PCR-based continuous liquid feeding device

Abstract

The invention relates to a continuous liquid feeding device based on PCR. The device includes a sample adding layer and a motor pump; The sample adding layer includes a reagent tube and a piston, one end of the reagent tube is provided with a sealing structure, and the other end is provided with a piston, and the piston rod of the piston is fixedly connected with the connecting shaft of the motor pump; the motor pump includes a motor unit and a connecting shaft, and the output of the motor unit The shaft and the connecting shaft are fixedly connected. Through the driving action of the motor pump, the connecting shaft connected to the motor unit is controlled to drive the piston in the reagent tube to reciprocate along the tube wall, so as to push the reagent in and suck out the reagent. The motor unit includes multiple sets of motors, and the corresponding reagent tube also includes several First, during use, the liquid in each reagent tube is different, and the timing of its push-in or suction is also different, so as to realize the continuity of reaction and the continuity of adding different reagents.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a continuous liquid feeding device based on PCR. Background technique [0002] In scientific experiments such as biology, chemistry, and materials, it is often necessary to operate on fluids, such as the preparation of sample DNA, liquid chromatography, PCR reaction, and electrophoresis detection, etc., are all performed in a liquid-phase environment. If the steps of sample preparation, biochemical reaction, and result detection are to be integrated on the biochip, the amount of fluid used in the experiment will be reduced from milliliters and microliters to nanoliters or picoliters. At this time, powerful microfluidic devices are necessary. Indispensable. Therefore, with the development of biochip technology, microfluidic technology, as a key supporting technology of biochip, has also received more and more attention. [0003] The purification and recovery of nucleic acid i...

AI Technical Summary

Problems solved by technology

The combination of pipeline structure and electrodes is used, not only the complex structure makes the production of the pipeline layer more difficult, but also the setting of the electrodes is cumbersome, there are many factors to consider, and it is not easy to realize

At the same time, the internal pipeline of the chip is not integrated, and the reaction solution is difficult to mix and test as expected, and usually the extraction, purification, and amplification of nucleic acid need to be completed by using different instruments, which is cumbersome to operate

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