Gene sequence with molecular switch, plasmid and immune cell

Abstract

The invention discloses a gene sequence with a molecular switch, a plasmid and an immune cell, and belongs to the technical field of molecular biology. The gene sequence comprises an excitation component, a connection region and a response component, wherein the excitation component comprises a target binding region and a transcription regulation region; the response component comprises an expression region and a promoter region matched with the transcription regulation region; the target binding region of the excitation component is used for expressing a target binding receptor; the transcription regulation region is used for expressing a transcription regulation factor; after the target binding receptor is used for being bound with a target, the transcription regulation factor is separated from the target binding receptor, is bound to the promoter region, and activates a promoter to start expression of the gene sequence in the expression region; and the connection region connects the excitation component and the response component in the same gene sequence. The preparation flow is simplified, the transfection efficiency is improved, and industrial preparation of virus particles and CAR cells is facilitated.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a gene sequence, a plasmid and an immune cell with a molecular switch. Background technique [0002] With the continuous deterioration of global environmental factors, the incidence and mortality of malignant tumors are on the rise, regardless of developed or developing countries, and malignant tumors have become the greatest threat to human life and health. [0003] Chimeric antigen receptor immune cells are a new type of precisely targeted immune cells that kill tumors. In recent years, through optimization and improvement, they have achieved good results in tumor killing. They are very promising, accurate, fast and efficient Way. Chimeric antigen receptor immune cells (CAR cells) include CAR-T, CAR-NK, CAR-B and CAR-MP (CAR-macrophage, CAR-macrophage), which is a chimeric antigen receptor ( Chimeric Antigen Receptor, CAR)-based immune cells. Through in vitro gene ...

AI Technical Summary

Problems solved by technology

As a result, CAR-T cells are always in an activated state, and CAR-T is prone to exhaustion, anergy, and persistence (persistence), and because of no tissue specificity, on-target / off-tumor side effects are not possible avoid

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