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Lactobacillus paracasei for reducing cholesterol and application of lactobacillus paracasei

A technology for lowering cholesterol and by-cases, applied in the field of microbial engineering, can solve the problems of poor resistance to gastrointestinal environment, inability to exert cholesterol-lowering effect, neglecting systematic evaluation of its acid resistance, bile salt resistance, and gastrointestinal environment resistance, etc., to achieve Good cholesterol-lowering ability, lower serum cholesterol level, and improve the effect of intestinal microbial flora structure

Active Publication Date: 2021-11-26
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the research on cholesterol-lowering Lactobacillus paracasei, there is only one application document CN201510697710.9 "A Lactobacillus paracasei and Its Application" about the screening of Lactobacillus paracasei for lowering cholesterol, and these Lactobacillus paracasei for lowering cholesterol In the document, none of the screening of cholesterol-lowering Lactobacillus paracasei uses ferric ammonium sulfate method to quickly screen a large number of lactic acid bacteria, and uses the ability to tolerate the gastrointestinal environment, intestinal adhesion ability and its antibacterial activity as indicators to screen Lactobacillus paracasei with high cholesterol-lowering properties is screened. The current documents on Lactobacillus paracasei only evaluate the probiotic ability of a single strain, ignoring the systematic evaluation of its acid resistance, bile salt resistance, and gastrointestinal environment resistance. ability, which will easily lead to the obtained strains have high cholesterol-lowering ability, but have poor probiotic functions such as the ability to tolerate the gastrointestinal environment, making it impossible to exert its cholesterol-lowering effect

Method used

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  • Lactobacillus paracasei for reducing cholesterol and application of lactobacillus paracasei
  • Lactobacillus paracasei for reducing cholesterol and application of lactobacillus paracasei
  • Lactobacillus paracasei for reducing cholesterol and application of lactobacillus paracasei

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Experimental program
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Effect test

Embodiment 1

[0050] Example 1: Preliminary screening of cholesterol-lowering Lactobacillus paracasei

[0051] 1.1 Required materials

[0052] Lactic acid bacteria solid medium (MRS): peptone 10g, beef extract 5g, yeast powder 4g, glucose 20g, Tween 801mL, anhydrous sodium acetate 5g, ammonium citrate 2g, K 2 HPO 4 2g, MgSO 4 7H 2 O 0.2g, MnSO 4 h 2 O 0.05g, calcium carbonate 20g, agar 20g, distilled water 1000mL, pH 6.2, 121°C, sterilize for 20min.

[0053] Lactic acid bacteria liquid medium (MRS): peptone 10g, beef extract 5g, yeast powder 4g, glucose 20g, Tween 801mL, anhydrous sodium acetate 5g, ammonium citrate 2g, K 2 HPO 4 2g, MgSO 4 7H 2 O 0.2g, MnSO 4 h 2 O 0.05g, calcium carbonate 20g, distilled water 1000mL, pH 6.2, 121°C, sterilize for 20min.

[0054] Preparation of MRS-CHOL medium (cholesterol content: 100 μg / mL): Accurately weigh 0.1 g of cholesterol and put it into a small beaker, add 2 mL of Tween-80, stir well, add to 1 L of MRS liquid medium, and keep at ...

Embodiment 2

[0081] Embodiment 2: further screening of cholesterol-lowering Lactobacillus paracasei

[0082] 2.1 Growth curve and acid production curve

[0083] After the 5 optimal strains were activated and expanded, they were inoculated in MRS liquid medium with an inoculum size of 2% (v / v), cultured statically at 37°C, and samples were regularly taken every 2 hours to measure the concentration of the culture medium at 600nm. The absorbance value and pH value were repeated three times, and then the growth curve and acid production curve were drawn.

[0084] For the growth curve of lactic acid bacteria, the results are as follows image 3 As shown, the growth of the five strains of lactic acid bacteria conformed to the S-shaped curve, and no decay period was observed due to the determination of the OD value of the growth of the strains. Each strain began to enter the logarithmic growth phase after 3 hours, and then grew rapidly until about 12-15 hours into the stable phase. Different s...

Embodiment 3

[0152] Embodiment 3: the identification of lactic acid bacteria with the best cholesterol-lowering ability

[0153] 3.1 Colony characteristics

[0154] After Lactobacillus paracasei BY2 was cultured on MRS solid medium at 37°C for 48 hours, the colony morphology was as follows: Figure 14 Shown in A: The strain grows well, the colony is milky white and round, the diameter of the colony is 0.5-1.0 mm, the colony has protrusions, and the edges are neat.

[0155] 3.2 Morphology under microscope

[0156] The Gram stain of Lactobacillus paracasei BY2 was positive, and the cell morphology under the microscope was mostly rod-shaped, did not produce spores, and tended to form chains, without flagella, and did not move ( Figure 14 B).

[0157] 3.3 16s rDNA electrophoresis identification

[0158] Lactobacillus paracasei BY2 was subjected to 16s rDNA sequencing, and BLAST comparison was performed in NBCI. The 16s rDNA electrophoresis of Lactobacillus paracasei BY2 is as follows F...

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Abstract

The invention discloses lactobacillus paracasei for reducing cholesterol and an application of the lactobacillus paracasei. The lactobacillus paracasei is named as lactobacillus paracasei BY2, the preservation number is CGMCC NO.22571, and the lactobacillus paracasei is preserved in the China General Microbiological Culture Collection Center of Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing on May 20, 2021. The lactobacillus paracasei BY2 disclosed by the invention has relatively good endurance capacity to a gastrointestinal environment, still has relatively good cholesterol-reducing capacity after passing through the gastrointestinal tract, has relatively high intestinal adhesion and antibacterial activity, and is beneficial to maintaining normal microbial flora balance of the intestinal tract, so that the lactobacillus paracasei BY2can be used for reducing serum cholesterol, improving the intestinal microbial flora structure and inhibiting the growth and reproduction of pathogenic bacteria.

Description

technical field [0001] The invention belongs to the field of microbial engineering, in particular to a cholesterol-lowering Lactobacillus paracasei and its application. Background technique [0002] Cholesterol, also known as cholesterol, is widely present in animals and is the main component of normal tissue cells in the human body. It is within the normal range (adults: 2.9-6.0mmol / L; children: 3.1-5.2mmol / L) It has an extremely important impact on human health and physiological metabolism. It not only exists as a human nutrient, but also participates in many aspects of physiological metabolism. [0003] A large number of data show that cholesterol within the normal range is the material basis for cell membranes and nerve fibers, is a raw material for the synthesis of steroid hormones, and participates in the synthesis of 7-dehydrocholesterol. However, once the cholesterol content exceeds the normal range, it will accumulate in the vascular endothelium, and gradually accu...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A61K35/747A61P3/06A61P31/04A61P1/00C12R1/225
CPCC12N1/20A23L33/135A61K35/747A61P3/06A61P31/04A61P1/00A23V2002/00A23V2400/165A23V2200/3262A23V2200/3204Y02A50/30
Inventor 娄文勇崔华玲李梦帆徐培吴晓玲
Owner SOUTH CHINA UNIV OF TECH
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