Application of limonene in preparation of plant resistance inducer

A plant induction and limonene technology, applied in plant growth regulators, plant growth regulators, applications, etc., can solve the problems of disease resistance activity and induced plant stress resistance activity without relevant research reports

Pending Publication Date: 2022-01-04
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of agricultural use, it has been reported in the literature that limonene has a certain antibacterial effect on plant pathogenic fungi, etc., and has certain insecticidal activity on various pests, but there is no relevant research report on its ability to induce plant disease resistance and induce plant stress resistance. Its application in agriculture, horticulture and forestry needs to be further developed

Method used

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  • Application of limonene in preparation of plant resistance inducer
  • Application of limonene in preparation of plant resistance inducer
  • Application of limonene in preparation of plant resistance inducer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: Determination of limonene anti-TMV activity

[0037] (1) Protective activity

[0038] Prepare limonene solutions with a concentration of 0.02mg / mL, 0.1mg / mL and 0.5mg / mL, select healthy tobacco leaves at the 5-6 leaf stage with consistent growth, and inoculate 2000-fold diluted TMV solution 48 hours after spraying the drug, and blank control For clear water treatment, the positive control is 0.1mg / mL chitosan oligosaccharide. Each treatment was inoculated with 3 leaves and repeated 3 times. After 3 days, the number of dead spots was counted to calculate the inhibition rate.

[0039] (2) in vitro passivation activity

[0040] Prepare limonene solutions with concentrations of 0.02mg / mL, 0.1mg / mL and 0.5mg / mL, and mix them with equal volumes of 1000-fold diluted TMV solution. Place it at room temperature for 1 hour and inoculate it in the same-growing, healthy 5-6-leaf stage tobacco leaves. The blank control was water treatment, and the positive control w...

Embodiment 2

[0049] Embodiment 2: Determination of limonene-induced tobacco anti-TMV activity

[0050] The heart leaf tobacco was selected to test the induced disease resistance activity. The heart leaf tobacco can form virus scabs, and the symptom on the common tobacco is mosaic. Different symptoms use different methods to count the disease resistance. Spray 0.02 mg / mL, 0.1 mg / mL and 0.5 mg / mL limonene to the lower three leaves of tobacco at the 6-7 leaf stage with consistent growth. After 48 hours, TMV was inoculated to the unsprayed upper leaves. The blank control was water treatment, and the positive control was chitosan oligosaccharide solution. Each plant was inoculated with 2-3 leaves, each treatment included 10 tobacco plants, and the whole experiment was repeated 3 times. After 3 days, the disease index of the number of dead spots of Nicotiana cordata was counted, the formula for calculating the inhibition rate was as above, and the formula for calculating the control effect was...

Embodiment 3

[0058] Example 3: Limonene causes changes in the activity of tobacco defense enzyme POD

[0059] After spraying 0.02mg / mL, 0.1mg / mL and 0.5mg / mL LD-limonene and L-limonene to the leaves, the leaves were taken on the 1st, 3rd, 5th, 7th, 9th, and 11th days, and the defense enzymes PAL and POD were detected. Activity changes. Weigh 5 g of plant leaves, cut them into pieces and place them in a frozen mortar, add a small amount of quartz sand, and add a total of 5 mL of 0.1 mol / L, pH 5.5 acetic acid-sodium acetate buffer in 2 to 3 times. After being ground into a homogenous slurry, centrifuge at 12,000 r / min for 15 minutes at 4°C, and the supernatant is the crude enzyme extract. Take the test tube and add 3mL 25mmol / L guaiacol and 5mL enzyme extract, then add 200μL, 5mol / L H 2 o 2 The solution mixes rapidly to initiate the reaction. Using distilled water as a reference, start to record the value of the reaction system at a wavelength of 470nm at 15s of reaction, record once eve...

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Abstract

The invention discloses application of limonene in preparation of a plant resistance inducer. The application range of the plant resistance inducer comprises induction of plant disease resistance and induction of plant stress resistance. The plant disease resistance inducing activity and the plant stress resistance inducing activity of the limonene and the plant essential oil or extract containing the limonene are determined by using biological activity determination, physiological and biochemical testing and other methods, and a plant resistance inducer and a biostimulant which take the limonene as an active component are prepared and applied in fields.

Description

technical field [0001] The invention belongs to the field of plants and applications thereof, and in particular relates to the application of limonene to prepare plant inducers. Background technique [0002] Limonene is a natural monoterpene widely present in Rutaceae plants, and it is often the key substance in the action of Rutaceae volatile oils. Because limonene has a wide range of sources, is easy to extract, has a wide range of applications, and has high practical value, research on it has gradually increased and deepened in recent years, and its application scenarios have gradually expanded, covering food, medicine, agriculture, etc. [0003] Limonene is also known as limonene, dipentene, etc., and its scientific name is 1-methyl-4-isopropylcyclohexene. It is a natural functional monoterpene. It is a colorless to light yellow oily liquid at room temperature. Human lemon flavor aroma, molecular formula C 10 h 16 , with a molecular weight of 136.23, easily soluble in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N27/00A01P21/00
CPCA01N27/00
Inventor 闫合罗伟姜悦王勇贾伊娜何姗姬晓兰孔丹马志卿
Owner NORTHWEST A & F UNIV
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