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Crocus sativus endophytic fungus and application thereof in control of corm rot

A technology of endophytic fungus and bulb rot, which is applied in the field of plant disease prevention and control, can solve the problem of bulb rot that has not been reported, and achieve the effect of alleviating the disease, broad application prospects, and obvious effects

Active Publication Date: 2021-09-28
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is no report on the application of controlling bulb rot by saffron endophytic fungi

Method used

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  • Crocus sativus endophytic fungus and application thereof in control of corm rot
  • Crocus sativus endophytic fungus and application thereof in control of corm rot
  • Crocus sativus endophytic fungus and application thereof in control of corm rot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The endophytic fungus is isolated and obtained according to the following steps: fresh saffron plants are taken and washed under clear water to remove surface impurities. Cut tissue parts of appropriate size (terminal buds, bulbs, flowers, leaves, and membranous coating), wrap them in gauze and perform three-step surface disinfection treatment, 75% ethanol solution for 1 min, 1% sodium hypochlorite solution for 3 min, and 75% ethanol solution for 30 s. Sterilize and take out, cut out small pieces about 0.5cm×0.5cm in size from the middle part of the tissue and inoculate them on culture plates, 4 pieces per plate. Seal with parafilm, record the number, and incubate at room temperature for 7-14 days. Regularly observe the growth of the colony, and screen out a single target colony according to the shape and color of the colony. Under sterile conditions, pick the tissue pieces from the tip of the target hyphae and transfer them to a freshly prepared PDA petri dish for re-...

Embodiment 2

[0031] Take the cryopreservation tube of CS05 strain, under aseptic conditions, pick a little mycelium with an inoculation needle and place it in the freshly prepared PDA medium for activation, and cultivate it in the shade at 28°C until it matures; take the saffron rot pathogen Fusarium oxysporum F .oxysporum, use a fungal puncher to punch the mature colony into a 0.5cm×0.5cm fungus cake, place the pathogenic bacteria at the center, draw a line at an angle of 120°, inoculate CS05 to a position 2cm away from the center, and carry out plate confrontation test. The results showed that the endophytic fungus CS05 could effectively inhibit the growth of the saffron rot pathogen Fusarium oxysporum in vitro, with a relative inhibition rate of 76.53%, which was higher than that of Trichoderma harzianum, such as Image 6 shown.

[0032] Take the cryopreservation tube of CS05 strain, under aseptic conditions, pick a little mycelium with an inoculation needle and place it in the freshly...

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Abstract

The invention discloses a crocus sativus endophytic fungus and an application thereof in control of corm rot, and belongs to the technical field of plant disease prevention and control. The endophytic fungus is separated from crocus sativus living bodies of plants ofcrocus sativus, iridaceae by adopting an endophytic fungi separation and purification technology, belongs to trametes fungi, polyporales, polyporaceae, and is classified and named as trametes versicolor CS05. The preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 21948. The invention also discloses an application of the endophytic fungus: the endophytic fungus can inhibit the hypha growth of the corm rot pathogenic bacteria Fusarium oxysporum of the crocus sativus, the relative inhibition rate is 76.53%, meanwhile, the condition of the corm rot of the crocus sativus is effectively relieved, and the condition index is reduced by 81.13%. The endophytic fungus provided by the invention has an obvious effect of controlling the corm rot of the crocus sativus, and brings a wide application prospect to the field of biological control of crocus sativus fungus diseases.

Description

technical field [0001] The invention relates to the technical field of plant disease prevention and control, in particular to an endophytic fungus of saffron and its application in the prevention and treatment of bulb rot. Background technique [0002] Saffron is the dry stigma of Crocus Sativus L., a plant of the genus Crocus Iridaceae. It is one of the famous authentic medicinal materials "Xinzhe Bawei" in Zhejiang Province. The whole plant only has the three-branched red filamentous stigma of the pistil It can be used as medicine, with low yield and high value. It is called "plant gold" and has the effects of promoting blood circulation, removing blood stasis, cooling blood and detoxification, relieving stagnation and calming the nerves. [0003] Bulb rot is the main disease in the cultivation of saffron. High temperature and high humidity are conducive to the occurrence of the disease. During the high temperature in summer from late June to late August, it can cause seri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/30A01P3/00C12R1/645
CPCC12N1/14A01N63/30
Inventor 朱波秦路平陆洁淼张家豪
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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