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Method for detecting 2, 4-dichlorophenoxyacetic acid

A technology of dichlorophenoxyacetic acid and phosphatase, which is applied in the field of pesticide detection, can solve the problems of time-consuming, long-term consumption, survival rate, reproductive ability decline, etc.

Active Publication Date: 2021-09-24
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although 2,4-D is classified as a low-toxic pesticide (rat acute oral half-lethal dose: 639-764mg / kg), studies have found that 2,4-D may have a variety of toxic effects, including carcinogenicity, neurotoxicity , reproductive toxicity, etc.
The derivative of 2,4-D, 2,4-D-butyl ester, because of its "2,4-D-butyl ester is volatile, it is easy to cause phytotoxicity accidents to registered crops and adjacent crops when used; the acute toxicity to aquatic organisms is highly toxic , chronic toxicity manifests as decreased survival, reproductive capacity
However, existing biosensors require time-consuming preparation processes, such as electrode preparation or detection probe synthesis, which are often tedious and time-consuming, and do not match the needs of rapid detection of pesticide residues

Method used

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  • Method for detecting 2, 4-dichlorophenoxyacetic acid
  • Method for detecting 2, 4-dichlorophenoxyacetic acid
  • Method for detecting 2, 4-dichlorophenoxyacetic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Synthesis of cerium-pyrophosphate coordination polymer fluorescent nanoparticles (Ce-ppi for short):

[0053] First, an alkaline phosphatase (ALP for short) solution with a concentration of 100 U / mL was prepared using Tris-HCl buffer (concentration: 50 mM, pH=8.0) as a solvent. 2,4-dichlorophenoxyacetic acid ( Abbreviated as 2,4-D) solution, used to establish a standard curve method for quantitative detection of 2,4-D.

[0054] Take 5 μL of LALP solution in a 2 mL plastic centrifuge tube, then add 25 μL of 2,4-D solution into the centrifuge tube, mix the two thoroughly, and let stand at 37°C for 15 minutes. Afterwards, 10 μL of 1 mM sodium pyrophosphate solution and 20 μL of 50 mM Tris-HCl buffer solution (pH=8.0) were added to the centrifuge tube, mixed thoroughly, and left to react at 37° C. for 25 minutes. After the reaction was completed, 20 μL of 0.5 mM cerium nitrate solution was added to the centrifuge tube, and the total volume of the solution was diluted to 5...

Embodiment 2

[0057] Adopt the method identical with embodiment 1 to investigate the influence of ALP concentration, standing time, temperature of reaction and reaction time on detection result, result is as follows:

[0058] When the ALP concentration reaches 1.25U / mL, increase the ALP concentration again, the fluorescence intensity of Ce-PPi will not change, indicating that the PPi reaction is complete at this time ( image 3 in A). Therefore, 1U / mL was chosen as the ALP concentration.

[0059] After standing time 25min, the fluorescence intensity of Ce-PPi no longer changes ( image 3 in B). Therefore, 25min was chosen as the time for the mixed reaction of ALP and PPi.

[0060] When the reaction temperature was 37 °C, the fluorescence intensity of Ce-PPi reached the lowest value ( image 3 in C). Therefore, 37°C was selected as the reaction temperature.

[0061] from image 3 In D, it can be seen that when the mixing reaction time of 2,4-D and ALP is 15 min, the fluorescence inten...

Embodiment 3

[0063] Test sample test method:

[0064] The ability of the method to detect 2,4-D residues in real samples (tap water, rice and Chinese cabbage) was validated by the addition recovery method. The tap water was taken from the College of Science, China Agricultural University. After passing through a 0.22 μm filter membrane, the 2,4-D standard solution was diluted to prepare water samples containing different concentrations of 2,4-D (0.05, 0.2 or 0.1 mg / L). During detection, 50 μL of water sample containing 2,4-D was taken, and the remaining detection steps were the same as those for detecting 2,4-D in the standard solution (Example 1). Rice and Chinese cabbage samples were purchased from local supermarkets. The extraction method of 2,4-D residues added in the samples refers to the above-mentioned literature reports. The specific steps are as follows: Accurately weigh 1 g of the mashed samples into a 10 mL plastic centrifuge tube, add 10 μL of 2 , 4-D solution (concentrations ...

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Abstract

The invention provides a method for detecting 2, 4-dichlorphenoxyacetic acid and belongs to the technical field of pesticide detection. The method comprises steps of an alkaline phosphatase solution and a sample being mixed and left to stand, and standing liquid being obtained; mixing the standing liquid with a sodium pyrophosphate solution and a Tris-HCl buffer solution, and carrying out a reaction to obtain a reaction liquid; mixing the reaction solution with a cerous nitrate solution, oscillating to obtain an oscillating solution, measuring the fluorescence emission spectrum of the oscillating solution to obtain the maximum fluorescence intensity, and converting the maximum fluorescence intensity into an enzyme inhibition rate; and substituting the enzyme inhibition ratio into the linear equation to obtain the concentration of 2, 4-dichlorophenoxyacetic acid in the sample. By adopting the method provided by the invention, a detection probe does not need to be prepared, and a detection result can be obtained within 45 minutes; the linear range of the linear equation is 2.5-200 [mu] g / L, and the detection limit is 0.98 [mu] g / L.

Description

technical field [0001] The invention belongs to the technical field of pesticide detection, in particular to a method for detecting 2,4-dichlorophenoxyacetic acid. Background technique [0002] 2,4-Dichlorophenoxyacetic acid (2,4-D) is a versatile agrochemical that can be used as a plant growth regulator or herbicide. The biological activity of 2,4-D varies with the concentration: at low concentration, 2,4-D has the activity similar to that of plant endogenous auxin indole acetic acid, which can promote plant growth and prevent fruit drop; while at high concentration Shi 2,4-D has an inactivating effect on broad-leaved weeds. 2,4-D has the advantages of low price and less application amount, and is widely used in agricultural production. Although 2,4-D is classified as a low-toxic pesticide (rat acute oral half-lethal dose: 639-764mg / kg), studies have found that 2,4-D may have a variety of toxic effects, including carcinogenicity, neurotoxicity , reproductive toxicity, et...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/643
Inventor 王鹏王冬伟周志强刘东晖
Owner CHINA AGRI UNIV
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