Hydrophis cyanocinctus reforming body antibacterial peptide QHA3, preparation method and applications thereof

An antimicrobial peptide, the technology of Qinghuan sea snake, applied in the field of biomedicine, can solve the problems of antibiotic resistance of pathogenic microorganisms and threats to human health, and achieve the effect of low hemolytic activity, broad-spectrum and high-efficiency antibacterial activity, and small molecular weight

Active Publication Date: 2016-01-27
SHANDONG INT BIOTECH PARK DEV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] In recent years, the large-scale abuse of traditional antibiotics has led to more and more serious drug resistance of pathogenic microorganisms, posing a huge threat to human health.

Method used

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  • Hydrophis cyanocinctus reforming body antibacterial peptide QHA3, preparation method and applications thereof

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Experimental program
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Effect test

Embodiment 1

[0012] Chemical Synthesis of Antimicrobial Peptide QHA3 from Transformed Sea Snake

[0013] Qinghuan Sea Snake Antimicrobial Peptide QHA is a linear polypeptide encoded by a gene, containing 30 amino acid residues, with a molecular weight of 3628.5Da and an isoelectric point of 12.61. The complete sequence of Qinghuan sea snake antimicrobial peptide QHA is: Lys 1 Phe 2 Phe 3 Lys 4 Arg 5 Leu 6 Leu 7 Lys 8 Ser 9 Val 10 Arg 11 Arg 12 Ala 13 Val 14 Lys 15 Lys 16 Phe 17 Arg 18 Lys 19 Lys 20 Pro 21 Arg 22 Leu 23 Ile 24 Gly 25 Leu 26 Ser 27 Thr 28 Leu 29 Leu 30 . According to the amino acid sequence of Qinghuan sea snake antibacterial peptide QHA, the modified QHA3 was designed by molecular transformation method, and it was chemically synthesized by peptide solid-phase synthesis method. The specific preparation method is as follows:

[0014] 1. Preparation method of QHA3: According to the above amino acid sequence of QHA3, its full sequence was synthesiz...

Embodiment 2

[0020] QHA3 pharmacological experiment:

[0021] 1. Determination of antibacterial activity of QHA3:

[0022] (1) Pick the test strains stored on the slope and evenly spread them on the MH solid medium (purchased from Qingdao Haibo Biotechnology Co., Ltd.) plate, and place the sterilized 0.5cm diameter filter paper on the medium On the surface, 10 μl of 2 mg / ml antibacterial peptide QHA3 sample solution dissolved in sterilized deionized water was added dropwise, and incubated upside down at 37°C for 18-20 hours to observe whether the inhibition zone was formed or not. If the sample has antibacterial activity, a clear and transparent bacteriostatic zone will be formed around the filter paper, and the larger the bacteriostatic zone, the stronger the antibacterial activity of the sample.

[0023] (2) Determination of antibacterial peptide QHA3 minimum inhibitory concentration (Minimum Inhibitory Concentration) (2-fold dilution method):

[0024] Select the strains with the inhib...

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Abstract

The invention belongs to the technical field of biomedicine, and specifically relates to a Hydrophis cyanocinctus antibacterial peptide QHA reforming body antibacterial peptide QHA3, a preparation method and applications thereof. According to the present invention, the reforming body antibacterial peptide is the Hydrophis cyanocinctus antibacterial peptide QHA reforming body QHA3, is a straight-chain polypeptide, contains 17 amino acid residues, has an amidated C terminal, has the molecular weight of 2276.8 Da, and has the isoelectric point of 12.32; and the reforming body antibacterial peptide QHA3 has beneficial characteristics of broad spectrum and efficient antibacterial activity, small molecular weight, simple structure, low hemolytic activity, simple preparation method, and the like.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to a modified antibacterial peptide QHA3 from Hydrophiscyanocinctus (Hydrophiscyanocinctus) antibacterial peptide QHA3 and its preparation method and application. Background technique [0002] In recent years, the large-scale abuse of traditional antibiotics has led to more and more serious problems of drug resistance of pathogenic microorganisms, posing a huge threat to human health. The clinical response to drug-resistant microbial infections is to use new or alternative antibiotics that have not been used against drug-resistant microorganisms, so this requires the continuous development of new antimicrobial drugs. [0003] Antimicrobial peptides are natural small molecular polypeptides encoded by the genes of organisms, and are important molecules in the immune system of organisms. They have direct killing effects on bacteria, fungi, viruses and even protozoa. Ant...

Claims

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Application Information

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IPC IPC(8): C07K14/46A61K38/17A61K8/64A61P31/04A23K1/17
Inventor 王义鹏张淑敏曲桂武高久香谢则平
Owner SHANDONG INT BIOTECH PARK DEV
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