Application of tumor mutation load detection reagent based on circulating tumor DNA in preparation of kit for predicting T cell lymphoma prognosis
A mutation load and detection reagent technology, applied in the field of biomedicine, can solve the problems that are difficult to promote, and achieve the effect of simple and easy method, stable method and broad application prospect
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[0051] (1) Peripheral blood was collected under the premise of signing an informed consent form with the patient. Peripheral blood samples from 79 adult patients with first-onset TCL in the Lymphoma Department of Guangdong Provincial People's Hospital were collected. All samples were taken from peripheral blood heparin anticoagulation at the time of admission. This part of the research protocol has been approved by the ethics committee of the unit. At the same time, clinical data such as survival time and survival status of TCL patients were collected.
[0052] (2) Extraction of plasma circulating tumor DNA
[0053] 2.1 Add the blood sample to a 50mL centrifuge tube, centrifuge at 3000g for 5-10 minutes, absorb the upper 10mL plasma into a new 50mL centrifuge tube;
[0054] 2.2 Add 1mL QIAGEN Proteinase K to the tube;
[0055] 2.3 Add 8mL buffer ACL and mix well for 30 seconds;
[0056] 2.4 Incubate at 60°C for 30 minutes;
[0057] 2.5 Add 18mL buffer ACB to the lysate in ...
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