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Application of crocin in preparation of medicine for repairing blood-brain barrier injury

A technology for blood-brain barrier damage and crocin, which can be used in drug combinations, pharmaceutical formulations, plant raw materials, etc., and can solve problems such as undiscovered

Pending Publication Date: 2021-12-03
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] As the incidence of central nervous system diseases continues to rise and the number of patients is huge, it is urgent and necessary to explore effective ways to repair the pathological damage of the blood-brain barrier. However, in addition to a few reports using bone marrow mesenchymal stem cells, glucocorticoids and other methods with great potential risks and side effects, our previous research found that the second messenger cAMP, an endogenous substance in the human body, has the ability to repair the damaged blood-brain barrier. In addition to impaired function, no natural source substance has been found that can effectively repair the blood-brain barrier

Method used

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  • Application of crocin in preparation of medicine for repairing blood-brain barrier injury
  • Application of crocin in preparation of medicine for repairing blood-brain barrier injury
  • Application of crocin in preparation of medicine for repairing blood-brain barrier injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Gastrointestinal administration of saffron significantly improves the integrity of the blood-brain barrier

[0038] The chronic social frustration model used CD-1 mice (retired mice, male, 4-6 months, purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., weighing 48±5g) and C57BL / 6J mice (male, 7 -8 weeks, purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., body weight 20±2g), in a standard feeding environment (free diet and drinking, alternating day and night, each 12 hours), adaptive feeding for one week.

[0039] Establishment of animal model: CD-1 mice were continuously screened for 3 days, and aggressive CD-1 mice were screened out for modeling. The CD-1 mice and C57 mice were separated by a transparent plastic partition with holes, and the C57 mice were placed on the same side as the CD-1 mice to be challenged every day for 10 minutes each time, and a new mouse was received every day. After the challen...

Embodiment 2

[0045] Example 2 Oral administration of crocin improves anhedonia-like depressive behavior in mice

[0046] The establishment and grouping of the animal model are the same as in Example 1. The administration method is to give crocin (grinding with 0.5% CMC-Na, 300mg / kg) by intragastric administration 2h before the modeling every day, and the control group and the model group give equal volumes of 0.5% CMC -Na.

[0047] Effects on anhedonic behavior of mice: sugar water preference experiment was used, 1% sucrose water or drinking water were given respectively, pre-adapted for 24 hours before the experiment, and the positions of the water bottles of sugar water and drinking water were changed every 12 hours, and the weight was recorded during the experiment within 12 hours at night The mice drank the weight of sugar water and drinking water respectively, and the formula for calculating the preference coefficient of sugar water was: drinking sugar water weight / (drinking sugar wat...

Embodiment 3

[0048] Example 3 Gastrointestinal administration of saffron improves the learning and memory ability of mice

[0049] C57 mice were given crocin (ground with 0.5% CMC-Na, 300 mg / kg) by intragastric administration every day for 7 consecutive days, and the control group was given an equal volume of 0.5% CMC-Na.

[0050] Effects on the learning and memory ability of mice: The water maze experiment was selected, and the mouse Morris water maze equipment included a pool with a diameter of 100 cm and a height of 38 cm, a platform with a diameter of 6 cm and a height of 14 cm, and a real-time image acquisition and online analysis system. Before the experiment, the mice were trained for 6 days, and the mice were put into the water from different quadrants every day, and the latency time of the mice climbing up the diving platform was recorded and analyzed. On the 7th day, the platform was removed, and the number of times the mice passed the platform area within 90 seconds was recorded...

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Abstract

The invention provides application of crocin in preparation of a medicine for repairing blood-brain barrier injury. By means of a recognized model that the specific blood-brain barrier of a nucleus accumbens septi brain region is damaged due to social contusion stress, it is found that crocin can obviously repair the blood-brain barrier and improve the three core indexes of blood-brain barrier damage, specifically, expression of Cldn5 of the nucleus accumbens septi brain region is increased, leakage of exogenous IL-6 to brain parenchyma of the nucleus accumbens septi brain region is reduced, and the continuity of vascular basement membranes is improved. In addition, by means of crocin administration, the pleasant sensation deficiency-like depression behavior of mice can be improved, and the learning and memory ability of the mice can be improved. The invention discloses oral crocin capable of repairing the damaged blood brain barrier, and provides a candidate medicine with a brand new mechanism for clinically repairing the blood brain barrier injury and treating related central function injury diseases.

Description

technical field [0001] The invention relates to crocin for repairing blood-brain barrier damage, and has application in treating various central function damage diseases caused by blood-brain barrier damage. Background technique [0002] The blood-brain barrier is a protective barrier between the blood circulatory system and the central nervous system, which can limit the entry of toxic substances and inflammatory factors in the blood into the central nervous system, selectively transport the nutrients needed by the brain tissue, and discharge the brain tissue The toxic substances and metabolites produced are an important barrier to maintain the homeostasis of the central nervous system. [0003] Damage to the blood-brain barrier has become a common central injury disease. After the blood-brain barrier is damaged, the toxic substances in the peripheral circulatory system can enter the central nervous system, leading to a variety of central nervous system dysfunction diseases...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/194A61P25/00A61P25/28A61K36/88A61K36/744
CPCA61K31/194A61P25/00A61P25/28A61K36/88A61K36/744
Inventor 王广基阿基业张悦
Owner CHINA PHARM UNIV
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