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Orchid seed-fungus symbiotic germination complex as well as preparation method and application thereof

A technology for plant seeds and symbiotic germination, applied in the biological field, can solve problems such as easy pollution and death of protocorm, achieve the effects of easy operation, improve germination rate and seedling rate, and shorten production time

Inactive Publication Date: 2021-10-08
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such artificial seeds are equivalent to a miniature sterile in vitro culture system, and the protocorms continue to differentiate into seedlings in the medium, but this kind of production process is easy to pollute, resulting in the death of the protocorms

Method used

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  • Orchid seed-fungus symbiotic germination complex as well as preparation method and application thereof
  • Orchid seed-fungus symbiotic germination complex as well as preparation method and application thereof
  • Orchid seed-fungus symbiotic germination complex as well as preparation method and application thereof

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preparation example Construction

[0044] The present invention provides a kind of preparation method of orchidaceae seed-fungus symbiotic germination complex, is characterized in that, comprises the following steps:

[0045] 1) preparing the mycelium of the symbiotic fungus into a suspension of fungal mycelium;

[0046] 2) mixing the fungal mycelium suspension in step 1) with the orchid seeds to obtain the orchid seed-fungus mixed suspension;

[0047] 3) Mixing the orchidaceae seed-fungus mixed suspension described in step 2) with the sodium alginate aqueous solution to obtain the orchidaceae seed-fungus embedding body;

[0048] 4) The orchidaceous plant seed-fungus embedding body described in step 3) is dropped into the calcium chloride aqueous solution to carry out ion exchange reaction, and the orchidaceous plant seed-fungal symbiotic germination complex is obtained.

[0049] The present invention prepares the hyphae of symbiotic fungi into fungal mycelium suspension.

[0050] In the present invention, th...

Embodiment 1

[0064] Preparation method of symbiotic germination complex between Dendrobium officinale seeds and Sebacinales LQ

[0065] 1. Disinfection of Dendrobium officinale seeds: Take the seeds out of the seed bank stored at -20°C and place them at room temperature for 10 hours to bring the temperature of the seeds back to room temperature; put the seeds in a 10ml disposable In the syringe, seal it with a non-woven cloth; draw 8ml of sodium hypochlorite (NaClO) solution with a concentration of 1% (w / v), shake it to fully contact the seeds, and after 5 minutes, draw sterile water to rinse the seeds 4 to 5 times to obtain sterilized seed.

[0066] 2. Preparation of seed-fungus mixed suspension.

[0067] 2.1 Strain activation: Take out the wax-shelled Auriculares strain Sebacinales LQ stored in the slant of the test tube at 4°C, inoculate it on the PDA plate medium, and place it in an artificial climate box at 25±2°C for activation. After about 7 days, wait for The fungal hyphae were t...

Embodiment 2

[0075] 1. Sowing and germination of Dendrobium officinale seed-fungus symbiotic germination complex

[0076] The Dendrobium officinale seed-fungus symbiotic germination complex prepared in Example 1 was sown.

[0077] 1.1 Design two groups of germination experiments of Dendrobium officinale seed-fungus symbiotic germination complex, and conduct seeding experiments on oat agar medium (OMA) and sterile mixed substrates. Divide the sterilized oat agar medium into Petri dishes and the sterilized mixed matrix into disposable lunch boxes.

[0078] 1.2 The Dendrobium candidum symbiotic germination complex is sown on the oat agar medium (OMA), 20 grains are sown in each petri dish, 22 repetitions are set respectively, and 440 grains are sown in total;

[0079] 1.3 Simultaneously sow the Dendrobium officinale seed-fungus symbiotic germination complex on the sterilized mixed substrate, sow 60 grains in each lunch box, set 12 repetitions respectively, and sow 720 grains in total;

[00...

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Abstract

The invention provides an orchid seed-fungus symbiotic germination complex as well as a preparation method and application thereof, and belongs to the technical field of biology. The preparation method of the orchid seed-fungus symbiotic germination complex comprises the following steps of preparing a seed-fungus mixed suspension from seeds and fungus mycelia, dripping the suspension into a sodium alginate aqueous solution to obtain an embedded body, and hardening the embedded body to obtain the seed-fungus symbiotic germination complex; the complex is spherical, and each complex contains 5-10 seeds, after the complex is sown for about 30 days, seeds germinate and start to form seedlings, so that the complex has higher germination rate and seedling survival rate, can be directly applied to sowing and large-scale centralized seedling raising under natural conditions, and the orchid seed-fungus symbiotic germination complex as well as preparation method and application thereof have the advantages of simple manufacturing process, low cost, commercialized production, convenience in storage, transportation and use, high seed germination rate, high seedling formation speed and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an orchid plant seed-fungus symbiotic germination complex and a preparation method and application thereof. Background technique [0002] Orchidaceae (Orchidaceae) is one of the largest families of angiosperms, with nearly 800 genera and more than 28,000 species, but it is also the most threatened plant group in the world. China is one of the countries with the richest species of orchids, with more than 1,700 species, but nearly one-third of the species are endangered due to long-term over-collection due to their high ornamental or medicinal value. Dendrobium (Dendrobium) is the second largest genus of Orchidaceae. There are about 1500-1600 species in the world, all of which are epiphytes, mainly distributed in tropical Southeast Asia and Oceania. There are more than 80 species of Dendrobium in my country, nearly half of which are used in traditional Chinese medicinal ma...

Claims

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Application Information

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IPC IPC(8): A01C1/06A01G22/63C12N11/10C12N11/04C12R1/645
CPCA01C1/06A01G22/63C12N11/10C12N11/04
Inventor 高江云杨华王新菊
Owner YUNNAN UNIV
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