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Bio-ink composition for 3D printing, containing human-derived component and having tissue-specific cell differentiation effect, and preparation method therefor

A bio-ink and 3D printing technology, applied in applications, inks, household appliances, etc., to achieve high cell affinity and improve mechanical properties

Pending Publication Date: 2021-07-30
爱恩斯生物科技(昆山)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to manufacture liquid hydrogels, the extracellular matrix currently used in bioinks is used in a state of being dissolved in pepsin and acetic acid or hydrochloric acid, so there is a limit to improving mechanical properties with the extracellular matrix itself

Method used

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  • Bio-ink composition for 3D printing, containing human-derived component and having tissue-specific cell differentiation effect, and preparation method therefor
  • Bio-ink composition for 3D printing, containing human-derived component and having tissue-specific cell differentiation effect, and preparation method therefor
  • Bio-ink composition for 3D printing, containing human-derived component and having tissue-specific cell differentiation effect, and preparation method therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0078] Hereinafter, the present invention will be described more specifically by way of examples. However, the scope of the present invention is not limited to the following examples, and those skilled in the art should understand that various variations, modifications, or applications can be made within the scope of the technical idea derived from the contents of the claims.

Embodiment 1

[0080] Example 1: Fabrication of bioinks comprising micronized acellular dermal matrix (Micronized Acellular Dermal Matrix, M-ADM) tissue

[0081] (1) Manufacture of M-ADM powder

[0082] Decellularized skin tissue (collected from cadavers donated by tissue banks for non-profit patient treatment).

[0083] First, the skin tissue is subjected to a degreasing process using alcohol and hexane. Thereafter, the epidermis and cells were removed using sodium hydroxide (NaOH), and the 2 S) Remove the hair sticking to the tissue. Then, after hydrogen peroxide (H 2 o 2 ) and then neutralized and washed with distilled water. The washed skin tissue was freeze-dried to remove water, and the tissue was pulverized using a micro pulverizer. In the pulverization, a dermal tissue powder of 500 μm or less is produced by passing through a sieve with a diameter of 250-500 μm.

[0084] The prepared tissue in powder form is sterilized.

[0085] (2) Manufacture of M-ADM-based bioink

[0086]...

Embodiment 2

[0090] Example 2: Fabrication of bioinks comprising micronized acellular cartilage (M-AC) tissue

[0091] (1) Manufacture of M-AC powder

[0092] Cartilage tissue (collected from cadavers donated from tissue banks for non-profit patient treatment) was pulverized using a micro-pulverizer. In the crushing, the cartilage tissue powder below 500 um is obtained through a sieve with a diameter of 250-500 um.

[0093] Thereafter, decellularization was performed after obtaining cartilage tissue powder.

[0094] First, a degreasing process is performed on the cartilage tissue using alcohol and hexane. Thereafter, cells were removed with sodium hydroxide (NaOH), and washed with ethanol (Ethanol) and distilled water. After removing water by freeze-drying, the tissue in powder form is sterilized with an electron beam.

[0095] (2) Fabrication of M-AC-based bioink

[0096] The M-AC powder prepared in (1) and the alginate aqueous solution were mixed to prepare a bioink composition in t...

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Abstract

The present invention relates to a bio-ink composition for three dimensional (3D) printing, and a preparation method therefor. The present invention can provide: a bio-ink composition having a synergistic effect on the differentiation of cells into a specific tissue according to tissue specificity of a human-derived component contained in the bio-ink composition; and a preparation method therefor.

Description

technical field [0001] The present invention relates to a bioink composition for three-dimensional (3D, Three dimensional) printing and a method for producing the same, and in more detail, relates to cell differentiation into a specific tissue according to the tissue specificity of human tissue-derived components contained in the bioink composition Synergistic bioink compositions and methods for their manufacture. Background technique [0002] Tissue engineering is a discipline that uses biology, engineering, and material engineering as the basis to manufacture human tissues and organs to replace lost or damaged tissues. Due to industrialization and aging, the number of patients with tissue loss or damage is increasing, and thus the field of tissue engineering for replenishing lost or damaged tissue is expanding. Among them, an area that has recently received attention is 3D bioprinting. [0003] 3D bioprinting is to scan the target structure in three dimensions and obtain...

Claims

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Application Information

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IPC IPC(8): C09D11/101
CPCC09D11/101
Inventor 李索元姝允金炯九李焕铁
Owner 爱恩斯生物科技(昆山)有限公司
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