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Freeze-drying protective agent for prolonging normal-temperature preservation of probiotics and freeze-drying method

A technology of freeze-drying protective agent and normal temperature storage, which is applied to the preservation of microorganisms, etc., and can solve the problems of heavy workload, cumbersome methods, and high cost

Inactive Publication Date: 2021-08-10
安徽善和生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In practical application, there are the following deficiencies in prolonging the storage of probiotics at room temperature: in practical application, the effect of freeze-drying protective agent is very different due to the difference in bacterial species, material composition and proportion, and storage conditions. Therefore, it is urgent to develop a cryoprotectant that can prolong the storage of probiotics at room temperature.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A kind of freeze-drying method of the freeze-drying protectant that prolongs probiotic normal temperature preservation, comprises the steps:

[0037] Step 1: Take raw materials in parts by weight, including: 17 parts of trehalose, 5 parts of linolenic acid, 10 parts of mannitol, 6 parts of serum albumin, 0.01 part of surfactant, 0.01 part of defoamer, sodium chloride 1 part, 2 parts of sodium citrate and 10 parts of lentinan;

[0038] Step 2: Add 1 part of glucoamylase and probiotic bacteria liquid to the mixture in step 1 to make a freeze-dried reagent;

[0039] Step 3: Place the freeze-dried reagents under the condition of vacuum degree of 0.01 mbar, respectively, at the temperature of -20°C for 2 hours, at the temperature of 15°C for 10 hours, and at the temperature of 10°C for 1 hour.

Embodiment 2

[0041] A kind of freeze-drying method of the freeze-drying protectant that prolongs probiotic normal temperature preservation, comprises the steps:

[0042] Step 1: Take raw materials in parts by weight, including: 18 parts of trehalose, 6 parts of linolenic acid, 10 parts of mannitol, 7 parts of serum albumin, 0.01 part of surfactant, 0.01 part of defoamer, sodium chloride 1 part, 3 parts of sodium citrate and 11 parts of lentinan;

[0043] Step 2: Add 1 to 3 parts of glucoamylase and probiotic bacteria liquid to the mixture in step 1 to make a freeze-dried reagent;

[0044] Step 3: The lyophilized reagents were placed in a vacuum of 0.01 mbarr, at a temperature of -20°C for 2.5 hours, at a temperature of 18°C ​​for 15 hours, and at a temperature of 10°C for 1.2 hours.

Embodiment 3

[0046] A kind of freeze-drying method of the freeze-drying protectant that prolongs probiotic normal temperature preservation, comprises the steps:

[0047] Step 1: Take raw materials in parts by weight, including: 19 parts of trehalose, 10 parts of linolenic acid, 10.5 parts of mannitol, 7 parts of serum albumin, 0.01 part of surfactant, 0.01 part of defoamer, sodium chloride 1.2 parts, 3 parts of sodium citrate and 12 parts of lentinan;

[0048] Step 2: Add 1 to 3 parts of glucoamylase and probiotic bacteria liquid to the mixture in step 1 to make a freeze-dried reagent;

[0049] Step 3: Place the freeze-dried reagents at a vacuum of 0.01 mbar and at a temperature of -20°C for 2.5 hours, at a temperature of 20°C for 1.5 hours, and at a temperature of 10°C for 1.5 hours.

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Abstract

The invention discloses a freeze-drying protective agent for prolonging normal-temperature preservation of probiotics and a freeze-drying method, and belongs to the technical field of probiotic production. The freeze-drying protective agent is prepared from, by weight, 17-21 parts of trehalose, 1-3 parts of saccharifying enzyme, 5-12 parts of linolenic acid, 10-11 parts of mannitol, 6-9 parts of serum albumin, 0.01-0.02 part of surfactant, 0.01-0.03 part of defoaming agent, 1-3 parts of sodium chloride, 2-5 parts of sodium citrate and 10-15 parts of lentinan. By adding linolenic acid, sodium chloride and sodium citrate, the freeze-drying effect of trehalose and mannitol at normal temperature can be effectively prolonged, the freeze-drying effect of the freeze-drying protective agent is enhanced, the service life of the freeze-drying protective agent is prolonged, and the freeze-drying protective agent is simple in process, low in cost and convenient to popularize and use.

Description

technical field [0001] The invention relates to a freeze-drying protective agent and a freeze-drying method for prolonging the normal-temperature storage of probiotics, belonging to the technical field of probiotics production. Background technique [0002] Probiotics are a class of active microorganisms that are beneficial to the host. They are a general term for active beneficial microorganisms that colonize the human intestinal tract and reproductive system and can produce definite health effects, thereby improving the host’s micro-ecological balance and exerting a beneficial effect on the intestinal tract. Beneficial bacteria or fungi in animals mainly include: Clostridium butyricum, lactic acid bacteria, bifidobacteria, Lactobacillus acidophilus, actinomycetes, yeast, etc. The most powerful products researched in the world are mainly the above-mentioned types of microorganisms Composed of compound active probiotics, it is widely used in the fields of bioengineering, ind...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04
CPCC12N1/04
Inventor 周英刘帅茅露燕
Owner 安徽善和生物科技有限公司
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