Novel coronavirus pneumonia recombinant human type 5 adenovirus vaccine

A technology of adenovirus and virus strains, applied in the direction of viruses, virus peptides, antiviral agents, etc., can solve problems such as economic losses

Active Publication Date: 2021-09-28
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mink is a special economic animal. Once infected with new coronary pneumonia, it will be culled on a large scale to prevent its spread, which will cause huge economic losses.

Method used

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  • Novel coronavirus pneumonia recombinant human type 5 adenovirus vaccine
  • Novel coronavirus pneumonia recombinant human type 5 adenovirus vaccine
  • Novel coronavirus pneumonia recombinant human type 5 adenovirus vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1: Materials and methods

[0054] 1 Materials and methods

[0055] 1.1 Virus strains

[0056] rAd-tPASopti, a recombinant human adenovirus strain rAd-tPASopti expressing SARS-CoV-2S protein optimized by mammalian codon bias, was constructed by the important zoonosis and severe exotic disease innovation team of Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, save. The SARS-CoV-2 isolate HRB25 and the SARS-CoV-2 mouse-adapted strain HRB26M were isolated, identified and preserved by the important zoonoses and severe exotic diseases innovation team of Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences.

[0057] 1.2 cells

[0058] Vero E6 cells (ATCC, No.CRL-1586) were preserved and cultured by the important zoonosis and severe exotic disease innovation team of Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences. The 293A cells expressing human adenovirus type 5 E1 protein were...

Embodiment 2

[0084] Embodiment 2: experimental result

[0085] 2.1 Construction and rescue of recombinant adenovirus expressing tPASopti6PB protein gene

[0086] The PCR method was used to amplify the tPASopti6PB protein gene with pBlue-tPASopti6PB as a template and primers pShuttle-CMV-F and pShuttle-CMV-R. The tPASopti6PB protein gene was cloned into the KpnI and XhoI sites of the shuttle vector pShuttle-CMV by enzyme digestion and ligation to construct the recombinant shuttle vector pShuttle-CMV-tPASopti6PB containing the tPASopti6PB protein gene. The results of enzyme digestion and sequence determination showed that the tPASopti6PB protein gene was successfully cloned into the pShuttle-CMV vector. The shuttle vector pShuttle-CMV-tPASopti6PB plasmid linearized by the restriction endonuclease PmeⅠ was transformed into Escherichia coli BJ5183-AD-1 competent cells, the tPASopti6PB protein gene was homologously recombined into the pAdEasy-1 vector, the genomic DNA was extracted, and the sp...

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Abstract

The invention provides a vaccine composition containing a modified SARS-CoV-2 spike protein. The vaccine composition is characterized in that a replication-deficient human type 5 adenovirus rAd virus strain is combined with a novel modified SARS-CoV-2 spike protein (S6P) or an immunogenic derivative thereof to construct a recombinant replication-deficient human type 5 adenovirus for preventing infection of a novel coronavirus, especially against novel coronavirus infection in mammals.

Description

technical field [0001] The present invention relates to a preventive vaccine composition and method for mammalian novel coronavirus pneumonia, and further relates to a preventive vaccine composition and method for recombinant human adenovirus type 5 against mammalian novel coronavirus pneumonia. Background technique [0002] Coronavirus disease 2019 (COVID-19) is a highly transmissible zoonotic disease caused by a novel coronavirus (Severe Acute Respiratory Syndrome Coronavirus 2, SARS-CoV-2). COVID-19 is still prevalent in the world, constantly endangering human life and health. As of May 4, 2021, the World Health Organization (WHO) reported a total of more than 150 million confirmed cases and nearly 3.21 million deaths. At present, the traceability of SARS-CoV-2 has not been completed. Preliminary studies suggest that, The natural host of SARS-CoV-2 may be some species of bats, and the intermediate host may involve pangolins, but these hypotheses still need stronger evide...

Claims

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Application Information

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IPC IPC(8): C07K14/165C12N15/861C12N15/50C12N15/62A61K39/215A61P31/14A61P11/00
CPCC07K14/005C12N15/86A61K39/12A61P31/14A61P11/00C12N2710/10043C12N2770/20022C12N2770/20034A61K2039/5256
Inventor 步志高王翀
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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