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Antibacterial lactobacillus curvatus capable of producing exopolysaccharides and application thereof

A technology of Lactobacillus campylobacter and extracellular polysaccharide, applied in the field of microorganisms, can solve problems such as broad-spectrum bacteriostasis and exopolysaccharide that do not involve Lactobacillus campylobacter

Active Publication Date: 2021-09-10
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The researches reported so far are mainly focused on the probiotic function and bacteriocin produced by Lactobacillus campylobacter, but not on the broad-spectrum antibacterial and exopolysaccharide of Lactobacillus curvedus

Method used

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  • Antibacterial lactobacillus curvatus capable of producing exopolysaccharides and application thereof
  • Antibacterial lactobacillus curvatus capable of producing exopolysaccharides and application thereof
  • Antibacterial lactobacillus curvatus capable of producing exopolysaccharides and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1: Isolation, screening and identification of Lactobacillus curvatus SJTUF 62116

[0057] 1. Isolation of Lactobacillus curvatus SJTUF 62116

[0058] The strain was isolated from naked carp in Qinghai Lake, China. Aseptically collect the viscera of the naked carp and store in a refrigerator at 4°C. Under aseptic conditions, take 5 g of the sample and add it to 45 mL of sterile water, homogenize for 5 min, and then serially dilute (10 -1 -10 -5 ) and shake evenly. Take 100 μL of dilutions of different gradients, spread them on MRS solid medium, and culture them anaerobically at 30°C for 48h. Observe the colonies formed on each plate, pick a single colony to streak on the MRS solid medium plate, and culture anaerobically at 30°C for 48h.

[0059] 2. Screening of Lactobacillus curvatus SJTUF 62116

[0060] Pick a single colony into the MRS liquid medium, and culture it statically at 30°C for 24h. The fermentation broth was centrifuged and filtered with a 0.22...

Embodiment 2

[0069] Example 2: Evaluation of probiotic function of Lactobacillus curvatus (Lactobacillus curvatus) SJTUF 62116

[0070] 1. Evaluation of acid-base adaptability

[0071] The strains were inoculated into MRS liquid medium with a pH of 3.0, 4.0, 9.0 and 10.0, cultured statically at 30°C for 7 days, and MRS liquid medium with the same pH was used as a control, and the results were observed and recorded. The results showed that the strain could grow well under the environment of pH 3.0, 4.0, 9.0 and 10.0, which indicated that it had strong adaptability to acid and alkali.

[0072] 2. Evaluation of adaptability to cold and heat

[0073] The strains were inoculated into MRS liquid medium, cultured statically at 5°C and 10°C for 14 days, and at 45°C and 50°C for 7 days, and MRS liquid medium was used as a control, and the results were observed and recorded. The results showed that the strain could grow well at the temperature of 5°C, 10°C, 45°C and 50°C, which indicated that it h...

Embodiment 3

[0107] Example 3: Extraction and purification of exopolysaccharide from Lactobacillus curvatus SJTUF 62116

[0108] The twice-activated strains were inoculated in MRS liquid medium at 2% (v / v), and cultured statically at 30° C. for 24 hours. The fermented liquid was heated in boiling water for 10 min and then cooled to room temperature. Subsequently, 80% trichloroacetic acid solution was added to a final concentration of 4% (w / v), and stirred at room temperature for 2 h. The supernatant was collected by centrifugation (12 000 g, 15 min, 4° C.), and concentrated under reduced pressure to one-third of the original volume. Add 3 times the volume of absolute ethanol to the concentrated solution, let stand at 4°C for 12h, and then centrifuge (12000g, 20min, 4°C) to collect the precipitate. The precipitate was dissolved in distilled water, concentrated under reduced pressure, and then transferred to a dialysis bag with a molecular weight cut-off of 14KDa for dialysis for 2 days, a...

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Abstract

The invention discloses antibacterial lactobacillus curvatus capable of producing exopolysaccharides and application thereof, and relates to the field of microorganisms. The preservation number of the lactobacillus curvatus SJTUF 62116 provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No.21575. The lactobacillus curvatus has very strong adaptability to acid, alkali and temperature, and can tolerate a simulated gastrointestinal tract environment; and the lactobacillus curvatus and the fermentation supernatant thereof have the capabilities of inhibiting pathogenic bacteria and resisting oxidation. In addition, the lactobacillus curvatus can produce the exopolysaccharides, and the exopolysaccharides obtained by fermenting, centrifuging and precipitating the lactobacillus curvatus and neutral components of the exopolysaccharides obtained by purifying have the capabilities of inhibiting pathogenic bacteria and resisting oxidation. According to the present invention, the lactobacillus curvatus SJTUF 62116 obtained through separation not only has the broad-spectrum antibacterial function, but also can produce the exopolysaccharides with the biological activity, such that the Lactobacillus curvatus SJTUF 62116 can be widely used in the antibacterial and anti-oxidation fields.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a lactobacillus campylobacter capable of tolerant to simulated gastrointestinal tract environment, antibacterial and producing extracellular polysaccharide and application thereof. Background technique [0002] Lactic acid bacteria, as one of the important probiotics, play a positive role in human health, and they are generally recognized as safe (generally recognized as safe, GRAS). The probiotic effect of lactic acid bacteria is manifested in regulating immunity, reducing serum cholesterol levels, preventing cardiovascular diseases, maintaining the balance of intestinal flora, promoting nutrient absorption, alleviating lactose intolerance and inhibiting the formation of tumor cells, etc. Bacteria are inhibited. The probiotic effect of lactic acid bacteria is closely related to its metabolites, such as organic acids, bacteriocins, mannitol, 1,3-propanediol, fatty acids, and exopolysacch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04A61K35/747A61K31/715A61P31/04A61P39/06C12R1/225
CPCC12N1/20C12P19/04A61K35/747A61K31/715A61P31/04A61P39/06Y02A50/30
Inventor 史贤明朱瑾瑜谈重芳
Owner SHANGHAI JIAO TONG UNIV
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