Compound microbial agent for preventing potato scab and preparation method thereof
A compound microbial inoculum and a technology for potato scab, applied in the field of agricultural microorganisms, can solve the problems of reducing the amount of fertilizer application, reducing the content of heavy metals, reducing and the like, and achieving the effects of inhibiting scab, increasing yield, and being convenient to use
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Embodiment 1
[0035] A compound microbial bacterial agent for preventing potato scab comprises the following raw materials in parts by weight: 15 g of Bacillus subtilis, 20 g of Paecilomyces lilacinus, 7 g of Trichoderma harzianum, 10 g of Brevibacillus sporogenes and 48 g of glucose.
[0036] The preparation method of above-mentioned composite microbial bacterial agent, comprises the following steps:
[0037] S1. Inoculate the strains of Bacillus subtilis, Paecilomyces lilacinus, Trichoderma harzianum and Brevibacillus rotosporus into solid medium for screening and purification respectively, and the number of viable bacteria of Bacillus subtilis is 1×10 10 CFU / g, the viable count of Paecilomyces lilacinus is 5×10 9 CFU / g, the viable count of Trichoderma harzianum is 5×10 9 CFU / g, the number of live bacteria of Brevibacillus spp. is 1×10 10 CFU / g, the purified strains were cultured and isolated to obtain single colonies of Bacillus subtilis, Paecilomyces lilacinus, Trichoderma harzianum a...
Embodiment 2
[0044] A compound microbial bacterial agent for preventing potato scab, comprising the following raw materials in parts by weight: 5 g of Bacillus subtilis, 10 g of Paecilomyces lilacinus, 15 g of Trichoderma harzianum, 20 g of Brevibacillus lateralsporosa and 50 g of glucose.
[0045] The preparation method of above-mentioned composite microbial bacterial agent, comprises the following steps:
[0046] S1. Inoculate the strains of Bacillus subtilis, Paecilomyces lilacinus, Trichoderma harzianum and Brevibacillus rotosporus into solid medium for screening and purification respectively, and the number of viable bacteria of Bacillus subtilis is 1×10 10 CFU / g, the viable count of Paecilomyces lilacinus is 5×10 9 CFU / g, the viable count of Trichoderma harzianum is 5×10 9 CFU / g, the number of live bacteria of Brevibacillus spp. is 1×10 10 CFU / g, the purified strains were cultured and isolated to obtain single colonies of Bacillus subtilis, Paecilomyces lilacinus, Trichoderma harzi...
Embodiment 3
[0053] A compound microbial bacterial agent for preventing potato scab, comprising the following raw materials in parts by weight: 10 g of Bacillus subtilis, 13 g of Paecilomyces lilacinus, 20 g of Trichoderma harzianum, 5 g of Brevibacillus lateralsporosa and 52 g of glucose.
[0054] The preparation method of above-mentioned composite microbial bacterial agent, comprises the following steps:
[0055] S1. Inoculate the strains of Bacillus subtilis, Paecilomyces lilacinus, Trichoderma harzianum and Brevibacillus rotosporus into solid medium for screening and purification respectively, and the number of viable bacteria of Bacillus subtilis is 1×10 10 CFU / g, the viable count of Paecilomyces lilacinus is 5×10 9 CFU / g, the viable count of Trichoderma harzianum is 5×10 9 CFU / g, the number of live bacteria of Brevibacillus spp. is 1×10 10 CFU / g, the purified strains were cultured and isolated to obtain single colonies of Bacillus subtilis, Paecilomyces lilacinus, Trichoderma harzi...
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