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Antibacterial peptide CCM7WC as well as preparation method and application thereof

An antimicrobial peptide and sequence technology, applied in the field of biomedicine, can solve the problems of low antibacterial activity, high cytotoxicity, and poor stability, and achieve the effect of small molecular weight, simple preparation method, and high stability

Pending Publication Date: 2021-12-03
苏州市第九人民医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some natural antimicrobial peptides still have problems such as low antibacterial activity, high cytotoxicity, and poor stability.

Method used

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  • Antibacterial peptide CCM7WC as well as preparation method and application thereof
  • Antibacterial peptide CCM7WC as well as preparation method and application thereof
  • Antibacterial peptide CCM7WC as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Chemical Synthesis of Antimicrobial Peptide C-CM6 from Transformed Green Sea Turtle

[0020] The green sea turtle antimicrobial peptide Cm-CATH2 is a polypeptide encoded by a gene, containing 33 amino acid residues, a molecular weight of 4089.9Da, and an isoelectric point of 12.96. The full sequence of the green sea turtle antimicrobial peptide Cm-CATH2 is: Arg 1 Arg 2 Ser 3 Arg 4 Phe 5 Gly 6 Arg 7 Phe 8 Phe 9 Lys 10 Lys 11 Val 12 Arg 13 Lys 14 Gln 15 Leu 16 Gly 17 Arg 18 Val 19 Lys 20 Arg 21 His 22 Ser 23 Arg 24 Ile 25 Thr 26 Val 27 Gly 28 Gly 29 Arg 30 met 31 Arg 32 Phe 33 (SEQ ID NO. 1). Because Cm-CATH has the characteristics of large molecular weight, high immunogenicity and toxicity, and poor stability, according to its amino acid sequence, we first shortened it by sequence cutting method to obtain the fragment Arg 1 Arg 2 Ser 3 Arg 4 Phe 5 Gly 6 Arg 7 Phe 8 Phe 9 Lys 10 Lys 11 Val 12...

Embodiment 2

[0027] CCM7WC pharmacological experiment:

[0028] 1. Determination of antibacterial activity of CCM7WC:

[0029] (1) Pick the test strains preserved on the slant and spread them evenly on the MH solid culture medium (Beijing Suo Laibao Technology Co., Ltd.) plate, place the sterilized 0.5cm diameter filter paper on the surface of the culture medium, Add dropwise 10 μl of 2 mg / ml antimicrobial peptide C-CM6 sample solution dissolved in sterilized deionized water, incubate upside down at 37°C for 18-20 hours, and observe whether the inhibition zone is formed or not. If the sample has antibacterial activity, a clear and transparent bacteriostatic zone will be formed around the filter paper, and the larger the bacteriostatic zone, the stronger the antibacterial activity of the sample.

[0030] (2) Determination of Antimicrobial Peptide CCM7WC Minimum Inhibitory Concentration (2-fold dilution method):

[0031] Select the strains with the inhibition zone in the previous experimen...

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Abstract

The invention discloses an antibacterial peptide CCM7WC as well as a preparation method and application thereof. According to the antibacterial peptide CCM7WC as well as the preparation method and application thereof, a modified body CCM7WC is designed by utilizing a molecular modification method according to an amino acid sequence of an antibacterial peptide of a green sea turtle, and the modified body has broad-spectrum efficient antibacterial activity, low cytotoxicity and high stability and furthermore has the beneficial characteristics of small molecular weight, simple structure, simple preparation method and the like.

Description

technical field [0001] The invention relates to an antibacterial peptide CCM7WC and its preparation method and application, belonging to the technical field of biomedicine. Background technique [0002] In recent years, the large-scale abuse of traditional antibiotics has led to more and more serious problems of drug resistance of pathogenic microorganisms, posing a huge threat to human health. The clinical response to drug-resistant microbial infections is to use new or alternative antibiotics that have not been used against drug-resistant microorganisms, so this requires the continuous development of new antimicrobial drugs. [0003] Antimicrobial peptides are natural small molecular polypeptides encoded by the genes of organisms, and are important molecules in the immune system of organisms. They have direct killing effects on bacteria, fungi, viruses and even protozoa. Antimicrobial peptides have the advantages of small molecular weight, simple structure, strong antibac...

Claims

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Application Information

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IPC IPC(8): C07K14/46C07K1/04C07K1/06A61K38/17A61P31/04A61P29/00A61K8/64A61Q17/00
CPCC07K14/463A23L3/3526A23K20/147A61P31/04A61P29/00A61K8/64A61Q17/005A23V2002/00A61K38/00A23V2200/10A23V2250/50
Inventor 沈根海李斌高泉根王义鹏欧阳建红
Owner 苏州市第九人民医院
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