The method of ibs-d experimental animal model to improve the molding rate and reduce the mortality

Abstract

The invention provides an IBS-D experimental animal model method for improving the molding rate and reducing the mortality rate, which selects adult mice as the modeling experiment object, and includes the following steps: Step 1, colorectal dilation: first, lightly touch the anus of the mouse , make the mouse defecate; then use a tracheal cannula with a balloon to insert it into the mouse anus, wherein the balloon end of the tracheal cannula is located in the mouse anus, and the outer end of the tracheal cannula is fixed at the root of the mouse tail; then the mouse is placed in a transparent observation Indoors, the transparent observation room can limit the left and right movement of the mouse; after the mouse is calm, inject gas into the balloon of the tracheal intubation, so that the balloon has a certain expansion pressure, and stimulate the colorectum of the mouse; Step 2, chronic restraint stress: the mouse The transparent observation room was taken out, and the abdomen and limbs of the mice were immobilized in a supine position. The invention can better completely replicate the disease characteristics, at the same time, it can reduce the mortality rate and improve the molding rate; meanwhile, it can make the modeling effect more durable and stable.

Description

technical field [0001] The invention relates to the technical field of medical research, and relates to a method for establishing an animal model, in particular to an IBS-D (irritable bowel syndrome with diarrhoea, diarrhea-type irritable bowel syndrome) experimental animal model method for improving the molding rate and reducing the mortality rate. . Background technique [0002] IBS (irritable bowel syndrome, irritable bowel syndrome) is a common functional bowel disease, mainly characterized by recurrent abdominal pain, accompanied by relief of symptoms after defecation, changes in defecation frequency, and abnormal stool properties. The incidence of IBS is high, with a global prevalence of about 5% to 15%, the lowest prevalence in Southeast Asia (7.0%), and the highest in South America (21.0%). Women are more prevalent than men, and are more likely to develop the disease in chronically high-stress environments or childhood trauma. IBS is divided into diarrhea-predomina...

AI Technical Summary

Problems solved by technology

[0004] The pathogenesis of IBS is complex and diverse. Gastrointestinal motility and paresthesia are its pathophysiological basis. Its pathogenesis is related to the change of brain-gut axis function. It is a biological-psychological-social disorder. Therefore, it is difficult to establish an animal model.

The current modeling methods can construct the visceral sensitivity and abnormal motility characteristics of IBS-D to a certain extent, but they lack the manifestations of emotional disorders such as anxiety and depression, and cannot completely replicate the characteristics of the disease

At the same time, there is also a certain failure rate and mortality rate in the process of modeling

[0005] In summary, most of the existing animal models of irritable bowel syndrome start from a certain aspect of the pathophysiological mechanism, which has limitations to varying degrees, and there is a lack of accurate comparative studies on the advantages and disadvantages of the commonly used model preparation methods

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