Application of piperlongumine in preventing and treating plant pathogenic fungi
A technology of plant pathogenic fungi and piperamide, which is applied in the directions of application, plant growth regulator, botanical equipment and method, etc., can solve problems such as unreported piperamide compounds and the like
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Embodiment 1
[0028] Application of embodiment 1-piperamide in the prevention and treatment of plant diseases
[0029] 1. The source of Piperamide
[0030] Piperlongumine, Piperlongumine, CAS No. 20069-09-4, purity greater than 98%, purchased from Chengdu Effa Biotechnology Co., Ltd.
[0031] 2. Determination of inhibitory activity of Piperamide to phytopathogenic fungi
[0032] (1) The strains to be tested
[0033] Fusarium oxysporum f.sp cuben, Botrytiscirerea, Fusarium graminearum, Thanatephorus cucumeris, Alternaria alternata, R. blight of cotton ( Rhizoctomiasolani), Peronophytoora litchii, Exserohilum turcicum, Corynespora cassiicola, all of the above pathogens were tested by the Institute of Pesticide Chemical Biology, Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences Provided with applied subject groups.
[0034] (2) Test method
[0035] ①In vitro activity test
[0036] For the agricultural bactericidal activity of Piperamide, the...
Embodiment 2
[0044] The pot experiment of embodiment 2-tomato gray mold
[0045]The present invention uses Piperamide as the bacteriostatic active ingredient, carries out the living potted experiment of tomato cinerea, test method reference (Liu Xia, Li Qian, Wang Yonghong, etc. the bacteriostatic activity of metabolites of Xenobacterium nematophila YL001 bacterial strain .Plant Protection Journal), with a slight improvement, the tomato seeds were sown in flowerpots after germination, and the test began when 3 to 5 true leaves grew. Protection and therapeutic effect all establish 3 concentration treatments, namely 500, 250 and 125mg / L, every treatment repeats 3 times, every 3 pots are a treatment, meanwhile, set 50% iprodione wettable powder (200mg / L) as Drug control, clear water as blank control.
[0046] (1) The method for measuring the protective effect is as follows: spray the test liquid first, and inoculate the spore suspension of Botrytis cinerea after 24 hours.
[0047] (2) The m...
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