Probiotic microcapsule preparation based on water-in-water emulsion structure and preparation method thereof

A technology of water-in-water emulsion and probiotics, which is applied in the direction of microcapsule preparation, microsphere preparation, lactobacillus, etc., can solve the problems of preventing water transmission, not being able to dry directly, preventing the diffusion of nutrients and metabolites, etc., to increase the load Quantity, good for diffusion, wide range of emulsification temperature

Active Publication Date: 2021-12-03
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the defects of the prior art, the object of the present invention is to provide a probiotic microcapsule preparation based on a water-in-water emulsion structure and a preparation method thereof, aiming at solving the problem of preparing microcapsules prepared by embedding probiotics in a water-in-oil emulsion. Technical problems such as preventing the diffusion of nutrients and metabolites, not conducive to the survival of probiotics, preventing water transmission, and being unable to dry directly

Method used

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  • Probiotic microcapsule preparation based on water-in-water emulsion structure and preparation method thereof
  • Probiotic microcapsule preparation based on water-in-water emulsion structure and preparation method thereof
  • Probiotic microcapsule preparation based on water-in-water emulsion structure and preparation method thereof

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preparation example Construction

[0050] A kind of preparation method of the probiotic microcapsule preparation based on the water-in-water emulsion structure that the present invention proposes, such as figure 1 shown, including the following steps:

[0051] (1) After mixing the aqueous solution of the first polymer system and the aqueous solution of the second polymer system, the polymer in the first polymer system and the polymer in the second polymer system have a water holding capacity of water Different capacities lead to different distribution ratios of water in the two polymer systems, that is, the water in the polymer system with weak water holding capacity will migrate to the polymer system with strong water holding capacity; standing still to separate the phases, resulting in different volumes Two phases, wherein the bulky phase is used as the continuous phase, and the small volume phase is used as the dispersed phase; the first polymer system contains one or more water-soluble polymers, and the sec...

Embodiment 1

[0094] Take by weighing 3 grams of dextran (molecular weight is 500000Da, solubility parameter is 23.1 (Cal / cm 3 ) 0.5 ), and 5 grams of polyethylene glycol (molecular weight is 88000Da, solubility parameter is 9.38 (Cal / cm 3 ) 0.5 ), were dissolved in 100 grams of pure water to prepare respectively 3% dextran aqueous solution A (Zeta potential is -11mV) and 5% polyethylene glycol aqueous solution B (Zeta potential is -17mV) in 100 grams of pure water. According to the mass ratio of the dextran aqueous solution and the polyethylene glycol aqueous solution being 1:1, the above-mentioned solutions were mixed, and after standing, the phases were separated. The water in the polyethylene glycol system will migrate to the dextran system, and the concentration of the two will change. At this time, according to the concentration of the two phases after mixing and the phase diagram, it is determined that the dispersed phase is a polyethylene glycol-rich phase, and the continuous pha...

Embodiment 2

[0096] Take by weighing 3 grams of dextran (molecular weight is 500000Da, solubility parameter is 23.1 (Cal / cm 3 ) 0.5 ) and 10 grams of polyethylene glycol (molecular weight is 88000Da, solubility parameter is 9.38 (Cal / cm 3 ) 0.5) and, the preparation mass fraction is 3% dextran aqueous solution A (Zeta potential is-11mV) and mass fraction is 10% polyethylene glycol aqueous solution B (Zeta potential is-18mV), according to dextran aqueous solution and polyethylene glycol The mass ratio of the diol aqueous solution is 1:4, and the above solutions are mixed, and after standing, the phases are separated. The water in the polyethylene glycol system will migrate to the dextran system, and the concentration of the two will change. At this time, according to the concentration of the two phases after mixing and the phase diagram, it is determined that the dispersed phase is a polyethylene glycol-rich phase, and the continuous phase is a dextran-rich phase. The added mass fractio...

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Abstract

The invention belongs to the field of probiotic microcapsule preparations, and particularly relates to a probiotic microcapsule preparation based on a water-in-water emulsion structure and a preparation method thereof. According to the method, a water-phase continuous phase in which a particle stabilizer is dispersed and a water-phase dispersed phase containing probiotics are subjected to shear emulsification, a stable water-in-water Pickering emulsion loaded with the probiotics is prepared, and the probiotic microcapsule preparation can be obtained after the emulsion loaded with the probiotics is dried. According to the water-in-water emulsion system constructed by the method, an oil-water interface barrier existing in a traditional oil-water emulsion system is broken through, free migration of water in the drying process after embedding of the probiotics is broken through, combination of embedding and drying of the water-in-water Pickering emulsion is achieved, and particularly, the probiotic solid preparation can be directly prepared through spray drying. According to the prepared probiotic preparation, the tolerance of probiotics is improved, and the survival rate can still reach 97% or above after the probiotic preparation is stored for six months under the normal temperature condition.

Description

technical field [0001] The invention belongs to the field of probiotic microcapsule preparations, and more specifically relates to a probiotic microcapsule preparation based on a water-in-water emulsion structure and a preparation method thereof. Background technique [0002] Probiotics are a class of active microorganisms that are beneficial to the host. They are a general term for active beneficial microorganisms that colonize the human intestinal tract and reproductive system and can produce definite health effects, thereby improving the host's micro-ecological balance and exerting a beneficial effect on the intestinal tract. Beneficial bacteria or fungi in humans and animals mainly include Clostridium butyricum, lactic acid bacteria, bifidobacteria, Lactobacillus acidophilus, actinomycetes, and yeasts. At present, the most powerful products researched in the world are mainly compound active probiotics composed of the above-mentioned various microorganisms, which are wide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/135A23P10/30B01J13/02
CPCA23L33/135A23P10/30B01J13/02A23V2002/00A23V2400/121A23V2400/143A23V2400/225A23V2400/31A23V2400/125A23V2400/123A23V2400/231A23V2400/113A23V2400/513A23V2400/529A23V2400/517A23V2400/61A23V2400/533A23V2200/3204A23V2300/41A23V2300/38
Inventor 刘石林雷婵谢云霄周超一李艳梁宏闪李晶陈义杰李斌
Owner HUAZHONG AGRI UNIV
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