Method for preparing quercitrin and kaempferol-3-O-rutinoside from Chinese redbud leaf extract

A technology of rutinoside and extract, which is applied in the field of medicine to achieve the effect of simple extraction process and large extraction amount

Active Publication Date: 2021-11-30
XUCHANG UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The contents of quercetin and kaempferol-3-O-rutinoside in Bauhinia leaves are relatively high, but there is no report of a large amount of pure quercetin or kaempferol-3-O-rutinoside extracted from Bauhinia plants

Method used

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  • Method for preparing quercitrin and kaempferol-3-O-rutinoside from Chinese redbud leaf extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for preparing quercetin and kaempferol-3-O-rutinoside from Bauhinia leaf extract, is characterized in that, comprises the steps:

[0032] (1) Take dried Bauhinia leaves as raw material, extract with 60% ethanol after crushing;

[0033] (2) concentrating the extract under reduced pressure and reclaiming the solvent to obtain the total extract of the extract;

[0034] (3) After the total extract of the extract is dissolved in water, it is sequentially extracted with petroleum ether, dichloromethane and ethyl acetate to obtain different organic layers;

[0035] (4) The ethyl acetate layer was concentrated under reduced pressure, the obtained extract was dissolved in water, applied to AB-8 macroporous adsorption resin, and gradiently eluted with different concentrations of ethanol to obtain the corresponding eluted fraction;

[0036] (5) Concentrate the eluted part with 60% ethanol under reduced pressure, dissolve in methanol, pass through a Sephadex LH-20 gel chr...

Embodiment 2

[0040] A method for preparing quercetin and kaempferol-3-O-rutinoside from Bauhinia leaf extract, is characterized in that, comprises the steps:

[0041] (1) Take 1kg dried Bauhinia leaves as raw material, extract with 75% ethanol after crushing;

[0042] (2) concentrating the extract under reduced pressure and reclaiming the solvent to obtain the total extract of the extract;

[0043] (3) After the total extract of the extract is dissolved in water, it is sequentially extracted with petroleum ether, dichloromethane and ethyl acetate to obtain different organic layers;

[0044] (4) The ethyl acetate layer was concentrated under reduced pressure, the obtained extract was dissolved in water, applied to AB-8 macroporous adsorption resin, and gradiently eluted with different concentrations of ethanol to obtain the corresponding eluted fraction;

[0045] (5) Concentrate the eluted part with 60% ethanol under reduced pressure, dissolve in methanol, pass through a Sephadex LH-20 gel...

Embodiment 3

[0054] A method for preparing quercetin and kaempferol-3-O-rutinoside from Bauhinia leaf extract, is characterized in that, comprises the steps:

[0055] (1) Take dried Bauhinia leaves as raw material, extract with 90% ethanol after crushing;

[0056] (2) concentrating the extract under reduced pressure and reclaiming the solvent to obtain the total extract of the extract;

[0057] (3) After the total extract of the extract is dissolved in water, it is sequentially extracted with petroleum ether, dichloromethane and ethyl acetate to obtain different organic layers;

[0058] (4) The ethyl acetate layer was concentrated under reduced pressure, the obtained extract was dissolved in water, applied to AB-8 macroporous adsorption resin, and gradiently eluted with different concentrations of ethanol to obtain the corresponding eluted fraction;

[0059] (5) Concentrate the eluted part with 60% ethanol under reduced pressure, dissolve in methanol, pass through a Sephadex LH-20 gel chr...

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Abstract

The invention discloses a method for preparing quercitrin and kaempferol-3-O-rutinoside from a Chinese redbud leaf extract, and belongs to the technical field of medicines. The method comprises the following steps: taking dried Chinese redbud leaves as raw materials, smashing the leaves, then extracting the leaves with 75% ethyl alcohol at the temperature of 50 DEG C, subjecting the extract to vacuum concentration, recovering the solvent, and obtaining a total extract; adding water to dissolve the total extract, and sequentially extracting with petroleum ether, dichloromethane and ethyl acetate to obtain different organic layers; carrying out vacuum concentration on the ethyl acetate layer, adding water into the obtained extract for dissolving, making the extract flow through AB-8 macroporous adsorption resin, and carrying out gradient elution by using ethanol with different concentrations to obtain corresponding elution parts; and carrying out vacuum concentration on the 60% ethanol elution part, dissolving with methanol, passing through a Sephadex LH-20 gel chromatographic column, and eluting with methanol to obtain a pure product of quercitrin and kaempferol-3-O-rutinoside. The extraction process is simple, a pure product of quercitrin and kaempferol-3-O-rutinoside is efficiently obtained, the used separation materials and solvents can be recycled, and the method is green, environmentally friendly, large in extraction amount and suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for preparing quercetin and kaempferol-3-O-rutinoside from Bauhinia leaf extract. Background technique [0002] Bauhinia leaves are the dried leaves of the plant Bauhinia genus Cercis in the family Leguminosae. Modern pharmacological studies have shown that Bauhinia leaves have significant analgesic and anti-inflammatory effects, and can enhance the ability of mice to withstand hypoxia and fatigue. Bauhinia leaf extract also has significant tyrosinase inhibitory effect, and it is expected to develop new tyrosinase inhibitors from it, which can be applied to the fields of food health care, beauty and whitening, and anti-melanoma. [0003] The natural compounds quercitrin and kaempferol-3-O-rutinoside involved in the present invention are flavonol compounds, which exist in various plants, but the content varies greatly. Modern pharmacological studies have sh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07D311/40C07H1/08C07H17/07
CPCC07D311/30C07D311/40C07H1/08C07H17/07
Inventor 舒朋华娄月月徐婷张彭湃黄继红魏夏兰臧硕张林张玉焕
Owner XUCHANG UNIV
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