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Salvianic acid A sodium self-assembled liposome for treating ulcerative colitis and preparation method thereof

A technology of ulcerative colitis and danshensu sodium, applied in the field of medicine, to achieve the effect of improving the imbalance of intestinal flora, improving clinical symptoms and good treatment

Pending Publication Date: 2021-09-17
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] By consulting domestic and foreign literature and patents, no domestic and foreign researchers have combined Danshensu Sodium and self-assembled liposome technology for the treatment of ulcerative colitis

Method used

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  • Salvianic acid A sodium self-assembled liposome for treating ulcerative colitis and preparation method thereof
  • Salvianic acid A sodium self-assembled liposome for treating ulcerative colitis and preparation method thereof
  • Salvianic acid A sodium self-assembled liposome for treating ulcerative colitis and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] a. Take 40 mg of phospholipids and 20 mg of cholesterol, add them to 10 mL of chloroform to dissolve, and then recover and remove the organic solvent chloroform by rotary evaporation under reduced pressure to form a thin film, add 6 mL of ether to dissolve as an organic phase.

[0043] b. Dissolve 6 mg of danshensu sodium in 2 mL of purified water as the water phase.

[0044] c. Add the aqueous phase obtained in step b to the organic phase obtained in step a, and use a cell disruptor to sonicate in an ice bath at 0°C. The method is to ultrasonicate for 3s, stop for 5s, ultrasonic power 200W, and ultrasonically for 10min to form a uniform emulsion. .

[0045] d. Evaporate the emulsion obtained in step c under reduced pressure at room temperature, evaporate the organic solvent, add an appropriate amount of purified water dropwise after reaching a colloidal state, add purified water to 4 mL, continue to evaporate under reduced pressure for hydration, and use 0.45 and 0.22 ...

Embodiment 2

[0049] a. Take 50 mg of phospholipids and 10 mg of cholesterol, add them into 10 mL of chloroform to dissolve, recover under reduced pressure to remove the organic phase, add 6 mL of ether to dissolve as the organic phase.

[0050] b. Dissolve 6 mg of danshensu sodium in 2 mL of purified water as the water phase.

[0051] c. Add the aqueous phase obtained in step b to the organic phase obtained in step a, and use a cell disruptor to sonicate in an ice bath. The method is to ultrasonicate for 3s, stop for 5s, and ultrasonic power 200W for a total of 10min to form a uniform emulsion.

[0052] d. Evaporate the emulsion obtained in step c under reduced pressure at room temperature, evaporate the organic solvent, add an appropriate amount of purified water dropwise after reaching a colloidal state, add purified water to 4 mL, continue to evaporate under reduced pressure for hydration, and use 0.45 and 0.22 μm microporous membrane to obtain liposomes.

[0053] e. Slowly add the lip...

Embodiment 3

[0056] a. Take 48 mg of phospholipids and 12 mg of cholesterol and add them into 5 mL of chloroform as the organic phase.

[0057] b. Dissolve 6 mg of danshensu sodium in 2 mL of purified water as the water phase.

[0058] c. Add the aqueous phase obtained in step b to the organic phase obtained in step a, and use a cell disruptor to sonicate in an ice bath. The method is to ultrasonicate for 3s, stop for 5s, and ultrasonic power 200W for a total of 10min to form a uniform emulsion.

[0059] d. Evaporate the emulsion obtained in step c under reduced pressure at room temperature, evaporate the organic solvent, add an appropriate amount of purified water dropwise after reaching a colloidal state, add purified water to 4 mL, continue to evaporate under reduced pressure for hydration, and use 0.45 and 0.22 μm microporous membrane to obtain liposomes.

[0060] e. Slowly add the liposomes prepared in step d to an equal volume of CH solution (0.2%, v / w) dropwise under stirring, cont...

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Abstract

The invention belongs to the technical field of medicines, and particularly relates to a salvianic acid A sodium oral self-assembled liposome for treating ulcerative colitis and a preparation method thereof. The salvianic acid A sodium oral self-assembled liposome for treating ulcerative colitis prepared by the method disclosed by the invention is prepared by taking salvianic acid A sodium, phospholipid, cholesterol, chitosan and sodium alginate as raw materials and adopting a reversed-phase evaporation method and an LbL self-assembly technology. A mouse experiment of the DSS (dextran sulfate sodium) induced ulcerative colitis proves that the oral salvianic acid A sodium self-assembled liposome prepared by the invention has the effects of improving clinical symptoms of mice with colitis, reducing the content of proinflammatory factors TNF-alpha, IF-6 and IF-1beta in serum of the mice with ulcerative colitis, increasing the content of anti-inflammatory factor IL-10 and alleviating intestinal flora imbalance of mice with ulcerative colitis, thereby having a relatively good medicine effect on treating ulcerative colitis.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to an oral self-assembled liposome of danshensu sodium for treating ulcerative colitis and a preparation method thereof. Background technique [0002] Inflammatory bowel disease (IBD), is a chronic and progressive inflammatory disease of the gastrointestinal tract, including ulcerative colitis (UC) and Crohn's disease (CD). At present, the incidence of inflammatory bowel disease has always been high in the world, especially in Asia, showing an upward trend, and the incidence in some countries has increased by two to three times. Among them, ulcerative colitis is a common and refractory disease. The current pathogenesis is not completely clear. It is generally believed that the etiology may be related to factors such as genetics, intestinal flora imbalance, and environment (Ingrid Ordás, Lars Eckmann, Mark Talamini, Daniel C Baumgart, & William J Sandborn. (2012). Ulcer...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K47/36A61K31/192A61P1/00A61P1/04A61P29/00
CPCA61K9/1277A61K9/1272A61K9/0053A61K47/36A61K31/192A61P1/00A61P1/04A61P29/00
Inventor 唐岚曹李鹏傅璐璐张振海单伟光欧志敏
Owner ZHEJIANG UNIV OF TECH
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