Preparation method and application of polypeptide-containing sheep placenta zymolyte

A peptide sheep fetal enzyme, sheep fetal enzyme technology, applied in application, food science, drug combination and other directions, can solve the problems of large consumption of raw materials, high technical requirements and production costs, long production cycle and other problems, and achieves simple process flow, The effect of improving menopausal syndrome and improving sleep

Inactive Publication Date: 2021-10-01
成都恒福禧生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method has a long production cycle, a large consumption of raw materials, very high t...

Method used

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  • Preparation method and application of polypeptide-containing sheep placenta zymolyte
  • Preparation method and application of polypeptide-containing sheep placenta zymolyte
  • Preparation method and application of polypeptide-containing sheep placenta zymolyte

Examples

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Embodiment 1

[0034] Embodiment 1, the preparation of sheep fetal hydrolyzate of the present invention

[0035] Add 100 grams of sheep fetus freeze-dried powder, add 1000 ml of water, heat to 40°C, adjust the pH to 7.5 with 2mol / L sodium hydroxide, add 2g of trypsin, and react for 2 hours; then heat up to 55°C, and then use 2mol / L / L sodium hydroxide to adjust the pH value to 8, add 2 grams of alkaline protease to react for 2 hours under constant temperature stirring; then add 2 grams of flavor protease to react for 2 hours; then raise the temperature to 90°C, and react at constant temperature for half an hour to inactivate the enzyme ,filter. The supernatant is concentrated under reduced pressure at a vacuum degree of 0.06-0.10Mpa and a temperature of 40-65°C, and then freeze-dried at a vacuum degree of 0.06-0.10Mpa and a temperature of -50-40°C to obtain a solid sheep fetal polypeptide dry powder. Rate 60%.

[0036] After determination, wherein: the polypeptide content is 61.8% (mass ra...

Embodiment 2

[0037] Embodiment 2, the preparation of sheep fetal hydrolyzate of the present invention

[0038] Add 100 grams of sheep fetus freeze-dried powder, add 1000 ml of water, heat to 40 ° C, adjust the pH value to 7.5 with 2 mol / L sodium hydroxide, add 0.5 g of trypsin, and react for 2 hours; heat up to 55 ° C, and then use 2 mol / L sodium hydroxide to adjust the pH value to 8, add 0.5 g of alkaline protease to react for 2 hours under constant temperature stirring; then add 0.2 g of flavor protease to react for 2 hours; then raise the temperature to 90°C, and react at constant temperature for half an hour to inactivate the enzyme ,filter. The supernatant was spray-dried (at an inlet temperature of 180° C. and an outlet temperature of 85° C.) to obtain solid sheep fetal polypeptide dry powder with a yield of 26%.

[0039] After determination, wherein: the polypeptide content is 62.6% (mass ratio), the polysaccharide content is 8.1% (mass ratio); the flavonoid content is 1.9% (mass ...

Embodiment 3

[0040] Embodiment 3, the preparation of sheep fetal hydrolyzate of the present invention

[0041] Take 500 grams of fresh sheep embryos, beat them, add water to 1000 ml, heat to 55 ° C, adjust the pH value to 8 with 2 mol / L sodium hydroxide, add 1 gram of alkaline protease to react for 2 hours under constant temperature stirring; then add 1 gram of The flavor protease was reacted for 2 hours; then the temperature was raised to 90°C, and the reaction was carried out at a constant temperature for half an hour to inactivate the enzyme, and filtered. The supernatant was spray-dried (inlet temperature 180°C, outlet temperature 85°C) to obtain solid sheep fetal polypeptide dry powder. Yield 48%.

[0042] After determination, wherein: the polypeptide content is 71.3% (mass ratio), the polysaccharide content is 8.9% (mass ratio); the flavonoid content is 2.96% (mass ratio).

[0043] The beneficial effects of the present invention will be further illustrated by means of test examples...

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Abstract

The invention discloses a preparation method and application of a polypeptide-containing sheep placenta zymolyte, and belongs to the technical field of enzyme engineering. The method mainly comprises the following steps: (1) taking sheep embryo homogenate or dry powder, adding water and conducting stirring; (2) conducting heating to 50-60 DEG C, adjusting the pH value to 8-9, adding alkaline protease to react for 0.5-12 hours, and then adding flavourzyme to react for 0.5-12 hours; and (3) heating the enzymatic hydrolysate obtained in the step (2) to 80-100 DEG C, conducting reacting for 25-45 minutes, conducting filtering, and drying the filtrate, thereby obtaining the product. The method disclosed by the invention is simple in process, low in raw material consumption and suitable for industrial production of the sheep placenta zymolyte; and the product obtained by the invention has good functions of improving sleep, improving climacteric syndrome and the like, and has a good market prospect.

Description

technical field [0001] The invention belongs to the technical field of enzyme engineering, and in particular relates to a preparation method and application of a polypeptide-containing sheep fetal enzyme hydrolyzate. Background technique [0002] Sheep fetus has always been regarded as a great tonic. Integrating quick healing and nourishing essence, it has the effects of nourishing blood and calming the nerves, plumping the skin, prolonging life, etc. It has been regarded as a treasure since ancient times. The so-called sheep fetus in traditional Chinese medicine refers to the amniotic fluid embryo taken from the abdomen of a ewe who has been pregnant for 3 to 4 months. Because it contains a variety of biological components that are beneficial to the human body, it is more beneficial than the postpartum sheep placenta. High in nutrition, Chinese medicine has always used it as a tonic for the human body. [0003] Modern medical research has confirmed that sheep fetus is ric...

Claims

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Application Information

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IPC IPC(8): A23L33/00A61K35/50A61K35/54A61P15/12A61P25/20
CPCA23L33/00A61K35/50A61K35/54A61P25/20A61P15/12A23V2002/00A23V2200/30A23V2200/322
Inventor 史宗洁张涛朱筱莉张思良周洪云
Owner 成都恒福禧生物技术有限公司
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