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A kind of antibacterial uterine horn insemination diluent suitable for donkey semen and preparation method thereof

A uterine horn and diluent technology, applied in the field of animal husbandry, can solve problems such as the inability to guarantee the conception rate, affect the health of the donkey uterus, shorten the movement distance of sperm, and achieve the effects of promoting the scale of breeding, promoting development and promotion, and promoting application and promotion

Active Publication Date: 2022-07-19
INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI +2
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AI Technical Summary

Problems solved by technology

Because the sperm's vitality will gradually decrease after leaving the male donkey's body, however, the semen diluents currently on the market can only play the role of diluting the semen, and have limited effect on maintaining the vitality of the sperm, and do not have the ability to promote sperm in vitro. Capabilization function, so the conception rate is still not guaranteed; at the same time, in order to promote the combination of sperm and egg cells, some breeders will use the method of deep horn insemination, that is, inject semen into the uterine horn of the female donkey to shorten the sperm count. However, when the vas deferens passes through the vagina, it is easy to bring bacteria in the vagina into the uterus, causing metritis in the donkey, which not only affects the health of the uterus of the donkey, but also affects the implantation of the embryo, and further affects the conception rate

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  • A kind of antibacterial uterine horn insemination diluent suitable for donkey semen and preparation method thereof

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Effect test

Embodiment 1

[0022] A bacteriostatic uterine horn insemination dilution suitable for donkey semen, comprising A solution and B solution, wherein:

[0023] Solution A consists of the following components: sodium chloride 1.52g, potassium dihydrogen phosphate 0.68g, magnesium sulfate heptahydrate 0.12g, sodium bicarbonate 0.35g, HEPES 4.76g, glucose 50g, sodium pyruvate 0.08g, sodium lactate 3.6mg, 24 g of skim milk powder, 3.75 g of taurine, 8.72 mg of hypotaurine, and 800 ml of pure water.

[0024] Solution B consists of the following components: 1 g of bovine serum albumin, 20 mg of heparin sodium, 3.14 mg of progesterone, 24 mg of cefoxitin sodium, 25 mg of amphotericin, 100 mg of polymyxin, and 100 ml of purified water.

[0025] The effect of each component in this embodiment is as follows:

[0026] Sodium chloride is mainly to control the electrolyte balance in sperm cells;

[0027] Potassium dihydrogen phosphate is mainly used to maintain osmotic pressure;

[0028] Magnesium ions i...

Embodiment 2

[0040] A preparation method of a bacteriostatic uterine horn insemination diluent suitable for donkey semen, comprising the following steps:

[0041] (1) get each component of A solution and B solution according to proportioning and mix, respectively make A solution and B solution;

[0042] (2) The A solution prepared in the step (1) is maintained for 15 to 30 minutes under the conditions of a pressure of 103.4 kPa and a temperature of 121.3 °C, and then sterilized;

[0043] (3) In a sterile environment, the B solution obtained in step (1) is filtered and sterilized with a 22 μm sterile filter and mixed with the sterilized A solution obtained in step (2), and by adding sterile Dilute the volume of pure water to 1L to obtain the antibacterial uterine horn insemination diluent.

[0044] Since bovine serum albumin, cefoxitin sodium, amphotericin and polymyxin in solution B are active substances, high temperature will destroy their functions. Therefore, in this embodiment, soluti...

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Abstract

The invention discloses a bacteriostatic uterine horn insemination diluent suitable for donkey semen, and also discloses a preparation method of the antibacterial uterine horn insemination diluent suitable for donkey semen. The advantages are: by adding an appropriate amount of taurine, hypotaurine, bovine serum albumin, heparin sodium and progesterone, it can promote sperm capacitation in vitro and maintain sperm motility. It can greatly improve the utilization rate of male donkey semen, and further increase the number of fertilized female donkeys, so that more female donkeys can be conceived and promote donkey breeding. The development and expansion of the enterprise's breeding scale will improve the economic benefits of the enterprise; by adding an appropriate amount of cefoxitin sodium, amphotericin and polymyxin, it can effectively reduce the probability of metritis in female donkeys, and ensure a healthy uterine environment for female donkeys. Promoting the application and promotion of deep angle insemination can provide a strong guarantee for improving the conception rate.

Description

Technical field: [0001] The invention relates to the field of animal husbandry, in particular to a bacteriostatic uterine horn infusion diluent suitable for donkey semen and a preparation method thereof. Background technique: [0002] As donkey meat, donkey milk, donkey glue and other products are more and more popular among consumers, the donkey breeding industry is also growing. During the estrus season of the donkey, the breeder will input the collected donkey semen to the female donkey, so that the female donkey can conceive and promote the reproduction of the donkey. In order to improve the conception rate of female donkeys, it is often necessary to inseminate female donkeys multiple times. In order to ensure the conception rate, usually the amount of sperm input per female donkey is about 200 million or more, and insemination is performed once a day, and continuous insemination is required. It takes 2 to 4 days of insemination to ensure the conception rate, that is, a...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 吴海青苏少锋李泽廷马跃军孙培陪白晋宇高俊
Owner INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI
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