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Color developing solution for ultraviolet visual detection and application thereof

A technology for visual detection and color developing solution, which is applied in the visual detection of color developing solution and application fields, can solve the problems of unfavorable ultraviolet protection analysis, high cost of color developing molecules, complex color developing system, etc., and achieves low price and high sensitivity. , the effect of simple reaction system

Active Publication Date: 2021-11-23
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the chromogenic system is still relatively complicated, the cost of chromogenic molecules is relatively high, and some substances have certain toxicity, which is not conducive to the promotion of personal UV protection analysis.

Method used

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  • Color developing solution for ultraviolet visual detection and application thereof
  • Color developing solution for ultraviolet visual detection and application thereof
  • Color developing solution for ultraviolet visual detection and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Dissolve 3,3',5,5'-tetramethylbenzidine (TMB) in dimethyl sulfoxide, and prepare 40mM TMB mother solution in the dark for future use, and prepare pH 3.5, 4.0, 4.5 at the same time , 5.0, 5.5 citric acid-sodium citrate buffer solution, set aside;

[0030] (2) diluting the TMB mother liquor with citric acid-sodium citrate buffer solution to a chromogenic solution whose concentration of 3,3',5,5'-tetramethylbenzidine is 0.4mM;

[0031] (3) The chromogenic solutions with different pH were exposed to light for 5 minutes under a xenon lamp (302nm), and then the absorbance at 652nm was measured by a spectrophotometer. The results are shown in Table 1.

[0032] Table 1

[0033] pH value TMB concentration (mM) Chromogenic absorbance Example 1 3.5 0.4 0.898 Example 2 4.0 0.4 1.024 Example 3 4.5 0.4 1.004 Example 4 5.0 0.4 0.872 Example 5 5.5 0.4 0.593

Embodiment 6

[0034] Examples 6–12

[0035] (1) Dissolve 3,3',5,5'-tetramethylbenzidine (TMB) in dimethyl sulfoxide, and prepare 40mM TMB mother solution in the dark for future use, and prepare citric acid with a pH of 4.0- Sodium citrate buffer solution, for subsequent use;

[0036] (2) Dilute TMB mother liquor with citric acid-sodium citrate buffer solution to 3,3',5,5'-Tetramethylbenzidine concentration is 0.02,0.05,0.1,0.2,0.4,0.6,0.8mM color liquid;

[0037] (3) Color developing solutions with different concentrations were developed under xenon lamp (302 nm) light for 2 minutes, and then the absorbance at 652 nm was measured by a spectrophotometer, and the results are shown in Table 2.

[0038] Table 2

[0039] TMB concentration (mM) pH value Chromogenic absorbance Example 6 0.02 4.5 0.194 Example 7 0.05 4.5 0.247 Example 8 0.1 4.5 0.381 Example 9 0.2 4.5 0.439 Example 10 0.4 4.5 0.460 Example 11 0.6 4.5 0.467 Exampl...

Embodiment 13

[0045] (1) Dissolving TMB in dimethyl sulfoxide, preparing a 40mM mother solution protected from light for subsequent use, and preparing a citric acid-sodium citrate buffer solution with a pH of 4.5;

[0046] (2) Add the TMB mother solution to the above-mentioned buffer solution, and dilute the chromogenic solution whose TMB concentration is 0.4mM;

[0047] (3) The chromogenic solution was exposed to four different types of ultraviolet light sources, namely xenon lamp, LED lamp, halogen lamp, and sunlight, for 5 minutes to develop color, and then the absorbance at 652 nm was measured by a spectrophotometer. The results are shown in Table 3.

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Abstract

The invention discloses a color developing solution for ultraviolet visual detection and application thereof, and the color developing solution is prepared by dissolving 3, 3 ', 5, 5'-tetramethyl benzidine in dimethyl sulfoxide to prepare a mother solution, and diluting the mother solution with a citric acid-sodium citrate buffer solution with the pH value of 3.5-5.5 until the concentration is 0.02-0.8 mM. The substrate of the color developing solution has specific response to ultraviolet light, is high in sensitivity, safe and non-toxic, can directly generate color change and is hardly influenced by visible light and infrared light, and the color development is gradually enhanced along with the accumulation of ultraviolet radiation time, so that not only can the visual detection of the ultraviolet light be accurately realized, and the color developing solution is also particularly suitable for visual analysis and detection of bracelet type portable ultraviolet detection sensors, and harm of ultraviolet light to human skin is reduced.

Description

technical field [0001] The invention belongs to the technical field of visual detection chromogenic liquid and its application, and in particular relates to a chromogenic liquid for ultraviolet visual detection and its application. Background technique [0002] Ultraviolet (UV) refers to radiated light with a wavelength ranging from 10-400nm, and ultraviolet rays of different wavelengths have different degrees of damage to the human body. Ultraviolet rays (UVA) with a wavelength of 320-400nm will accelerate the skin aging process; long-term exposure of the human body to ultraviolet rays (UVB) with a wavelength of 280-320nm will cause melanin deposition and increase the risk of skin cancer; 200-280nm ultraviolet rays (UVC) It has the highest energy, and once it irradiates a living body, it will cause fatal damage. [0003] Sunlight contains a large amount of visible light, infrared light and a small amount of ultraviolet rays. The ultraviolet rays that people are exposed to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01J1/38
CPCG01J1/38
Inventor 吴鹏张晓李显明郎云贺
Owner SICHUAN UNIV
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