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Construction method and application of flexible surface-enhanced Raman substrate

A Raman substrate, flexible surface technology, applied in Raman scattering, material excitation analysis, instruments, etc., can solve the problem of difficult to guarantee the test detection limit, test uniformity, repeatability, etc., to broaden the measurement range, repeatability Good, short test time effect

Active Publication Date: 2021-12-24
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the immature research on the preparation methods of flexible SERS substrates, many methods are difficult to guarantee the detection limit and the uniformity and repeatability of the test.

Method used

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  • Construction method and application of flexible surface-enhanced Raman substrate
  • Construction method and application of flexible surface-enhanced Raman substrate
  • Construction method and application of flexible surface-enhanced Raman substrate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] The construction of a flexible surface-enhanced Raman substrate for determining pesticides in agricultural water bodies comprises the following steps:

[0055] 1) Preparation of gold nanocolloidal particles: citrate reduction method

[0056] Prepare 25mmol / L aqueous chloroauric acid solution and 1wt% sodium citrate dihydrate solution, then heat 100mL deionized water to boiling, add 1mL chloroauric acid aqueous solution and 0.4mL sodium citrate dihydrate solution successively, and keep stirring Until the solution changes from light yellow to black, and finally to brownish red, continue to stir and cool to room temperature;

[0057] 2) Use powdered cellulose diacetate as the solute and N,N-dimethylacetamide as the solvent to prepare a casting solution with a concentration of 7wt%, stir well until the powdery sample is completely dissolved in the solvent, and cast the film The solution was allowed to stand long enough until the air bubbles were removed.

[0058] Use a sp...

Embodiment 2

[0066] The flexible surface-enhanced Raman substrates were constructed using the method described in Example 1 and stored separately.

[0067] Prepare thiram at a concentration of 10 -4 g / mL methanol solution, then diluted with water to a concentration of 10 -8 -10 -5 g / mL, drop 30 μL of the drug solution on the substrate after air-dry storage to test the Raman signal; choose to use the substrate stored in water to filter 5 mL of the drug solution after enrichment, and then air-dry to test the Raman signal.

[0068] The Raman spectrum was measured using a confocal Raman microspectrometer (SENTERRA II, Bruker, Germany). Measurement conditions: the wavelength of the laser light source was 785nm, the laser energy was 10mW, the integration time was 1s, the magnification of the objective lens was 10×, and the spectrum The test range is 50-3655cm -1 . There are 16 spectra in a single test, the average value is taken as the test result of one test, and the average spectrum is tak...

Embodiment 3

[0071] A surface-enhanced Raman substrate construction for the determination of pesticides in agricultural water, comprising the following steps:

[0072] 1) Preparation of gold nanocolloidal particles: citrate reduction method

[0073] Prepare 25mmol / L aqueous chloroauric acid solution and 1wt% sodium citrate dihydrate solution, then heat 100mL deionized water to boiling, add 1mL aqueous chloroauric acid solution and 1mL sodium citrate dihydrate solution successively, and keep stirring until The solution changed from light yellow to black, and finally to brownish red. Continue stirring to cool to room temperature.

[0074] 2) Use powdered cellulose diacetate as the solute and N,N-dimethylacetamide as the solvent to prepare a casting solution with a concentration of 7wt%, stir well until the powdery sample is completely dissolved in the solvent, and cast the film The solution was allowed to stand long enough until the air bubbles were removed.

[0075] Use a spatula to scra...

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Abstract

The invention discloses a construction method and application of a flexible surface-enhanced Raman substrate, and belongs to the technical field of surface-enhanced Raman detection. A flexible substrate takes cellulose diacetate and gold nanoparticles as raw materials, the gold nanoparticles are filtered and intercepted on a prepared cellulose diacetate film, and then p-aminothiophenol and thiram pesticides are qualitatively and quantitatively analyzed by using a dropwise adding test or filtering enrichment test method. The method for detecting pesticide residues such as thiram has the advantages of no damage, rapidness, real-time performance, high sensitivity and the like, so that an effective method is provided for rapid determination of low-concentration pesticides, and the method has practical application value.

Description

technical field [0001] The invention belongs to the technical field of surface-enhanced Raman detection, and in particular relates to a construction method and application of a flexible surface-enhanced Raman substrate. Background technique [0002] According to the "Pesticide Management Regulations of the People's Republic of China", pesticides refer to chemically synthesized or biologically derived substances used to prevent, eliminate or control diseases, insects, grasses and other harmful organisms that endanger agriculture and forestry, and to regulate the growth of plants and insects purposefully. , a substance or a mixture of several substances of other natural substances and their preparations. It is an important means of production and strategic material to ensure food supply and improve human life. According to a recent survey by Nature Geoscience, 64% of the world's land used for agriculture and food crops is at risk of pesticide contamination, with China, Japan,...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N21/01
CPCG01N21/658G01N21/01
Inventor 肖卫华于海涛吕谦韩鲁佳
Owner CHINA AGRI UNIV
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