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Wild tetrasyna mongolica maxim callus induction culture medium and induction method

A technology of callus induction and quadruple wood, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of inability to raise mercury, disinfection, etc.

Active Publication Date: 2021-09-17
乌海市泽汇园林绿化工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during this experiment period, mercuric acid was listed as a banned drug, so it cannot be used for disinfection

Method used

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  • Wild tetrasyna mongolica maxim callus induction culture medium and induction method
  • Wild tetrasyna mongolica maxim callus induction culture medium and induction method
  • Wild tetrasyna mongolica maxim callus induction culture medium and induction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] This embodiment first analyzes the disinfection of wild quadrangle wood:

[0051] (1) Test the disinfection effect of 75% alcohol and 10% NaClO solution

[0052] Select an appropriate amount of quadrangle wood stems, keep the leaves above, and clean the surface of the stems with a small brush. Use a scalpel to cut into about 3cm in size, put it in a beaker, rinse it with tap water for about 30 minutes, and wash it with sterile water 3 times.

[0053] Put the medium, 75% alcohol solution, 10% sodium hypochlorite solution and other operating supplies into the ultra-clean workbench for ultraviolet disinfection for 20 minutes.

[0054] The stem section of Sihe wood was treated with 75% alcohol solution for 6 minutes, and washed 3 times with sterile water. Then soak in 10% NaClO solution for 10 minutes, wash thoroughly with sterile water several times, and transfer to the culture medium (in this embodiment, the IMA content of the culture medium is 3 mg / l).

[0055] (2) Test...

Embodiment 2

[0085] Under the premise of the preferred disinfection formula and disinfection process obtained in the examples, configure 1 / 2 MS medium. On the basis of Table 2, 3 bottles of hormone IBA were added at a concentration of 1ml / L, 2ml / L, 3ml / L, and 5ml / L respectively, totaling 9 bottles.

[0086] The sterilized Sihemu explant material was moved into a culture bottle, culture conditions: temperature: 22° C., air humidity: 11%, light culture.

[0087] Result analysis:

[0088] In order to induce the callus of Tetragalus, the young shoots of Tetraphyllum were used as materials, and different concentrations of IBA hormones were added to the 1 / 2MS medium to induce the explants of Tetraphyllum explants. The experimental results are as follows image 3 .

[0089] Depend on image 3 It can be seen that in the treatment of hormone IBA1 mg / L, 2mg / L, 3mg / L, and 5mg / L, the callus that appeared in the culture flask using 3mg / LIBA was the most obvious.

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Abstract

The invention discloses a wild tetrasyna mongolica maxim callus induction culture medium. The wild tetrasyna mongolica maxim callus induction culture medium comprises 1 / 2 MS salt, MES, indolebutyric acid IBA, cane sugar and agar, the final concentrations of the 1 / 2 MS salt, the MES, the indolebutyric acid IBA, the cane sugar and the agar in the culture medium are 2.3 g / l, 0.25 g / l, 3 mg / l, 10 g / l and 7 g / l, and KOH is added to adjust the pH to 5.75-5.85.The invention further discloses an induction culture method for the callus of the wild tetrasyna mongolica maxim. The induction culture method comprises the following steps of by taking tetrasyna mongolica maxim young branches as explant materials, disinfecting the tetrasyna mongolica maxim young branches with 75% alcohol for 1 minute, treating the tetrasyna mongolica maxim young branches with a 10% sodium hypochlorite solution for 16 minutes, treating the tetrasyna mongolica maxim young branches with a 30% hydrogen peroxide solution for 10 minutes, transferring the tetrasyna mongolica maxim young branches into a culture medium, and inducing tetrasyna mongolica maxim explant callus with 1 / 2MS + 3mg / L IBA under the culture conditions of 16h illumination and 8h darkness; the callus disinfection and the culture medium callus culture are carried out in the disinfection mode, so that ideal calluses can be obtained, and certain help can be provided for rescuing similar endangered species such as tetrastigma mongolicum maxim.

Description

Technical field: [0001] The invention relates to a quadrangle tree callus induction medium and an induction method, belonging to the field of plant cultivation. Background technique: [0002] Tetraenamongolica Maxim is a dwarf shrub with succulent leaves belonging to the genus Tetraenamongolica in the family Zygophyllaceae. According to the research, Sihemu originated from the tropical region of the southern continent of Mongolia. It is a remnant plant before the Tertiary Period 70 million years ago. It is now on the verge of extinction and is known as the "giant panda" of the plant kingdom. At present, wild Tetragalus only exists in the northwest of the Ordos Plateau in the Inner Mongolia Autonomous Region of my country, south of the Kubuqi Desert, and the foothills of Zhuozi Mountain, and a small amount extends to the adjacent Uda low mountain remnants. The root system of quadrangle wood is well developed, which can effectively prevent wind and sand fixation and reduce so...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/00
Inventor 李兴朝贾建国徐军王海军康瑞张艳霞王杨王瑞刚
Owner 乌海市泽汇园林绿化工程有限公司
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