Haematococcus pluvialis culture medium, preparation method thereof and haematococcus pluvialis culture method
A technology of Haematococcus pluvialis and a culturing method is applied in the field of Haematococcus pluvialis culture medium and its preparation and Haematococcus pluvialis cultivation, which can solve the problem that the output of astaxanthin needs to be further improved, and achieves environmentally friendly and sustainable development. Sustained, cost-effective, widely distributed effects
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[0050] Embodiment 1 utilizes seaweed extract and bacterial liquid to promote the method of Haematococcus pluvialis biomass and astaxanthin production
[0051] Embodiments of the present invention include the following steps:
[0052] 1. Cell culture of Haematococcus pluvialis
[0053] (1) Preparation of Gracilaria seaweed extract:
[0054] Gracilaria tenuistipitata and Gracilaria edulis used in this example are Gracilaria tenuistipitata. Take fresh algae of the genus Gracilaria, mix and grind them with tap water at a ratio of 1:20, filter through gauze, and obtain a liquid that is boiled and cooled to obtain the extract of Gracilaria.
[0055] (2) Preparation of Haematococcus pluvialis culture medium:
[0056] Add Gracilaria extract in basal medium (addition concentration is Gracilaria extract: Haematococcus pluvialis basal culture solution=1:500), the composition of basal medium is as in Table 1:
[0057] Table 1 BG11 liquid medium formula
[0058] name Additio...
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