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Method for detecting content of gamma-aminobutyric acid in cosmetics

A technology of aminobutyric acid and detection method, applied in the field of gamma-aminobutyric acid detection, can solve the problems that affect the detection accuracy of gamma-aminobutyric acid, it is difficult to detect low-level samples, and the derivative reaction is susceptible to external interference, etc. , to avoid diffusion and overlap, improve detection sensitivity and accuracy, and achieve high accuracy

Pending Publication Date: 2021-09-03
BLOOMAGE BIOTECHNOLOGY CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Because γ-aminobutyric acid has no ultraviolet absorption, the detection of its content by high performance liquid chromatography requires a reaction with a derivatization reagent to generate a compound with ultraviolet absorption. Separation by the chromatographic column will lead to poor repeatability and low accuracy of the test results, which will affect the detection of γ-aminobutyric acid content
Post-column derivatization cation-exchange chromatography is to derivatize gamma-aminobutyric acid after being separated by a cation-exchange chromatographic column. It has the advantages of fast reaction speed and good stability of derivative products. The commonly used post-column derivatization reagents are indrin However, the ninhydrin derivatization method requires strict sample pretreatment, the operation is more complicated, and the sensitivity is low, so it is difficult to detect low-level samples
The commonly used mobile phase and elution program for the detection and separation of amino acids by amino acid analyzers is used to detect γ-aminobutyric acid. The peak time of γ-aminobutyric acid is around 1.5min, which is easily affected by the solvent peak, and the peak area is very small. , baseline fluctuations can easily affect the accuracy of GABA detection

Method used

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  • Method for detecting content of gamma-aminobutyric acid in cosmetics
  • Method for detecting content of gamma-aminobutyric acid in cosmetics
  • Method for detecting content of gamma-aminobutyric acid in cosmetics

Examples

Experimental program
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Effect test

preparation example Construction

[0041] Preparation of reference substance: use hydrochloric acid to dissolve the γ-aminobutyric acid reference substance, and filter to obtain the reference substance solution of γ-aminobutyric acid;

[0042] Preparation of the test product: use hydrochloric acid to dissolve the cosmetic test product, and filter to obtain the test product solution;

[0043] Preparation of blank solution: after filtration of hydrochloric acid, a blank solution was obtained;

[0044] Determination: use a separation column and a post-column derivatization reaction column to detect the reference solution, the test solution and the blank solution to obtain the content of γ-aminobutyric acid in the cosmetic test product.

[0045] In one embodiment, the concentration of the hydrochloric acid is 0.02mol / L-0.06mol / L, preferably 0.02mol / L.

[0046]For example, the concentration of the hydrochloric acid may be 0.02 mol / L, 0.03 mol / L, 0.04 mol / L, 0.05 mol / L, 0.06 mol / L, and the like.

[0047] The hydroc...

Embodiment 1-1

[0109] 1. Instrument reagents

[0110] LA8080 Amino Acid Analyzer Analytical Balance: METTLER TOLEDO AL104

[0111] Ultrapure water, hydrochloric acid (analytical grade) γ-aminobutyric acid reference substance (99.9%)

[0112] 2. Chromatographic conditions

[0113] Separation column: packed cation exchange resin column (4.4mm×60mm, 5μm model: 852-8534)

[0114] Reaction column: filled with quartz sand (4.6mm×40mm, model: 8L03600)

[0115] Separation flow rate: 0.45ml / min

[0116] Reaction flow rate: 0.3ml / min

[0117] Separation column temperature: 55℃;

[0118] Reaction column temperature: 35°C;

[0119] Injection volume: 20μL;

[0120] Detection wavelength: 336nm

[0121] Gradient elution procedure:

[0122] time / min A％ B％ C％ D％ E％ R1％ R2％ R3％ 0.0 100 -- -- -- -- 50 50 -- 3.5 -- 100 -- -- -- 50 50 -- 3.6 -- -- 100 -- -- 50 50 -- 6.5 -- -- 100 -- -- 50 50 -- 6.6 -- -- 23 77 -- 50 50 -- ...

Embodiment 1-2

[0145] The sample to be tested in Example 1 was replaced with batch number: 210309, and other conditions were the same as those in Example 1. The content of γ-aminobutyric acid in cosmetics was determined. The results are shown in Table 2, and the detection limit was 3 × 10 -4 ppm.

[0146] The detection result of table 2 embodiment 1-2

[0147]

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Abstract

The invention discloses a method for detecting gamma-aminobutyric acid in cosmetics, and the method comprises the following steps: preparation of a reference substance: dissolving the gamma-aminobutyric acid reference substance with hydrochloric acid, and filtering to obtain a reference substance solution of gamma-aminobutyric acid; preparation of a test sample: dissolving the cosmetic test sample with hydrochloric acid, and filtering to obtain a test sample solution; preparation of a blank solution: filtering hydrochloric acid to obtain the blank solution; and measurement: detecting the reference solution, the test solution and the blank solution by using a separation column and a post-column derivatization reaction column so as to obtain the content of gamma-aminobutyric acid in the cosmetic test. The detection method is simple to operate, sensitive in response, high in accuracy and good in repeatability, and can be used for detecting the content of gamma-aminobutyric acid in cosmetics.

Description

technical field [0001] The invention relates to the technical field of γ-aminobutyric acid detection, in particular to a method for detecting the content of γ-aminobutyric acid in cosmetics. Background technique [0002] Gamma-aminobutyric acid (GABA), also known as aminobutyric acid, 4-aminobutyric acid, pipecolic acid, molecular formula C 4 H 9 NO 2 , molecular weight 103.1. Gamma-aminobutyric acid is an inhibitory neurotransmitter in the mammalian central nervous system. It is widely distributed in prokaryotic and eukaryotic organisms. Studies have shown that it has the effects of relieving anxiety, lowering blood pressure, improving sleep, removing wrinkles and whitening. Using this property of γ-aminobutyric acid at home and abroad, a series of products have been developed to give hope to beauty lovers. When the wrinkle removal concentration is in contact with the skin, uncomfortable symptoms such as tingling, numbness, itching, and burning will occur, and this side...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/067Y02P20/55
Inventor 刘英陈玉娟陈雯雯万金玉刘昌荣王浩马欣娟张坤郭学平
Owner BLOOMAGE BIOTECHNOLOGY CORP LTD
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