Fermentation culture method of trichoderma and trichoderma agent
A technology of fermentation culture and Trichoderma agent, applied in the field of Trichoderma fermentation and Trichoderma agent, can solve the problem of high material cost and achieve the effects of low cost, good propagation effect and low cost
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[0060] In a preferred embodiment, a typical medium formula for cultivating biocontrol Trichoderma, the preparation method of Trichoderma agent, comprises the following steps:
[0061] 1) Seed culture of Trichoderma species: Trichoderma species is Trichoderma harzianum; Trichoderma spores are inoculated in PDA medium, the culture temperature is 25-30°C, and the light conditions are 8-16 hours of light and 8-16 hours of darkness Alternately, the culture time is 4 days;
[0062] 2) Dilute the Trichoderma spores obtained in step 1) with sterile water to be a Trichoderma spore suspension, and the dilution concentration is 1×10 3 cfu / ml-1×10 8 cfu / ml, the dilution method is hemocytometer counting, and adjust the pH to 4-7;
[0063] 3) The Trichoderma spore suspension that step 2) is obtained is carried out liquid state fermentation, and fermentation medium is potato starch processing waste nutrient solution, and inoculum size is by volume ratio Trichoderma spore suspension: potato...
Embodiment 1
[0070] A method for fermenting and cultivating Trichoderma, comprising the following steps:
[0071] 1) Seed culture of Trichoderma species: Trichoderma species is Trichoderma harzianum; Trichoderma spores are inoculated in PDA medium, the culture temperature is 25°C, the light conditions are 8 hours of light and 16 hours of darkness alternately, and the culture time is 4 sky;
[0072] 2) Dilute the Trichoderma spores obtained in step 1) with sterile water to be a Trichoderma spore suspension, and the dilution concentration is 1×10 3 cfu / ml, the dilution method is counted on a hemocytometer, and the pH is adjusted to 4;
[0073] 3) the Trichoderma spore suspension that step 2) is obtained is carried out liquid state fermentation, and liquid fermentation medium is potato starch processing waste nutrient solution, and inoculum size is by volume ratio Trichoderma spore suspension: potato starch processing waste nutrient solution is 1: 70, the culture condition is 25°C, shaker c...
Embodiment 2
[0076] A method for fermenting and cultivating Trichoderma, comprising the following steps:
[0077] 1) Seed culture of Trichoderma species: Trichoderma species is Trichoderma longibrachiae; Trichoderma spores are inoculated in PDA medium, the culture temperature is 25°C, the light conditions are 8 hours of light and 16 hours of darkness alternately, and the culture time is 4 days;
[0078] 2) Dilute the Trichoderma spores obtained in step 1) with sterile water to be a Trichoderma spore suspension, and the dilution concentration is 1×10 3 cfu / ml, the dilution method is counted on a hemocytometer, and the pH is adjusted to 4;
[0079] 3) the Trichoderma spore suspension that step 2) obtains is carried out liquid state fermentation, and the liquid fermentation culture medium is potato starch processing waste nutrient solution, and inoculum size is by volume ratio Trichoderma spore suspension: potato starch processing waste nutrient solution is 1: 70, the culture condition is 2...
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