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Application of a kind of plant lectin pha-1 in the preparation of anti-coronavirus medicine

A technology of phytohemagglutinin and coronavirus, applied in the field of preparation of anti-coronavirus drugs, phytohemagglutinin PHA-L, which can solve problems such as adverse reactions and urgent research and development of anti-highly pathogenic coronavirus drugs

Active Publication Date: 2022-03-11
NAT INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no specific drug specifically for the prevention and treatment of highly pathogenic coronaviruses (SARS-CoV-2, SARS-CoV, MERS-CoV), and clinically it is mainly based on supportive treatment and symptomatic treatment to improve symptoms. Therefore, the research and development of anti-highly pathogenic coronavirus drugs is urgent. A large number of drug screening experiments have been carried out for highly pathogenic coronaviruses worldwide, but the selected anti-coronavirus drug candidates only show narrow-spectrum activity, or Treatment is effective only at abnormally high doses, otherwise it will cause severe adverse reactions or immunosuppression (Zumla A, Chan J F W, Azhar E I, et al.Coronaviruses—drug discovery and therapeutic options[J].Nature reviews Drug discovery,2016,15( 5): 327-347.)
[0006] Since there is no targeted effective drug, the research and development of new drugs for highly pathogenic coronavirus is of great significance. In view of this, the purpose of the present invention is to provide a plant lectin PHA-L for preventing and treating highly pathogenic coronavirus infection There are no related reports on the application of phytohemagglutinin PHA-L to simultaneously inhibit SARS-CoV-2, SARS-CoV, and MERS-CoV

Method used

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  • Application of a kind of plant lectin pha-1 in the preparation of anti-coronavirus medicine
  • Application of a kind of plant lectin pha-1 in the preparation of anti-coronavirus medicine
  • Application of a kind of plant lectin pha-1 in the preparation of anti-coronavirus medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1 Phytohemagglutinin detection of SARS-CoV-2 S protein binding activity and antiviral activity

[0068] 1. Experimental materials

[0069] Lentil lectin, wheat germ agglutinin (WGA), sessile elderberry agglutinin (SSL), peanut lectin (Peanut lectin), Huaihuai agglutinin (MAL) were purchased from Wako (Japan) Datura agglutinin (DSL), succinyl-concanavalin A (succ-Con A), galactus agglutinin (GNL), phytohemagglutinin (PHA-E), leukolectin (PHA-L ), phytohemagglutinin-M (PHA-M) and phytohemagglutinin-P (PHA-P) from red kidney bean were purchased from Sigma Aldrich; SARS-COV-2 S trimer expressed in HEK293 cells was purchased from at ACRO Biosystems (China, Beijing); 293T cells were provided by the American Type Culture Collection (ATCC); Huh7 cells were provided by the Japanese Research Biological Resource Collection (JCRB); the SARS-CoV-2spike (Wuhan-Hu- 1, MN908947; hCoV-19 / South Africa / KRISP-K007869 / 2020, B.1.351, EPI_ISL_860630; hCoV-19 / England / QEUH-F56F0F / 2021...

Embodiment 2

[0082] Example 2 Detection of the Inhibitory Effect of Plant Hemagglutinin on the Infection of SARS-CoV-2 S Protein Mutants and SARS-CoV-2 Epidemic Mutants

[0083] In this example, the inhibitory effects of various plant lectins on the infection of SARS-CoV-2 S protein mutant strains and SARS-CoV-2 epidemic mutant strains, including the British epidemic mutant strain B.1.1.7, were detected. , South African popular mutant strain B.1.351, Brazilian popular mutant strain P.1, combined mutant strain D614G+I472V, combined mutant strain D614G+A435S, A222V, K417N, S477N, E484K, N501Y, combined mutant strain A222V+D614G, combined mutant strain Mutant strains such as strain K417N+D614G, combined mutant strain S477N+D614G, combined mutant strain E484K+D614G, combined mutant strain N501Y+D614G;

[0084] Lectins are mainly aimed at the glycosyl part of glycoproteins. In this example, the inventors studied whether the change of the glycation site of SARS-CoV-2 S protein would affect its n...

Embodiment 3

[0094] Example 3 Detection of Hemagglutination Activity and Cytotoxicity of Plant Hemagglutinin

[0095] Since many natural lectins can cause hemagglutination, in this example, the inventors studied the hemagglutination activity of different lectins, and studied the cytotoxicity of Huh7 and 293T cells.

[0096] 1. Detection of hemagglutination activity of phytohemagglutinin

[0097] Chicken blood samples were used to detect the hemagglutination activity of phytohemagglutinin, the rooster red blood cells were washed with PBS, and resuspended at a final concentration of 1% (v / v), and 50 μL of 2-fold serially diluted phytohemagglutinin solution was mixed with an equal volume of red blood cells The solution was mixed in a 96-well round-bottom plate, and a 2-fold serial dilution of influenza antigen (B / Maryland / 15 / 2016, NIBSC-UK-EN63QG, NIBSC code: 18 / 104, HA: 69 μg / mL) was performed and served as a positive control , PBS solution was used as a negative control, the plate was incu...

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Abstract

The invention discloses the use of a phytohemagglutinin PHA-L in the preparation of anti-coronavirus drugs. The present invention has been verified by experiments and found that the phytohemagglutinin PHA-L has better anti-highly pathogenic coronavirus activity, and can simultaneously Inhibition of SARS-CoV-2, SARS-CoV, MERS-CoV virus activity, shows that the phytohemagglutinin PHA-L can be used in the preparation of anti-coronavirus drugs, and has important prevention and / or treatment of high pathogenicity Application prospects of coronavirus infection.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the use of phytohemagglutinin PHA-L in the preparation of anti-coronavirus drugs. Background technique [0002] Infection with highly pathogenic coronaviruses is one of the public health problems that have attracted wide attention in recent years. These highly pathogenic coronaviruses include severe acute respiratory syndrome coronavirus 2 (Severs acute respiratory syndrome coronavirus 2, SARS-CoV-2), Severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus (MiddleEast respiratory syndrome coronavirus, MERS-CoV), with high transmissibility and high pathogenicity, through close contact Human-to-human transmission causes respiratory syndrome, with high morbidity and mortality, and has become a serious respiratory epidemic worldwide. [0003] Coronaviruses are divided into four genera: α, β, γ and δ. The highly pathogenic coronavi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/16A61P31/14
CPCA61K38/168A61P31/14
Inventor 王佑春王兰黄维金王文波李倩倩吴佳静武刚于传飞郭璐韵
Owner NAT INST FOR FOOD & DRUG CONTROL
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