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Microbial fertilizer suitable for saline alkali soil and preparation method thereof

A technology for microbial fertilizer and saline-alkali soil, which is applied to the field of microbial fertilizer for saline-alkali soil and its preparation, can solve the problems of high treatment cost, unsuitable microbial fertilizer, and poor effective microbial colonization ability.

Pending Publication Date: 2021-10-08
东营市垦利区万隆农林经贸有限公司 +1
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0008] (1) Most of the methods of controlling saline-alkali land by physical and chemical methods are relatively expensive, and it is difficult to solve the problem of saline-alkali land effectively and permanently
[0009] (2) The effective microorganisms in ordinary microbial fertilizers have poor colonization ability in saline-alkali soil, and the saline-alkali environment is not conducive to their growth and reproduction, thereby reducing the application effect of microbial fertilizers. When applied in saline-alkali soil, the promotion of crops The effect of growth and disease resistance is not ideal
[0010] (3) There are few saline-alkali-resistant strains of Bacillus velei isolated from saline-alkali soil, which are not suitable for the production of microbial fertilizers for saline-alkali soil

Method used

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  • Microbial fertilizer suitable for saline alkali soil and preparation method thereof
  • Microbial fertilizer suitable for saline alkali soil and preparation method thereof
  • Microbial fertilizer suitable for saline alkali soil and preparation method thereof

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preparation example Construction

[0047] Such as figure 1 Shown, the preparation method that is applicable to the microbial fertilizer of saline-alkali soil that the embodiment of the present invention provides comprises the following steps:

[0048] S101, activation of Bacillus veleisi;

[0049] S102, the preparation of the seed solution of the triangular flask of Bacillus Veles;

[0050] S103, preparation of Bacillus Veles liquid fermenter seeds;

[0051] S104, solid-state fermentation culture of Bacillus Velez;

[0052] S105, preparation of Bacillus megaterium bacteria powder;

[0053] S106, preparation of microbial fertilizer.

Embodiment 1

[0056] The invention discloses a microbial fertilizer suitable for saline-alkali soil and a preparation method thereof. The microbial fertilizer suitable for saline-alkali soil is prepared by using a salt-alkali-resistant and disease-resistant growth-promoting bacterium Bacillus velezensis BOB-2, a strain of Bacillus megaterium ACCC04314, and mineral humic acid. made. The Bacillus Velez BOB-2 strain has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC No.22606. Excellent characteristics or beneficial effects such as crop pathogenic bacteria can be used to produce microbial fertilizers, especially microbial fertilizers suitable for saline-alkali soils. The original species of Bacillus megaterium ACCC04314 was purchased from the China Agricultural Microorganism Culture Collection and Management Center, and its preservation number is ACCC04314.

[0057] The microbial fertili...

Embodiment 2

[0063] Example 2: Screening of Bacillus Velez BOB-2

[0064] (1) Isolation of saline-alkali-tolerant strains

[0065] Collect saline-alkali soil, weigh 10g of soil sample, place it in a sterilized 250mL Erlenmeyer flask filled with 90mL deionized water and 10-20 glass beads, and shake it on a shaker at 180r / min for 30min. Dilute the soil suspension to 10 by serial dilution -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 , 10 -7 . Use a pipette gun to draw 0.1 mL of soil suspension with different dilutions on the LB medium plate, and spread evenly. Put it in a 37°C incubator and cultivate for 48-72h. Strains with differences in colony morphological characteristics were picked and streaked on the separation plate, and separated and purified by repeated streaking until a single colony was obtained. The formula of the LB medium is: tryptone 10g / L; yeast extract 5g / L; sodium chloride (NaCl) 10g / L; agar 20g / L, pH 7.5.

[0066] The isolated strains were inoculated in LB saline-alkali me...

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Abstract

The invention belongs to the technical field of agricultural biology, and discloses a microbial fertilizer suitable for saline alkali soil and a preparation method thereof.The microbial fertilizer suitable for the saline alkali soil is prepared from, by mass, 100 parts of bacillus velezensis BOB-2 bacterial powder, 20 parts of bacillus megaterium bacterial powder, 50 parts of mineral source humic acid powder, 10 parts of zinc sulfate heptahydrate, 10 parts of magnesium sulfate heptahydrate, and 10 parts of ferrous sulfate heptahydrate. The bacillus velezensis BOB-2 strain and the bacillus megaterium ACCC04314 are taken as effective bacteria, and the bacillus velezensis BOB-2 strain is separated from saline-alkali soil, has excellent characteristics or beneficial effects of resisting saline and alkaline, promoting crop growth, improving soil enzyme activity, antagonizing crop pathogenic bacteria and the like, and can be used for producing a microbial fertilizer to obtain a better application effect.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a microbial fertilizer suitable for saline-alkali soil and a preparation method thereof. Background technique [0002] At present, my country has a large amount of saline-alkali land, mainly distributed in the northwest, north, northeast and coastal areas, with a total area of ​​about 400-500 million mu. When the soil salt content is higher than 0.2%-0.5%, it will have obvious adverse effects on plant growth. Excessively high salinity will cause the imbalance of soil water and salt movement and change of soil structure, resulting in increased soil bulk density, decreased soil particle gap, weakened soil permeability, and increased soil permeability, resulting in soil compaction, decreased fertility, and plant growth. The root system has difficulty absorbing water, and even cannot grow, which eventually leads to crop yield reduction or even abandonment. Ther...

Claims

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Application Information

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IPC IPC(8): C05G3/80
CPCC05G3/80C05D5/00C05D9/02C05F11/02C05F11/08
Inventor 刘丽英邵光杰古今朝孙中涛侯汉学崔树君徐燕徐风英
Owner 东营市垦利区万隆农林经贸有限公司
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