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Method for evaluating resistance of rice variety to rice dwarf virus based on artificial inoculation

A technique of tooth leaf dwarfing and artificial inoculation, applied in rice cultivation, horticultural methods, botanical equipment and methods, etc., to achieve the effect of accelerating the process of disease-resistant breeding

Active Publication Date: 2021-11-26
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is still a lack of resistance identification methods in the field that can simulate the rice leaf dwarf virus infection in the natural environment

Method used

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  • Method for evaluating resistance of rice variety to rice dwarf virus based on artificial inoculation
  • Method for evaluating resistance of rice variety to rice dwarf virus based on artificial inoculation

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Experimental program
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Effect test

Embodiment 1

[0044] Example Anti rice ragged Dwarf rice varieties resistant to a different embodiment Identification

[0045] 1, rice varieties: Premium 63, oil accounts for 8, Nipponbare, Hirono rice, Guanghui 998

[0046] 2, rice ragged stunt virus detection method:

[0047] (1) or brown rice leaf extract tested insects tested planthopper RNA, anti-transcribed cDNA, by PCR using cDNA as a template;

[0048](2) PCR primers is: RRSV-F: 5'-GCCTTTCGTGCAATTCCC-3 ', RRSV-R: 5'-GCGTCATCA CCAAAGTTAGCC-3';

[0049] Reaction conditions (3) PCR was: 50 ℃, 30min; 94 ℃, 4min, 94 ℃, 30s; 56 ℃, 30s; 72 ℃ 45s; 35 cycles; extension 72 ℃ 10min, 12 ℃ stored;

[0050] (4) The resulting PCR product was a 1% agarose gel and detected, amplified the expected 895bp band samples characterized as positive samples, the bands were not amplified as a negative sample.

[0051] 3, Identification of resistant rice ragged Dwarf

[0052] (1) Acquisition of brown planthopper: collecting the brown planthopper nymphs or adults f...

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Abstract

The invention discloses a method for evaluating resistance of a rice variety to rice dwarf virus based on artificial inoculation. The method comprises the following steps of collecting and feeding brown planthoppers, obtaining a non-toxic brown planthopper group, preparing a poison source, preparing identified rice, feeding poison to the brown planthoppers, artificial inoculation, investigating, calculating morbidity and the like. According to the method, feeding virus transmission conditions and an evaluation system of virus transmission mediator brown planthopper are optimized, key technical indexes such as artificial inoculation virus feeding time, seedling grafting age, time and strength are determined, and technical regulations for identifying the artificial inoculation of the rice variety resistant the rice dwarf virus are formulated; reference is provided for normatively developing disease resistance identification and evaluation of the rice varieties, and a foundation is laid for further excavating disease-resistant genes, accelerating the disease-resistant breeding process and performing green comprehensive prevention and control on diseases.

Description

Technical field [0001] Technical Field The present invention relates to identification of plant resistance, particularly to a method of combating rice ragged stunt disease Evaluation of Rice Based artificial inoculation. Background technique [0002] After Rice ragged stunt disease pathogen Rice ragged stunt virus (Riceragged stunt virus, RRSV), mainly for mass poisoning in a lasting way by the proliferation of migratory pests brown planthopper, rice growth period to infect damage, susceptible small plant, increase high tillering, tillering, tip twist, dark green, serrated leaf margin notch, leaf sheath and leaf veins appear swollen, severe can not be normal heading strong. In 1978, my country's Fujian Province, found and reported the disease, followed by Guangdong, Jiangxi and other places are starting to have been reported. The outbreak of the disease was the last century disaster, after tends to occur sporadically. Until after 2006, the outbreak of the disease disaster in nort...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033A01G7/06A01G22/22
CPCA01K67/033A01G7/06A01G22/22
Inventor 李战彪秦碧霞谢慧婷崔丽贤蔡健和陈锦清
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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