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Bacillus subtilis capable of efficiently solubilizing phosphate and accelerating sprouting of Saccharum officinarum, and application of bacillus subtilis

A technology of Bacillus subtilis and bacterial fertilizer, which is applied in the field of microorganisms, can solve the problems of poor phosphorus dissolving effect, achieve the effect of increasing the content of nitrogen, phosphorus and potassium, good growth promotion, and meeting the needs of plant growth

Active Publication Date: 2021-08-13
YUNNAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sugarcane is an important sugar crop and energy crop, and there are a large number of endophytes in it, and there have been related reports to isolate endophytes from sugarcane, such as Bacillus subtilis, and use them for aspects such as dissolving phosphorus and promoting rooting. application, but the phosphorus dissolving effect is not good, and there is no relevant report that the related plant Bacillus subtilis can store in bud growth

Method used

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  • Bacillus subtilis capable of efficiently solubilizing phosphate and accelerating sprouting of Saccharum officinarum, and application of bacillus subtilis
  • Bacillus subtilis capable of efficiently solubilizing phosphate and accelerating sprouting of Saccharum officinarum, and application of bacillus subtilis
  • Bacillus subtilis capable of efficiently solubilizing phosphate and accelerating sprouting of Saccharum officinarum, and application of bacillus subtilis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Experimental materials

[0032] The root tissue of "sugarcane cultivar Yunzae 99-91" in the sugarcane resource nursery of Yunnan Agricultural University.

[0033] 2. Separation and identification

[0034] Using LB medium to isolate and purify from the base tissue of sugarcane, a strain was obtained, which was named ScBS1.

[0035] 3. Observation of culture characteristics

[0036] After culturing in LB solid medium, it was found that after the strain ScBS1 was cultured at 37°C for 10 hours, it could be clearly observed on the plate, with a bulge in the center, and similar watery secretions were observed inside the bulge, and the bulge disappeared after 24 hours. The shape is irregular and circular, the surface is not smooth, the edges are not smooth, yellowish white, and the diameter of the bacterial colony is 0.3-1cm (as shown in Figure 7).

[0037] 4. Physiological and biochemical characteristics identification

[0038] According to the identification method in...

Embodiment 2

[0043] Embodiment 2 Bacillus subtilis ScBS1 dissolving phosphorus and potassium dissolving ability

[0044] 1. Determination of phosphorus dissolving ability

[0045] The strain was inoculated with Ca 3 (PO 4 ) 2 In the liquid medium with poorly soluble phosphate as the substrate (insoluble phosphorus medium (NBRIP): glucose 10g / L, MgCl 2 ·6H 2 O 5.0g / L, MgSO 4 ·H 2 O 0.25g / L, KCl 0.2g / L, (NH 4 ) 2 SO 4 0.1g / L, insoluble phosphate or phosphate rock, add distilled water to 1000mL, adjust pH7.0~7.5, add distilled water to 1000mL, liquid does not add agar). By comparing the non-inoculated blank control, after inoculation of Bacillus subtilis ScBS1 bacteria, the soluble phosphorus content increased significantly, the first day was 39.40±1.88mg / L, the third day was 60.71±5.50, and the sixth day was 73.79±4.69mg / L, the soluble phosphorus content was the highest on the tenth day, reaching (164.09±4.94) mg / L. In the culture medium with calcium phytate as the substrate, the...

Embodiment 3

[0062] The growth-promoting effect of embodiment 3 Bacillus subtilis ScBS1 to sugarcane and other crops

[0063] 1. Experimental materials

[0064] Sugarcane seedlings and stems

[0065] 2. Preparation of bacterial solution

[0066] Bacterial culture: Put 10 μL of Bacillus subtilis ScBS1 original bacterial solution into the sterilized liquid LB medium with a pipette gun in the ultra-bacteria table irradiated with ultraviolet light for 30 minutes, seal the bottle mouth, and put it in a 37°C, 120rpm / min The multiplication of the bacteria in the shaker lasted for 72 hours.

[0067] Preparation of bacterial inoculum: After cultured at 37°C for 72 hours, the bacteria were centrifuged at 12000 rpm / 8min in a high-speed centrifuge to obtain culture-removed bacteria, resuspended with sterile water in a sterile clean bench, and diluted with a diluent Count by cloth plate method, determine the number of cells per unit volume of bacterial liquid, and configure a concentration of 10 5 ...

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Abstract

The invention discloses bacillus subtilis and application thereof, and belongs to the microbial technology field. The bacillus subtilis is collected in the Guangdong Microbial Culture Collection Center on July 15th, 2019 with the collection number of GDMCC NO: 60727, and the collection address is Guangdong Institute Of Microbiology, 5th floor, No. 59 building, No. 100, Xianlie Middle Road, Yuexiu District, Guangzhou. The bacillus subtilis disclosed by the invention is endophyte separated from Saccharum officinarum, the bacterial strain or the metabolite thereof has a good phosphate solubilizing and potassium degrading function and is favorable for improving the nutrient substance structure of soil, and meanwhile, the germination rate of the Saccharum officinarum or other crops can be obviously improved so as to be favorable for improving a growth promotion function.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of Bacillus subtilis capable of dissolving phosphorus efficiently and promoting germination of sugarcane and its application. Background technique [0002] Plant endophytes refer to a class of microorganisms that can survive from healthy plant living tissues without causing obvious pathological changes in host plants. They are a rich microbial resource, mainly including bacteria, fungi and actinomycetes. Therefore, the isolation of endophytes from living plant tissues has been reported. However, different plants or different strains isolated from the same plant have different functions or can produce different types of metabolites, which can be effectively used according to different needs. [0003] As people's dependence on chemical fertilizers gradually increases, while promoting plant growth and increasing production, it can bring many side effects, such as: soil acidif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P21/00C09K17/14C05G3/00C12R1/125C09K101/00
CPCA01N63/22C09K17/14C05G3/00C09K2101/00Y02W30/40
Inventor 李富生狄义宁谢林艳何丽莲刘鲁峰
Owner YUNNAN AGRICULTURAL UNIVERSITY
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