Fluorescent probe for rapidly detecting hydrazine compounds as well as synthesis and application of fluorescent probe

A hydrazine compound and fluorescent probe technology, applied in the field of fluorescent probes, can solve the problems of inability to realize real-time analysis and biological imaging, long detection time, expensive instruments, etc., and achieve fast and efficient detection, low synthesis cost, and low production cost Effect

Active Publication Date: 2021-12-03
LANZHOU UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The patent with publication number CN108982447B discloses a preparation method and application of a ratiometric fluorescent probe for detecting hydrazine. The molecular probe prepared by this patent can be applied to the detection of biological samples, and has low biological toxicity, good selectivity, The phenome

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  • Fluorescent probe for rapidly detecting hydrazine compounds as well as synthesis and application of fluorescent probe
  • Fluorescent probe for rapidly detecting hydrazine compounds as well as synthesis and application of fluorescent probe
  • Fluorescent probe for rapidly detecting hydrazine compounds as well as synthesis and application of fluorescent probe

Examples

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Effect test

Embodiment 1

[0039] This example provides a method for the synthesis of fluorescent probes for rapid detection of hydrazine compounds, including the preparation of A-1 and A-2, the specific steps are as follows:

[0040] The method for preparing 0.3 g of probe A-1 includes the following steps: Accurately weigh 0.60 g (1.5 mmol) of dichlorofluorescein and add it to a 100 ml round-bottomed flask, add 20 mL of dichloromethane and stir to dissolve under nitrogen protection, and then dissolve it at 0 ° C. Add 0.20mL triethylamine (1.8mmol) and 0.25mL acryloyl chloride (3mmol) successively under ice bath, mix and stir for 10min, and then react at room temperature for 12h. Remove the solvent, use petroleum ether-ethyl acetate eluent with a volume ratio of 6:1 to elute with a gradient of 25%-65% / 60min, and the flow rate during elution is 6mL / min, and the probe is obtained after column chromatography A-1, the synthetic route of probe A-1 is as follows figure 1 shown.

[0041] The method for prepa...

Embodiment 2

[0044] This example provides the application of a fluorescent probe for the rapid detection of hydrazine compounds, and illustrates that the fluorescent probe synthesized in Example 1 can detect hydrazine compounds with naked eyes.

[0045]Add 10 μL of hydrazine compound (500 μM, 50 eq) into the buffer solution containing the fluorescent probe, the buffer solution is PBS and DMSO solution with a volume ratio of 9:1, the concentration is 10 μM, and the pH is 7.4. After fully reacting for 1 h, under natural light, Compared with the blank solution, the solution system becomes obviously yellow-green after the reaction of the fluorescent probe with the analyte; under the 325nm ultraviolet lamp, it is found that the hydrazine compound shows obvious yellow-green fluorescence, which shows that the fluorescent probe synthesized in Example 1 The probe can realize naked-eye detection of hydrazine compounds.

Embodiment 3

[0047] Such as Figure 2-10 , This example provides an application of a fluorescent probe for rapid detection of hydrazine compounds, indicating that the fluorescence intensity of the fluorescent probe synthesized in Example 1 increases with the increase in the concentration of hydrazine compounds.

[0048] A-1: Add hydrazine, hydrazine sulfate, hydrazine nitrate, partial di methylhydrazine, respectively detect the change of fluorescence intensity at 523nm. The result is as Figure 3-6 As shown, the fluorescence intensity of probe A-1 increases significantly with the increase of the concentration of the analyte, and the calculated detection limits of probe A-1 for hydrazine, hydrazine sulfate, hydrazine nitrate and unsymmetrical dimethylhydrazine are respectively: 0.21 μM (6.7ppb), 0.24μM (31.2ppb), 0.21μM (19.9ppb) and 0.64μM (38.5ppb).

[0049] A-2: Add hydrazine, hydrazine sulfate, hydrazine nitrate, partial di methylhydrazine, respectively detect the change of fluoresc...

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Abstract

The invention relates to the technical field of fluorescent probes, in particular to a fluorescent probe for rapidly detecting hydrazine compounds, the fluorescent probe comprises A-1 and A-2, and the invention discloses a synthesis method of the fluorescent probe for rapidly detecting the hydrazine compounds. The invention further discloses application of the fluorescent probe for rapidly detecting the hydrazine compounds, and the application comprises a detection method. The probe can quickly respond to hydrazine, hydrazine sulfate, hydrazine nitrate and unsymmetrical dimethylhydrazine, fluorescence enhancement is remarkable, the detection limit is low, and meanwhile high anti-interference performance and selectivity are achieved.

Description

technical field [0001] The invention relates to the technical field of fluorescent probes, in particular to a fluorescent probe for rapid detection of hydrazine compounds and its synthesis and application. Background technique [0002] Hydrazine (N 2 h 4 ), also known as hydrazine, is a colorless oily liquid with a pungent odor similar to ammonia, has strong reducing properties, high alkalinity and good water solubility, and can be used in jet engines, rocket fuels and chemical production. However, hydrazine is highly toxic, and hydrazine hydrate can be mixed with any volume ratio of hydrazine and water, which is harmful to human health. Industrial production sewage usually contains hydrazine compounds such as hydrazine nitrate and hydrazine sulfate, and the random discharge will cause serious harm to the ecological environment. [0003] The patent with the publication number CN110452171A discloses a fluorescent probe for the detection of hydrazine with the parent struct...

Claims

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Application Information

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IPC IPC(8): C07D493/10C09K11/06G01N21/64
CPCC07D493/10C09K11/06G01N21/64G01N21/643C09K2211/1088G01N2021/6417
Inventor 刘相武文君于桂琴王蓓
Owner LANZHOU UNIVERSITY
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