Marker polypeptide for identifying pilose antler and antler of sika deer or red deer and application thereof

Abstract

The invention belongs to the technical field of biology, and particularly relates to a marker polypeptide for identifying pilose antler and antler of sika deer or red deer and application thereof. The marker polypeptide provided by the invention specifically comprises: a peptide fragment 1 which is as shown in SEQ ID No. 1: KAPQVSTPTLVELSR; and a peptide fragment 2 which is as shown in SEQ ID No. 2: HHGGEFTVVLQADFQK. The marker polypeptide and the detection method provided by the invention provide reference for searching marker peptide fragments in the related species lacking in the database. The marker polypeptide provided by the invention has excellent specificity and stability for pilose antler and antler of sika deer or red deer, is high in specificity, can be used for identifying pilose antler and antler medicinal materials, decoction pieces thereof and Chinese patent medicines fed by using raw powder, and has a good application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a characteristic polypeptide for identifying sika deer or red deer antler antlers and an application thereof. Background technique [0002] Antlers and antlers are traditional Chinese medicinal materials in my country, among which velvet is a sika deer (Cervus deer) Cervus nippon Temminck ) or red deer ( Cervus elaphus Linnaeus ) unossified young antlers of male deer with dense hair, which have the effects of strengthening kidney yang, benefiting essence and blood, strengthening muscles and bones, regulating Chong and Ren, and treating sores; Cervus nippon Temminck ) or red deer ( Cervus elaphus Linnaeus ) The horns that have been ossified or the horn bases that fall off in the next spring after sawing the velvet have the effects of warming kidney yang, strengthening bones and muscles, promoting blood circulation and reducing swelling. In traditional Chinese med...

AI Technical Summary

Problems solved by technology

However, in the existing technology, due to the lack of protein data of deer species, it is difficult to find target characteristic peptides, mainly due to the following points: 1. Sika deer, red deer and their counterfeit moose, reindeer, fallow deer, white-lipped deer, and white-tailed deer Equivalent to deer mammals, the genetic material has a high degree of similarity, and the corresponding proteins have a high degree of similarity, so it is difficult to find differences; 2. The natural population of sika deer is now only distributed in Northeast Asia, that is, the Ussuri region of Russia to China, China Taiwan, northern Vietnam, Japan and other places; while velvet and antler medicinal materials are only used on a large scale in China, there are few research materials on the proteomics of sika deer, and there is a lack of reference protein databases, especially the comparison of research data on collagen and keratin Few, it is difficult to find the differential peptides of different cervidae gums by comparing sequences in the library

At present, there is no method for identifying and accurately distinguishing sika deer, horses and deer from other cervids

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