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Sustained or controlled release antibacterial rice bran protein composite membrane as well as preparation method and application thereof

A technology of rice bran protein and rice bran acylated protein, which is applied in the field of food packaging, can solve the problems of undeveloped applications and few antibacterial films, and achieve the effects of long-term effect and timeliness, stable performance and extended shelf life

Active Publication Date: 2021-08-13
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are few researches on the preparation of antibacterial film by modifying rice bran protein combined with nanogel at home and abroad, and the application in food packaging materials is still to be developed

Method used

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  • Sustained or controlled release antibacterial rice bran protein composite membrane as well as preparation method and application thereof
  • Sustained or controlled release antibacterial rice bran protein composite membrane as well as preparation method and application thereof
  • Sustained or controlled release antibacterial rice bran protein composite membrane as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] This example is intended to illustrate a method of preparing a slow-release antibacterial rice bran protein composite film.

[0043] A method for preparing a slow-release antibacterial rice bran protein composite film, wherein the film base material is a carboxymethyl chitcan (purchased from Sigma) and rice branocycid protein, and antibacterial agent is curcumin (purchased in Mac, Mac) , Additive glycerol, Tween 20 is purchased in Mac, rice bran protein nanogel for laboratory, including:

[0044] (a) Rice bbf bf cruciacification: first addition of rice bran protein solution (2%) into phosphate buffer, constant temperature stirring simultaneously, adding succinic anhydride (15%), and then continues constant temperature (40 ° C) and stir 1.0 M NaOH maintains pH at 8.0, the reaction time is 30 min, and the reaction time is dialysis (the dialysis bag is 3.5 kD, 4 ° C, 24h), and freezing 12h in the refrigerator at -80 ° C, then transfer to -40 ° C The freeze drying machine was l...

Embodiment 2

[0053] This example is intended to illustrate a performance characterization of a slow-release antibacterial rice bran protein composite film.

[0054] The slow-release antibacterial rice bran protein composite membrane prepared in Example 1 was stored at a temperature of 25 ° C and a humidity of 55%, and various properties was measured (as a controlled protein film as a control).

[0055] 1. Determination of film thickness

[0056] The thickness of the film was measured by the screw micrometer, and the thickness of the film was measured. The average value is calculated, and the film thickness is calculated, and in accordance with a certain law, 5 points are random on the film to be filmed, and the result is accurate to 0.001 mm.

[0057] 2. Determination of tensile strength (TS) and fracture elongation (EB)

[0058] The mechanical properties of the anti-controlled release antibacterial rice bran protein composite film were analyzed using a tension tester (Labthink C610M, Shandong...

Embodiment 3

[0071] This example is used to illustrate the antibacterial activity of E. coli by reseudently release the antibacterial rice bran protein composite membrane.

[0072] The composite film having a better composite performance in Example 2, that is, W (ARBP): W (CMCs) is 3: 1, and the amount of ARBPNG is 3%, 5% and 10%, and the antibacterial experimental test of the present embodiment is carried out. Among them, a control is made in a composite film without ARBPNG).

[0073] The antibacterial activity of the composite membrane is used to test the antibacterial activity of E. coli. Press the composite film film 2 × 2 cm 2 Slices of sheets, placed in solution (0.1%, w / v) containing 20 ml of protein (0.1%, w / v), E. coli concentration is 10 3 CFU / ml of centrifuge tubes. The centrifuge tube was incubated in a TS-2102C oscillator; oscillating at 37 ° C and 150 rpm for 24 hours. 0.5 ml of culture solution was collected every 2 hours, and in a 96-well plate was added, the absorbance v...

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Abstract

The invention discloses a sustained and controlled release antibacterial rice bran protein, which composite membrane and a preparation method and an application thereof. The composite membrane is prepared through the following steps that rice bran protein is subjected to succinylation modification through succinic anhydride to obtain rice bran acylated protein, the rice bran acylated protein and carboxymethyl chitosan serve as main membrane base material raw materials, glycerin serves as a plasticizer, tween 20 is a dispersant, and curcumin-loaded rice bran nanogel is added at the same time, according to the present invention, the film base material raw materials are combined according to the specific ratio, and the raw material components achieve the synergistic effect, such that the composite film has the excellent antibacterial property and the excellent mechanical property, and the sustained and controlled release effect of the antibacterial agent in the composite film is achieved; and the composite film produced by the invention is uniform in texture and excellent in performance. As the film base material is a food-borne raw material, the film has the characteristics of edibility, environmental protection, low energy consumption and the like, is a novel food packaging film which is effective and has a wide application prospect, and has a far-reaching influence on the development of the field of food packaging.

Description

Technical field [0001] The present invention relates to the field of food packaging technology, and more particularly to a slow-release antibacterial rice bran protein composite film and a preparation method thereof. Background technique [0002] At present, most of the food packaging is a non-degradable plastic product, which has a huge challenge for the living environment. With the implementation of international banned, degradable packaging materials and natural non-toxic edible packaging materials have gradually become a research hotspot in the field of food packaging. Many researchers have begun to use natural polymer materials to form a formable film for film forming substrates to replace traditional plastic packaging: On the one hand, the natural polymer material is prepared for the production of safety, environmentally-friendly and degradable biofilms. I have gradually emerged; on the other hand, the launch of antibacterial packaging makes food quality more reliable, and ...

Claims

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Application Information

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IPC IPC(8): C08J5/18C08L89/00C08L5/08C08K5/053C08K5/132C08H1/00
CPCC08J5/18C08H1/00C08J2389/00C08J2305/08C08J2405/08C08K5/053C08K5/132
Inventor 郭鸣鸣刘东红邹智鹏叶兴乾张欣慧何巧
Owner ZHEJIANG UNIV
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