Tissue culture technology for cultivating field mutant strain of Wubaiyi Qinan No.1 agilawood

Abstract

The invention discloses a tissue culture technology for cultivating a field mutant strain of Wubaiyi Qinan No.1 agilawood, and relates to the technical field of cultivation, in particular to the tissue culture technology for cultivating the field mutant strain of Wubaiyi Qinan No.1 agilawood. The technology comprises the following steps of, S1, cutting tender leaf tips, leaf stems and rootstocks of field mutant strains of Wubaiyi Qinan No.1 agilawood as explants, and disinfecting for later use; s2, performing transverse cutting and oblique cutting to form six experimental groups, and comparing and recording callus culture conditions; s3, promoting proliferation of intermediate propagule, and performing redifferentiation to promote rooting and seedling strengthening; and s4, carrying out disinfection and disease and pest resistance cultivation on the rooted seedlings till the transplanting conditions are met, and obtaining the cultivated high-quality field mutant strain of Wubaiyi Qinan No.1 agilawood. According to the technology, the explants are disinfected in advance, the influence of carried bacteria or viruses on cultivation can be effectively reduced, different cultivation groups are matched for mutual contrast, the optimal cultivation condition is determined, and the cultivation effect is improved.

Description

technical field [0001] The invention relates to the field of cultivation technology, in particular to a tissue culture technique for cultivating Wubaiyi Qinan No. 1 Agarwood mutant strain in the field. Background technique [0002] Agarwood is a kind of tree belonging to the family Daphneaceae and the genus Agarwood, with a height of 5-15 meters. The resin accumulated after the old stem is injured is commonly known as agarwood, which can be used as a raw material for spices and a special medicine for treating stomach problems; the bark fiber is flexible, white and delicate, and can be used as a raw material for high-grade paper and artificial cotton; aromatic oil can be extracted from xylem, and flowers can be made extract. [0003] Currently, there is a lack of cultivation methods for Wubai Yiqinan No. 1 wild mutant strain of Agarwood, and the cultivation method is easily affected by the external environment, which is not convenient for large-scale cultivation of Wubai Yiq...

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies of the prior art, the present invention provides a tissue culture technique for cultivating Wubai Yiqinan No. 1 Agarwood field mutant strain, which solves the existing problem of Wubai Yiqinan No. 1 Agarwood field mutant strain proposed in the above-mentioned background technology. Lack of cultivation methods, the cultivation method is easily affected by the external environment, and it is not convenient to carry out large-scale cultivation of the field mutant strain of Wubaiyi Qinan No. 1 Agarwood