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Microcavity type digital PCR disc chip connected with silicon-based microvalve

A disc chip, silicon-based technology, applied in the field of microcavity digital PCR disc chip, can solve the problems of research disclosure, excessive moisture volatilization, etc., and achieve the effect of highly sensitive absolute quantitative detection

Pending Publication Date: 2021-08-13
SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the research on the disk chip mainly focuses on the multi-item joint inspection of one sample, but there is still no relevant research on issues such as excessive moisture volatilization.

Method used

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  • Microcavity type digital PCR disc chip connected with silicon-based microvalve
  • Microcavity type digital PCR disc chip connected with silicon-based microvalve
  • Microcavity type digital PCR disc chip connected with silicon-based microvalve

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Embodiment 1

[0023] Depend on figure 1 with 2 As shown, this embodiment provides a microcavity digital PCR disc chip connected with a silicon-based microvalve, including a disc 1 and several reaction units 2, and a single reaction unit 2 is sequentially arranged from the inside to the outside of the disc. Including a sample area connected by a connecting pipeline, a silicon-based microvalve and a silicon-based microcavity array; wherein, the silicon-based microvalve is composed of a first silicon substrate 201 and several parallel hydrophilic and oleophobic capillaries 202; the The silicon-based microcavity array is composed of a second silicon substrate 205 and tens of thousands of microcavities 206 with hydrophobic surfaces and hydrophilic inner walls of tens of thousands of nanoliters. Driven by centrifugal force, the aqueous phase such as the PCR master mix (containing extracted and purified target nucleic acid) first enters the silicon-based microcavity array through the hydrophilic...

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Abstract

The invention relates to a microcavity type digital PCR(digital Polymerase Chain Reaction,dPCR) disc chip connected with a silicon-based microvalve, which comprises a disc (1) and a plurality of reaction units (2), and each reaction unit (2) sequentially comprises a sample area, a silicon-based microvalve and a silicon-based microcavity array from inside to outside of the disc. Through centrifugal force and capillary force driving, the whole process that samples are spontaneously and orderly distributed to the microcavity digital PCR reaction unit is achieved, and then high-flux and high-sensitivity absolute quantitative detection of low-abundance nucleic acid samples can be achieved through a temperature control module and an optical detection system.

Description

technical field [0001] The invention belongs to the field of microfluidic chips, in particular to a microcavity digital PCR disc chip connected with a silicon-based microvalve. Background technique [0002] Digital PCR (digital Polymerase Chain Reaction, dPCR) is the third-generation PCR technology developed on the basis of conventional PCR and fluorescent quantitative PCR. As an in vitro DNA amplification technology, it has strong specificity, high sensitivity, absolute quantification, The characteristics of simplicity and speed have become an important detection method in the field of life sciences. However, the existing commercial dPCR platforms are limited by expensive equipment or complicated operations, making it difficult to achieve widespread application. Moreover, the current dPCR chip based on polydimethylsiloxane (polydimethylsiloxane, PDMS) material has problems such as excessive moisture volatilization and protein adsorption due to the permeability and hydropho...

Claims

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Application Information

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IPC IPC(8): C12M1/38C12M1/36C12M1/34C12M1/00B01L3/00
CPCB01L3/50273B01L3/502738C12Q1/6851C12Q2563/159C12Q2565/629
Inventor 冯世伦贾春平黄亚茹赵建龙
Owner SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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