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Hepatobilic acid detection kit and preparation method thereof

A detection kit, hepatobiliary technology, applied in the measurement of color/spectral characteristics, etc., can solve the problems of unstable absorbance change, protein agglomeration and low sensitivity, etc., and achieve the effect of simple operation, low sensitivity and high precision.

Pending Publication Date: 2021-11-23
WHITMAN BIOTECH NANJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, in clinical application, the detection method of glycocholic acid using a biochemical analyzer is mainly the latex immunoturbidimetric method. When this method is used to detect samples, due to the reaction between hepatic acid CG and the latex particle reagent coated with anti-hepatic acid CG antibody, the Aggregation phenomenon, such that protein group aggregation will occur, which will affect the unstable absorbance change and low sensitivity in the transmission turbidimetric detection

Method used

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  • Hepatobilic acid detection kit and preparation method thereof
  • Hepatobilic acid detection kit and preparation method thereof
  • Hepatobilic acid detection kit and preparation method thereof

Examples

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Embodiment 1

[0034] The serum amyloid A detection kit provided in this embodiment includes reagent R1 and reagent R2, wherein the R1 reagent includes:

[0035]

[0036] Described R2 reagent comprises:

[0037]

Embodiment 2

[0039] The serum amyloid A detection kit provided in this embodiment includes reagent R1 and reagent R2, wherein the R1 reagent includes:

[0040]

[0041] Described R2 reagent comprises:

[0042]

Embodiment 3

[0044] The serum amyloid A detection kit provided in this embodiment includes reagent R1 and reagent R2, wherein the R1 reagent includes:

[0045]

[0046]

[0047] Described R2 reagent comprises:

[0048]

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Abstract

The invention relates to the technical field of in-vitro diagnostic reagents, in particular to a hepatobilic acid detection kit. The detection kit comprises a reagent R1 and a reagent R2, the reagent R1 comprises a buffer solution, an ionic solution, a surfactant, brominated ethylene dimethylamine, polyethylene glycol and a preservative; and the R2 reagent comprises latex particles coated with a hypersensitized anti-hepatocholic acid antibody, a buffer solution, a preservative and a suspending aid. The hepatobilic acid detection kit can effectively avoid huddling type coagulation of protein so as to improve the detection sensitivity and accuracy.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents, in particular to a detection kit for hepatocholic acid and a preparation method thereof. Background technique [0002] Cholyglycine (CG) is one of the conjugated cholic acids formed by the combination of bile acid and glycine. The normal metabolic pathway of CG is the entero-hepatic circulation, which is synthesized by liver cells, discharged into the gallbladder through the capillary and bile ducts, and enters the duodenum with bile to help digest food. 95% of bile acid is reabsorbed in the terminal ileum, and then returned to the liver through the portal vein, where it is taken up and reused by liver cells. In the serum, it mainly exists in the form of protein binding, and the total amount spilled into the systemic circulation is less than 1%. Under normal circumstances, the bile acid content in peripheral blood is very small, and the serum CG concentration is stable at a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 常俊骏董文勇
Owner WHITMAN BIOTECH NANJING
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