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A kind of biological preparation method of blue pigment

A technology for biological preparation and blue pigment, applied in the field of biological preparation of blue pigment, can solve the problems of inability to realize industrialization and industrialization, and achieve the effects of economical convenience, less pollution, safe and stable quality

Active Publication Date: 2022-03-11
南京合谷生命生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Blue pigment, the current main production method is to insert the Indigoidine synthetic gene into a suitable Escherichia coli expression vector (such as pET28) and transfer the plasmid containing the target gene fragment into the expression host Escherichia coli by transformation, generally using shake flasks way to produce blue pigment, but can not realize industrialization and industrialization

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  • A kind of biological preparation method of blue pigment

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Experimental program
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Effect test

Embodiment 1

[0023] A kind of biological preparation method of blue pigment, concrete steps are as follows:

[0024] (1) Strain activation: add the recombinant E. coli strain into the medium shaking tube, and place it on a horizontal shaker for overnight cultivation at a temperature of 35°C and a rotation speed of 170 rpm to obtain activated E. coli;

[0025] (2) Expansion culture: Inoculate activated E. coli with 1% (v / v) inoculum into 1L of fermentation medium in the Erlenmeyer flask for expansion culture at a temperature of 35°C. The fermentation medium includes 5.8 g / L tryptone, 3.6 g / L yeast extract, 1.4 g / L K 2 HPO 4 , 2.2 g / L KH 2 PO 4 , 1 ml / L glycerol, until the bacterial density OD600 value is 12, to obtain the inoculation seed liquid;

[0026] (3) Fermentation: Place the inoculated seed solution in a fermentation tank for continuous fermentation at a temperature of 35°C and a rotation speed of 200rpm. During the fermentation process, sterile air is introduced, and the ferme...

Embodiment 2

[0031] A kind of biological preparation method of blue pigment, concrete steps are as follows:

[0032] (1) Strain activation: add the recombinant Escherichia coli strain into the medium shaking tube, and place it on a horizontal shaker for overnight cultivation at a temperature of 38°C and a rotation speed of 200rpm to obtain activated Escherichia coli;

[0033] (2) Expansion culture: Inoculate activated Escherichia coli with 10% (v / v) inoculum into 1L of fermentation medium in the Erlenmeyer flask for expansion culture at a temperature of 38°C. The fermentation medium includes 5.8 g / L tryptone, 3.6 g / L yeast extract, 1.4 g / L K 2 HPO 4 , 2.2 g / L KH 2 PO 4 , 1 ml / L glycerol, until the bacterial density OD600 value is 18, to obtain the inoculation seed liquid;

[0034] (3) Fermentation: Place the inoculated seed liquid in a fermenter for continuous fermentation at a temperature of 38°C and a rotation speed of 1200rpm. During the fermentation process, sterile air is introdu...

Embodiment 3

[0039]A kind of biological preparation method of blue pigment, concrete steps are as follows:

[0040] (1) Strain activation: add the recombinant Escherichia coli strain into the medium shaking tube, and place it on a horizontal shaker for overnight cultivation at a temperature of 36°C and a rotation speed of 180rpm to obtain activated E. coli;

[0041] (2) Expanded culture: Inoculate activated E. coli with 8% (v / v) inoculum into 1L of fermentation medium in the Erlenmeyer flask for expanded culture at a temperature of 36°C. The fermentation medium includes 5.8 g / L tryptone, 3.6 g / L yeast extract, 1.4 g / L K 2 HPO 4 , 2.2 g / L KH 2 PO 4 , 1 ml / L glycerol, until the bacterial density OD600 value is 16, to obtain the inoculation seed liquid;

[0042] (3) Fermentation: Place the inoculated seed liquid in a fermenter for continuous fermentation at a temperature of 36°C and a rotation speed of 800rpm. During the fermentation process, sterile air is introduced, and the fermentati...

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Abstract

The invention discloses a biological preparation method of blue pigment. Recombinant Escherichia coli is used as a strain, activated, and the activated Escherichia coli is inserted into a fermentation medium for expanded culture, and then placed in a fermentation tank for continuous fermentation. Enter sterile air, and maintain the pH value of the fermentation broth within the range of 6.8 to 7.2. When the content of residual sugar in the fermentation tank is less than 1 g / L, feed the material, continue to ferment for a period of time, and separate and purify to obtain blue pigment. The biological preparation method of the blue pigment of the invention realizes the mass production of the blue pigment, has less pollution, is economical and convenient, and is suitable for the fields of cosmetics, food, health care and the like.

Description

technical field [0001] The invention relates to a biological preparation method of cyanine, belonging to the technical field of cyanine preparation. Background technique [0002] Blue pigment is a kind of non-toxic microbial natural blue pigment. The dye has pure shade and high dyeing strength. Because of its natural non-toxicity, it can also be used in food pigments. Its molecular formula is C 10 h 8 o 4 N 4 , the relative molecular mass is 248, and the structural formula is as follows: . Blue pigment is mainly used in the dye industry. At present, the dyestuff used in the dye industry is still mainly industrial dye indigo, but the production of industrial indigo is mainly based on chemical synthesis, and the pollution produced is relatively serious, which is one of the important sources of industrial pollution. First, the production of blue pigment by microbial fermentation has less pollution and lower cost, and has gradually become an alternative to industrial indi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/16C12N1/21C12R1/19
CPCC12P17/165C12N1/20
Inventor 孙磊李亚
Owner 南京合谷生命生物科技有限公司
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