Application of D-pinitol in promoting growth of panax notoginseng and inducing resistance
A technology for inducing resistance and Panax notoginseng, which is applied in the fields of application, plant growth regulators, plant growth regulators, etc., can solve problems such as the reduction of fertilizer efficacy and the destruction of agricultural ecological environment, so as to promote germination and growth, and improve stress resistance Sex, increase the effect of fresh weight per plant
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Embodiment 1
[0047] Embodiment 1: D-pinitol promotes germination and growth test of Panax notoginseng seeds
[0048] This test adopts the method of sand-cultured Panax notoginseng in bottles, and adopts analytical-grade purity D-pinitol as the experimental material. First, 1 g of D-pinitol is added to 1 L of sterile water to prepare a 1000 mg / L mother liquor, and then gradually diluted to The solutions of 1, 5, 20, and 50 mg / L were used for later use, and sterile water was used as a control treatment; 100 g of sterilized quartz sand was placed in a glass tissue culture bottle and then dried in an oven at 141 ° C for 4 h to sterilize. After cooling, add 18ml of D-pinitol solution with a concentration of 0, 1, 5, 20, and 50mg / L respectively, stir evenly, and plant 12 Panax notoginseng seeds. On the 60th day, start to investigate the emergence of the seedlings, and investigate the fresh weight index of the Panax notoginseng single plant on the 90th day. The test results are shown in figure ...
Embodiment 2
[0050] Embodiment 2: D-pinitol treatment continuous cropping soil bottle planting test of Panax notoginseng
[0051] The method of using potted continuous cropping soil for long-term watering: the notoginseng continuous cropping soil is taken from the notoginseng farmland in Wenshan, Yunnan, and the retrieved continuous cropping soil is dried in the shade, then passed through a 40-mesh sieve to remove plant residues in the soil, and weigh 2.5 kg The continuous cropping soil was added to the finishing box (28×20×17cm) for later use; 1g of analytically pure D-pinitol was added to 1L of sterile water to prepare a 1000mg / L mother solution, and then gradually diluted to 1, 10, 50, 100mg / L solution, with sterile water as the control treatment; take 500ml of the above solution and add it into different finishing boxes, then cover the lid of the finishing box, and seal it with parafilm, and place the finishing box in a cool place away from light. Set up 3 replicates (eg figure 2 sh...
Embodiment 3
[0053] Embodiment 3: D-pinitol induces resistance test to Panax notoginseng
[0054] The method of spraying treatment with D-pinitol: place the potted plants of Panax notoginseng after transplanting and surviving in a transparent plastic airtight incubator, transplant 10 plants of Panax notoginseng in each pot, and use sterile water and D-pinitol at a concentration of 1mg / L respectively. - Pinitol sprayed the potted Panax notoginseng, treated with sterile water as the control group, and D-pinitol as the treatment group; the leaves were sprayed with the above liquid every day, after 5 days of continuous treatment, the leaves were challenged to inoculate Panax notoginseng black Bacteria cake of Alternaria panax, after inoculation, moved to a transparent plastic incubator for moisture cultivation, collected diseased leaves 3 days after inoculation, used Epson Perfection V850 Pro scanner to scan diseased leaves, and used Adobe Photoshop software to intercept and calculate The rela...
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