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Fermentation method of bacillus velezensis strainSUNO-18S-36

A technology of SUNO-18S-36 and Bacillus velesii, which is applied in the field of microorganisms, can solve the problems of lack of microorganisms and fermentation methods with high live bacteria content, achieve high live bacteria content, high spore rate, and improve stability Effect

Active Publication Date: 2021-07-30
苏农(广德)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, there is a lack of microorganisms that can simultaneously decompose phosphorus, degrade pesticide residues, and have an antagonistic effect on plant pathogens, and there is even a lack of fermentation methods for such microorganisms with high efficiency and high live bacteria content.

Method used

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  • Fermentation method of bacillus velezensis strainSUNO-18S-36
  • Fermentation method of bacillus velezensis strainSUNO-18S-36
  • Fermentation method of bacillus velezensis strainSUNO-18S-36

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Screening and identification of strains

[0037] I. Separation, screening of strains

[0038] Material and method

[0039] 1.1 sample

[0040] Typical fields of tomato sterilization strains, three root soil samples;

[0041] Generally, there are 2 strains of watermelon, and 3 root soil samples;

[0042] 3 strains of tomato strains of salted fields, 5 root soil samples;

[0043] 18 total samples.

[0044] 1.2 medium

[0045] LB liquid medium: protein 胨 10g, yeast extract 5g, sodium chloride 10g, reolified to 1 L after dissolving with water, adjusting pH to 7.0 to obtain LB liquid medium. 15 g of agar powder was added to the LB liquid medium to obtain LB solid medium.

[0046] PDA liquid medium: In the 200 g potato of peeled small pieces, 800 ml of water is added to boiling, maintaining 20 to 30 min, filtrate with gauze, and filtrates with water supply volume to 1000 ml, PDA liquid medium . 15 g of agar powder was added to the PDA liquid medium to give a PDA solid m...

Embodiment 2

[0088] Example 2 Nature of Bacillus Velezensis Suno-18S-36 strains

[0089] I. tolerance to acid alkaline and salinity

[0090] Material and method

[0091] 1.1 SUNO-18S-36 strain activation: From the beveled from the Belles Bacillus SUNO-18S-36 strains, some bacteria were collected in a shake flask to contain LB liquid medium with a sterile vaccination needle (250ml shake bottle The liquid volume was 50 mL), and after 24 h of 30 ° C, 150 r / min was cultured, the Lb culture was turbid, and for use.

[0092] 1.2 The pH is adjusted with aqueous hydrochloric acid or NaOH aqueous solution of hydrochloric acid or NaOH, respectively, in a 250 ml triangular bottle, respectively. The medium was 50 mL, and each pH test was repeated. After the medium of each pH was sterilized, cooled to room temperature, and if the pH was changed, the pH was adjusted to approximate the quartz value, and the measured pH was recorded. After sterilization and regulating the pH, 1 ml / bottle of the cultured b...

Embodiment 3

[0146] Example 3 Effect of Different Cultural Bases on Fermented Fungus Content and Spid

[0147] Ferrated fermentation medium A, B and C.

[0148]The fermentation medium A contains: protein 12g / L, yeast powder 4g / L, glucose 50 g / L, sodium chloride 12g / L, magnesium chloride 0.7 g / L, potassium phosphate 0.7 g / L and calcium carbonate 3g / L, The pH 7.2.50 lifting the fermentor is 70%.

[0149] The fermentation medium B contains 15 g / l, corn starch 20 g / l, glucose 10 g / L, calcium carbonate 2 g / l, ammonium sulfate 0.2 g / L, and seven water sulfate 0.2 g / L, pH 7.2. 50 liters of fermentors are 70%.

[0150] Fermentation medium C contains: glucose 5g / L, corn powder 12g / L, yeast powder 12g / L, silkworm powder 7.5 g / L, calcium carbonate 7.5 g / L, 水 5g / L, calcium phosphate 5g / L, pH 7.2.50 liter of fermentor is 70%.

[0151] The seed fluid of the Bakelus Velezensis SUNO-18S-36 strain was connected to a 50 liter of fermentrium having each medium, and the cul...

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Abstract

The invention provides a fermentation method of a bacillus velezensis strain SUNO-18S-36, and relates to the field of microorganisms. According to the fermentation method, a culture medium is adopted and comprises 4-10 g / L of glucose, 5-20 g / L of corn steep liquor, 5-25 g / L of yeast powder, 5-10 g / L of silkworm chrysalis meal, 0-10 g / L of calcium carbonate, 0-30 g / L of calcium gluconate, 0.1-1 g / L of magnesium sulfate heptahydrate, 0-0.1 g / L of manganese sulfate and 0-10 g / L of calcium phosphate, and the pH is 7.0-7.5. The microorganisms involved in the fermentation method have the functions of dissolving phosphorus and degrading pesticide residues and have an antagonistic effect on pathogenic bacteria, and by adopting the fermentation method, fermentation liquor with high viable bacterium content, high spore rate and high bacteriostatic active substances can be obtained within a relatively short time.

Description

Technical field [0001] The present invention relates to the field of microorganisms, and more particularly to a fermentation method involving a SUNO-18S-36 strain of Belce Bacillus. Background technique [0002] In the prior art, it is reported that the application of Belles buds in straw degradation and sewage ammonia nitrogen degradation, and some people have reported that there is also a report in aluminum toxic soil repair, alleviating aluminum toxic stress and increase plant antibiotic toxic stress. Bacillus that prevents roots of peanuts. However, in the prior art, the lack of phosphorus can be given simultaneously, degradable pesticide residues, microorganisms with antagonistic effects on plant pathogens, and lack of high-efficiency and high-acting fermentation methods of microorganisms. Inventive content [0003] It is an object of the present invention to provide a fermentation method of Bacillus Velezensis Suno-18S-36 strain, which has a function of prymentary, degrade...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/07
CPCC12N1/20
Inventor 王晓军邹玲玲丰超王玲丽李勇
Owner 苏农(广德)生物科技有限公司
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