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Glucoside compound extracted and separated from lilium brownii and method and application of glucoside compound

A technology of glycosides and compounds, applied in the field of phytochemistry, can solve the problem of fewer reports of compounds, and achieve the effect of short time consumption

Pending Publication Date: 2021-09-03
CHENGDU PUSH BIOLOGICAL TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] It can be seen from the content of the above literature reports that the existing research on the chemical constituents of Liliaceae plants mainly focuses on polysaccharides and steroidal saponins, and relatively few reports on compounds of other structural types

Method used

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  • Glucoside compound extracted and separated from lilium brownii and method and application of glucoside compound
  • Glucoside compound extracted and separated from lilium brownii and method and application of glucoside compound
  • Glucoside compound extracted and separated from lilium brownii and method and application of glucoside compound

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Experimental program
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Effect test

Embodiment 1

[0048] A glycoside compound extracted and isolated from lily, the compound is a white powder, Molish reaction is positive, the molecular weight is 561, and the molecular formula is C 21 h 30 o 13 Cl 2 , has the structural formula shown in (I):

[0049]

[0050] The glycoside compound is from Lianda L. lancifolium The dried bulb extracts and separates and obtains, and concrete steps are as follows:

[0051] A. Reflux extraction

[0052] The dried lily is used as raw material, crushed and refluxed with 80-90% ethanol to obtain the extract;

[0053] B. Concentration under reduced pressure

[0054] The extract obtained in step A is decompressed to -0.08~-0.09MPa and concentrated to no alcohol, and then the concentrated extract is added with water for dispersion treatment to obtain an aqueous dispersion;

[0055] C. Resin column chromatography

[0056] The aqueous dispersion obtained in step B is wet-loaded, separated by AB-8 macroporous adsorption resin column chromat...

Embodiment 2

[0062] Take dried lilies (Lily L. lancifolium 10kg of dried bulbs), crushed to a particle size of 60 mesh, added 8 times the weight of ethanol with a concentration of 80wt% and refluxed for extraction 3 times, each time for 1 hour, and combined the extracts; decompressed the extracts to -0.08~-0.09MPa Concentrate until there is no alcohol, then disperse the concentrated extract by adding water at a volume ratio of 1:6 to obtain 20L of water dispersion; apply the water dispersion to the sample by wet method, and use AB-8 macroporous adsorption resin column chromatography to separate (methanol : water=50:50 V / V is the mobile phase), collect the chromatographic solution containing glycoside components, and then concentrate under reduced pressure to no alcohol to obtain 5L of concentrated solution; filter the concentrated solution, and use C18 reverse phase chromatographic packing for the filtrate under high pressure Preparation and separation (A: acetonitrile B: 0.1% V / V formic ...

Embodiment 3

[0066] Take dried lilies (Lily L. lancifolium 20kg of dry bulbs), crushed to a particle size of 70 meshes, added 8.5 times the weight of ethanol with a concentration of 85wt% and refluxed for extraction 4 times, each time for 1 hour, and combined the extracts; decompressed the extracts to -0.08~-0.09MPa Concentrate until there is no alcohol, and then disperse the concentrated extract by adding water at a volume ratio of 1:7 to obtain 36L of water dispersion; apply the water dispersion to the sample by wet method, and use AB-8 macroporous adsorption resin column chromatography to separate (methanol : water=50:50 V / V is the mobile phase), collect the chromatographic solution containing glycoside components, and then concentrate under reduced pressure to no alcohol to obtain 12L of concentrated solution; filter the concentrated solution, and use C18 reverse phase chromatographic packing for the filtrate under high pressure Preparation and separation (A: acetonitrile B: 0.1% V / V ...

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Abstract

The invention discloses a glucoside compound extracted and separated from lilium brownii and a method and application of the glucoside compound, and a structural formula of the glycoside compound is as shown in (I). The glycoside compound is extracted and separated by taking dry lilium brownii as a raw material by steps of ethanol reflux extraction, vacuum concentration, AB-8 resin column chromatography, C18 reversed-phase chromatographic separation and the like, is a novel glycoside compound with a novel structure and pharmacological activity, can be applied to preparation of an anti-lung cancer drug, and provides a reliable basis for the aspects of preparation of a new anti-cancer drug, medicinal research of glycoside substances and the like.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry, and in particular relates to a pharmacologically active glycoside compound extracted and separated from lily, as well as its preparation method and application. Background technique [0002] There are about 115 species of plants in the Liliaceae Liliaceae Lilium genus Lilium in the world, mainly distributed in northern temperate regions and subtropical mountains. There are 39 species and 26 varieties in China, of which 25 species and 19 varieties are special products in my country. Lily bulbs contain starch, which are not only edible, but also medicinal; flowers contain volatile oil, which can be used as spices. The 2020 edition of "Chinese Pharmacopoeia" records that the medicinal material of lily comes from Capsulata Lilium lancifolium Thunb., Lily L. brown nii F. E. Brown var. viridulum Baker and lilies L. Pumilum The dry bulb of DC. has the effect of nourishing yin and mois...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/18C07H1/08A61P35/00
CPCC07H15/18C07H1/08A61P35/00
Inventor 李波彭成闫婕谢晓芳郭大乐郑皓元刘丁吴涛王喜阔黄龙
Owner CHENGDU PUSH BIOLOGICAL TECH
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