus subtilis is 1×10 10 CFU / g, the viable count of Paecilomyces lilacinus is 5×10 9 CFU / g, the viable count of Trichoderma harzianum is 5×10 9 CFU / g, the number of live bacteria of Brevibacillus spp. is 1×10 10 CFU / g, the purified strains were cultured and isolated to obtain single colonies of Bacillus subtilis, Paecilomyces lilacinus, Trichoderma harzianum a...

Embodiment 2

[0044] A compound microbial bacterial agent for preventing potato scab, comprising the following raw materials in parts by weight: 5 g of Bacillus subtilis, 10 g of Paecilomyces lilacinus, 15 g of Trichoderma harzianum, 20 g of Brevibacillus lateralsporosa and 50 g of glucose.

[0045] The preparation method of above-mentioned composite microbial bacterial agent, comprises the following steps:

[0046] S1. Inoculate the strains of Bacillus subtilis, Paecilomyces lilacinus, Trichoderma harzianum and Brevibacillus rotosporus into solid medium for screening and purification respectively, and the number of viable bacteria of Bacillus subtilis is 1×10 10 CFU / g, the viable count of Paecilomyces lilacinus is 5×10 9 CFU / g, the viable count of Trichoderma harzianum is 5×10 9 CFU / g, the number of live bacteria of Brevibacillus spp. is 1×10 10 CFU / g, the purified strains were cultured and isolated to obtain single colonies of Bacillus subtilis, Paecilomyces lilacinus, Trichoderma harzi...

Embodiment 3

[0053] A compound microbial bacterial agent for preventing potato scab, comprising the following raw materials in parts by weight: 10 g of Bacillus subtilis, 13 g of Paecilomyces lilacinus, 20 g of Trichoderma harzianum, 5 g of Brevibacillus lateralsporosa and 52 g of glucose.

[0054] The preparation method of above-mentioned composite microbial bacterial agent, comprises the following steps:

[0055] S1. Inoculate the strains of Bacillus subtilis, Paecilomyces lilacinus, Trichoderma harzianum and Brevibacillus rotosporus into solid medium for screening and purification respectively, and the number of viable bacteria of Bacillus subtilis is 1×10 10 CFU / g, the viable count of Paecilomyces lilacinus is 5×10 9 CFU / g, the viable count of Trichoderma harzianum is 5×10 9 CFU / g, the number of live bacteria of Brevibacillus spp. is 1×10 10 CFU / g, the purified strains were cultured and isolated to obtain single colonies of Bacillus subtilis, Paecilomyces lilacinus, Trichoderma harzi...

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Abstract

The invention relates to the technical field of agricultural microorganisms, in particular to a compound microbial agent for preventing potato scab, which is prepared from the following raw materials in parts by weight: 5-25 parts of bacillus subtilis, 5-25 parts of paecilomyces lilacinus, 5-25 parts of trichoderma harzianum, 5-25 parts of brevibacillus laterosporus and 48-52 parts of glucose. The compound microbial agent disclosed by the invention is strong in adaptability and good in stability, is beneficial to colonization and activity maintenance of microorganisms at roots of potatoes, has a relatively strong antagonistic effect on fungal diseases in rhizosphere soil of the potatoes, and promotes enrichment of beneficial bacteria at the rhizosphere of the potatoes, so that the rhizosphere soil of the potatoes is converted from a fungal type to a bacterial type, and the incidence rate of fungal diseases of potatoes is reduced; and meanwhile, the absorption effect and the fixation effect of potato root systems can be enhanced, rich nutrient substances are provided for growth of potato plants, and the yield and the quality of potatoes are improved.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a composite microbial agent for preventing potato scab and a preparation method thereof. Background technique [0002] Potato is the fourth largest food crop next to rice, wheat, and corn. Potato is planted in a wide range of areas, and its diseases are relatively complex. Potato scab is one of the important diseases affecting potato production at present, and it is common in domestic potato production bases. Potato scab mainly harms tubers. Brown spots appear on the surface of potato pieces at the initial stage, and gradually expand into brown, nearly round or irregular large spots. Most of the diseased spots are scattered on the surface of potatoes, resulting in unsightly appearance of diseased potatoes and intolerance to storage. Potato scab generally reduces potato production by 10-30%. [0003] At present, comprehensive control measures such as crop rota...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14A01N63/22A01N63/36A01N63/38C09K17/14A01P3/00A01P21/00C12R1/125C12R1/79C12R1/885C12R1/01C09K101/00
CPCC12N1/20C12N1/14A01N63/22A01N63/36A01N63/38C09K17/14C09K2101/00
Inventor 武刚刘文庆李萍薛虎平陈玉玲俞颖张丽胡海燕
Owner ZHANGYE OLYMBEL BIO TECH
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